Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/gaaui.txt @ 0:8300eb051bea draft
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author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/gaaui.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,208 @@ + gaaui +Function + + Calculates various indece of amino acid usage + +Description + + gaaui calculates amino acid usage indices for proteins (excluding + formylmethionine). Calculated indices are as follows, + Laa: Length in amino acids + ndaa: Number of different amino acids + Haau: Entropy of amino acid usage + mmw: Mean molecular weight + gravy: Mean hydropathic indices of each amino acid + aroma: Relative frequency of aromatic amino acids + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with gaaui + +% gaaui refseqn:NC_000913 +Calculates various indece of amino acid usage +AAINDEX entry output file [nc_000913.gaaui]: + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + + Standard (Mandatory) qualifiers: + [-sequence] seqall Nucleotide sequence(s) filename and optional + format, or reference (input USA) + [-outfile] outfile [*.gaaui] AAINDEX entry output file + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: + -[no]accid boolean [Y] Include to use sequence accession ID as + query + + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-outfile" associated qualifiers + -odirectory2 string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + gaaui reads one or more nucleotide sequences. + +Output file format + + The output from gaaui is to a plain text file. + + File: nc_000913.gaaui + +Sequence: NC_000913 +Laa,ndaa,Haau,mmw,gravy,aroma,gene +20,8,2.4842,117.48,+0.0150,0.0000,thrL +819,20,4.0887,126.65,+0.0328,0.0659,thrA +309,20,4.1228,126.35,+0.0181,0.0712,thrB +427,20,4.0806,128.00,-0.1014,0.0843,thrC +97,18,3.9165,133.54,-1.0268,0.0928,yaaX +257,19,4.0733,132.55,-0.4117,0.1089,yaaA +475,20,4.0413,126.46,+0.6781,0.1242,yaaJ +316,20,4.0395,128.99,-0.2165,0.0728,talB + + [Part of this file has been deleted for brevity] + +169,20,4.0001,124.90,+0.0231,0.0710,yjjX +214,20,3.9937,129.77,-0.3813,0.0374,ytjC +288,20,4.1421,132.58,-0.3628,0.1111,rob +156,20,4.0627,126.72,-0.0442,0.0705,creA +228,20,4.0471,131.94,-0.1408,0.0789,creB +473,20,4.0254,128.01,+0.0023,0.0677,creC +449,20,4.0871,128.66,+0.2082,0.0980,creD +237,20,4.0729,132.54,-0.4970,0.0675,arcA +45,15,3.5800,123.27,+0.7533,0.0222,yjjY +227,20,4.0283,128.63,-0.0031,0.0573,yjtD + + +Data files + + None. + +Notes + + None. + +References + + Lobry, JR. and Gautier, C. (1994) Hydrophobicity, expressivity and + aromaticity are the major trends of amino-acid usage in 999 Escherichia + coli chromosome-encoded genes, Nucleic Acids Res, 22:3174-3180.a + + Zavala A et al. (2002) Trends in codon and amino acid usage in Thermotoga + maritima J Mol Evol. 54(5):563-8. + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + gaminoinfo Prints out basic amino acid sequence statistics + gbui Calculates base usage indeces for protein-codin sequences + gcodoncompiler Calculates various kinds of amino acid and codon usage data + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +