Mercurial > repos > ktnyt > gembassy
comparison GEMBASSY-1.0.3/doc/html/gp2.html @ 0:8300eb051bea draft
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author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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1 <!--START OF HEADER - DON'T ALTER --> | |
2 | |
3 <HTML> | |
4 <HEAD> | |
5 <TITLE> EMBOSS: gp2 </TITLE> | |
6 </HEAD> | |
7 <BODY BGCOLOR="#FFFFFF" text="#000000"> | |
8 | |
9 | |
10 | |
11 <table align=center border=0 cellspacing=0 cellpadding=0> | |
12 <tr><td valign=top> | |
13 <A HREF="/" ONMOUSEOVER="self.status='Go to the EMBOSS home page';return true"><img border=0 src="http://soap.g-language.org/gembassy/emboss_explorer/manual/emboss_icon.jpg" alt="" width=150 height=48></a> | |
14 </td> | |
15 <td align=left valign=middle> | |
16 <b><font size="+6"> | |
17 gp2 | |
18 </font></b> | |
19 </td></tr> | |
20 </table> | |
21 <br> | |
22 <p> | |
23 | |
24 | |
25 <!--END OF HEADER--> | |
26 | |
27 | |
28 | |
29 | |
30 | |
31 | |
32 <H2> Function </H2> | |
33 Calculate the P2 index of each gene | |
34 <!-- | |
35 DON'T WRITE ANYTHING HERE. | |
36 IT IS DONE FOR YOU. | |
37 --> | |
38 | |
39 | |
40 | |
41 | |
42 <H2>Description</H2> | |
43 <p> | |
44 gp2 calculates the P2 index for each gene. This index describes<br /> | |
45 the proportion of codons conforming to the intermediate strength of<br /> | |
46 codon-anticodon interaction energy rule of Grosjean and Fiers:<br /> | |
47 P2 = (WWC+SSU)/(WWY+SSY) where W = A or U, S = C or G, and Y = C or U.<br /> | |
48 It indicates the efficiency of the codon-anticodon interaction, and has<br /> | |
49 been used as an indicator of the presence of translational selection.<br /> | |
50 <br /> | |
51 G-language SOAP service is provided by the<br /> | |
52 Institute for Advanced Biosciences, Keio University.<br /> | |
53 The original web service is located at the following URL:<br /> | |
54 <br /> | |
55 http://www.g-language.org/wiki/soap<br /> | |
56 <br /> | |
57 WSDL(RPC/Encoded) file is located at:<br /> | |
58 <br /> | |
59 http://soap.g-language.org/g-language.wsdl<br /> | |
60 <br /> | |
61 Documentation on G-language Genome Analysis Environment methods are<br /> | |
62 provided at the Document Center<br /> | |
63 <br /> | |
64 http://ws.g-language.org/gdoc/<br /> | |
65 <br /> | |
66 | |
67 </p> | |
68 | |
69 <H2>Usage</H2> | |
70 | |
71 Here is a sample session with gp2 | |
72 | |
73 <table width="90%"><tr><td bgcolor="#CCFFFF"><pre> | |
74 | |
75 % gp2 refseqn:NC_000913 | |
76 Calculate the P2 index of each gene | |
77 Codon usage output file [nc_000913.gp2]: | |
78 | |
79 </pre></td></tr></table> | |
80 | |
81 Go to the <a href="#input">input files</a> for this example<br> | |
82 Go to the <a href="#output">output files</a> for this example<br><br> | |
83 | |
84 <h2>Command line arguments</h2> | |
85 | |
86 <table border cellspacing=0 cellpadding=3 bgcolor="#ccccff"> | |
87 <tr bgcolor="#FFFFCC"> | |
88 <th align="left">Qualifier</th> | |
89 <th align="left">Type</th> | |
90 <th align="left">Description</th> | |
91 <th align="left">Allowed values</th> | |
92 <th align="left">Default</th> | |
93 </tr> | |
94 | |
95 <tr bgcolor="#FFFFCC"> | |
96 <th align="left" colspan=5>Standard (Mandatory) qualifiers</th> | |
97 </tr> | |
98 | |
99 <tr bgcolor="#FFFFCC"> | |
100 <td>[-sequence]<br>(Parameter 1)</td> | |
101 <td>seqall</td> | |
102 <td>Nucleotide sequence(s) filename and optional format, or reference (input USA)</td> | |
103 <td>Readable sequence(s)</td> | |
104 <td><b>Required</b></td> | |
105 </tr> | |
106 | |
107 <tr bgcolor="#FFFFCC"> | |
108 <td>[-outfile]<br>(Parameter 2)</td> | |
109 <td>outfile</td> | |
110 <td>Codon usage output file</td> | |
111 <td>Output file</td> | |
112 <td><i><*></i>.gp2</td> | |
113 </tr> | |
114 | |
115 <tr bgcolor="#FFFFCC"> | |
116 <th align="left" colspan=5>Additional (Optional) qualifiers</th> | |
117 </tr> | |
118 | |
119 <tr> | |
120 <td colspan=5>(none)</td> | |
121 </tr> | |
122 | |
123 <tr bgcolor="#FFFFCC"> | |
