comparison README.md @ 30:6669fd407dc9 draft default tip

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This is the repository associated with the VAPPER Galaxy Tool available in the Galaxy Toolshed
VAPP accurately quantifies the variant antigen diversity in Trypanosoma congolense isolates or the variable Cog presence in T.vivax isolates


The Trypanosoma congolense variant antigen repertoire is divided into 15 clades or phylotypes. These phylotypes are present in any T. congolense isolate, but their relative abundance varies between strains. The purpose of the VAPPER is to accurately quantify antigen diversity in any T. congolense isolate by calculating the relative frequency of each phylotype. 

The Galaxy VAPPER Tool has four modes. 
1) T.congolense Genomic:

This requires NGS paired reads and uses bowtie2 and samtools for read mapping and processing, cufflinks for transcript abundance estimation, and hmmer for sequence identification. The output is a stacked bar chart and a table of frequencies based on the transcript abundances.

3) T.vivax clusters of orthologs
The approach for T. vivax relies on the presence/absence of clusters of orthologs (COGs). It requires velvet for the genome assembly and blast. it recieves paired sequencing reads in fastq format (or a contig file if already assembled) and the output is a binary matrix of the presence/absence of each COG/gene for a given sample. Within the tool there is a database of 28 isolates that are used as a comparison producing a heatmap and dendogram.

4) T.vivax Transcriptomic pathway
This method takes two NGS paired reads, maps the transcripts against a user supplied reference file and then estimates the trancript aboundance (using samtools and cufflinks). The output is then binned into 175 predefined phylotypes based on their greatest blastx similarity (this table is available in the results directory). A pident of less than 70%  is considered unidentified. The results provide a tabular and graphical phylotype profile of the sample(s)

30:6669fd407dc9

This is the repository associated with the VAPPER Galaxy Tool available in the Galaxy Toolshed
VAPP accurately quantifies the variant antigen diversity in Trypanosoma congolense isolates or the variable Cog presence in T.vivax isolates

The Trypanosoma congolense variant antigen repertoire is divided into 15 clades or phylotypes. These phylotypes are present in any T. congolense isolate, but their relative abundance varies between strains. The purpose of the VAPPER is to accurately quantify antigen diversity in any T. congolense isolate by calculating the relative frequency of each phylotype. 

The Galaxy VAPPER Tool has four modes. 
1) T.congolense Genomic:

This requires NGS paired reads and uses bowtie2 and samtools for read mapping and processing, cufflinks for transcript abundance estimation, and hmmer for sequence identification. The output is a stacked bar chart and a table of frequencies based on the transcript abundances.

3) T.vivax clusters of orthologs
The approach for T. vivax relies on the presence/absence of clusters of orthologs (COGs). It requires velvet for the genome assembly and blast. it recieves paired sequencing reads in fastq format (or a contig file if already assembled) and the output is a binary matrix of the presence/absence of each COG/gene for a given sample. Within the tool there is a database of 28 isolates that are used as a comparison producing a heatmap and dendogram.

4) T.vivax Transcriptomic VAP 
This method takes two NGS paired reads, maps the transcripts against a user-supplied reference file and
then estimates the trancript aboundance (using samtools and cufflinks). The output is then binned into 
175 predefined phylotypes based on their greatest blastx similarity (this table is available in the results). A pident of less than 70% is considered unidentified.
The results are shown as tabular and graphical phylotype profiles. The user-supplied reference file should contain assembled transcripts. We advise it to be created using Trinity (Grabherr et al. 2013).