124 <th align="left" colspan=5>Advanced (Unprompted) qualifiers</th> | |
125 </tr> | |
126 | |
127 <tr bgcolor="#FFFFCC"> | |
128 <td>-[no]accid</td> | |
129 <td>boolean</td> | |
130 <td>Include to use sequence accession ID as query</td> | |
131 <td>Boolean value Yes/No</td> | |
132 <td>Yes</td> | |
133 </tr> | |
134 | |
135 </table> | |
136 | |
137 | |
138 <h2 id="input">Input file format</h2> | |
139 | |
140 <p> | |
141 The database definitions for following commands are available at<br /> | |
142 http://soap.g-language.org/kbws/embossrc<br /> | |
143 <br /> | |
144 gp2 reads one or more nucleotide sequences.<br /> | |
145 <br /> | |
146 | |
147 </p> | |
148 | |
149 <h2 id="output">Output file format</h2> | |
150 | |
151 <p> | |
152 The output from gp2 is to a plain text file.<br /> | |
153 <br /> | |
154 File: nc_000913.gp2<br /> | |
155 <br /> | |
156 <table width="90%"><tr><td bgcolor="#CCFFCC"> | |
157 Sequence: NC_000913<br /> | |
158 P2,gene<br /> | |
159 0.4444,thrL<br /> | |
160 0.4234,thrA<br /> | |
161 0.4565,thrB<br /> | |
162 0.5156,thrC<br /> | |
163 0.4074,yaaX<br /> | |
164 0.4494,yaaA<br /> | |
165 0.3621,yaaJ<br /> | |
166 0.6832,talB<br /> | |
167 <br /> | |
168 <font color=red>[Part of this file has been deleted for brevity]</font><br /> | |
169 <br /> | |
170 0.3692,yjjX<br /> | |
171 0.4912,ytjC<br /> | |
172 0.4271,rob<br /> | |
173 0.4318,creA<br /> | |
174 0.3065,creB<br /> | |
175 0.3851,creC<br /> | |
176 0.4320,creD<br /> | |
177 0.6395,arcA<br /> | |
178 0.7857,yjjY<br /> | |
179 0.3333,yjtD<br /> | |
180 </td></tr></table> | |
181 | |
182 </p> | |
183 | |
184 <h2>Data files</h2> | |
185 | |
186 <p> | |
187 None. | |
188 </p> | |
189 | |
190 <h2>Notes</h2> | |
191 | |
192 <p> | |
193 None. | |
194 </p> | |
195 | |
196 <h2>References</h2> | |
197 | |
198 <pre> | |
199 Gouy M, Gautier C. (1982) Codon usage in bacteria: correlation with gene | |
200 expressivity, Nucleic Acids Res, 10(22):7055-74. | |
201 | |
202 Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and | |
203 Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench | |
204 for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. | |
205 | |
206 Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for | |
207 large-scale analysis of high-throughput omics data, J. Pest Sci., | |
208 31, 7. | |
209 | |
210 Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome | |
211 Analysis Environment with REST and SOAP Web Service Interfaces, | |
212 Nucleic Acids Res., 38, W700-W705. | |
213 | |
214 </pre> | |
215 | |
216 <h2>Warnings</h2> | |
217 | |
218 <p> | |
219 None. | |
220 </p> | |
221 | |
222 <h2>Diagnostic Error Messages</h2> | |
223 | |
224 <p> | |
225 None. | |
226 </p> | |
227 | |
228 <h2>Exit status</h2> | |
229 | |
230 <p> | |
231 It always exits with a status of 0. | |
232 </p> | |
233 | |
234 <h2>Known bugs</h2> | |
235 | |
236 <p> | |
237 None. | |
238 </p> | |
239 | |
240 <h2>See also</h2> | |
241 | |
242 <table border cellpadding=4 bgcolor="#FFFFF0"><tr><th>Program name</th> | |
243 <th>Description</th></tr> | |
244 | |
245 <tr> | |
246 <td><a href="gcai.html">gcai</a></td> | |
247 <td>Calculate codon adaptation index for each gene</td> | |
248 </tr><tr> | |
249 <td><a href="gphx.html">gphx</a></td> | |
250 <td>Identify predicted highly expressed gene</td> | |
251 </tr> | |
252 | |
253 </table> | |
254 | |
255 <h2>Author(s)</h2> | |
256 | |
257 <pre> | |
258 Hidetoshi Itaya (celery@g-language.org) | |
259 Institute for Advanced Biosciences, Keio University | |
260 252-0882 Japan | |
261 | |
262 Kazuharu Arakawa (gaou@sfc.keio.ac.jp) | |
263 Institute for Advanced Biosciences, Keio University | |
264 252-0882 Japan</pre> | |
265 | |
266 <h2>History</h2> | |
267 | |
268 2012 - Written by Hidetoshi Itaya | |
269 | |
270 <h2>Target users</h2> | |
271 | |
272 This program is intended to be used by everyone and everything, from | |
273 naive users to embedded scrips. | |
274 | |
275 <h2>Comments</h2> | |
276 | |
277 None. | |
278 | |
279 </BODY> | |
280 </HTML> |