Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/gcodoncompiler.txt @ 0:8300eb051bea draft
Initial upload
author | ktnyt |
---|---|
date | Fri, 26 Jun 2015 05:19:29 -0400 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/gcodoncompiler.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,213 @@ + gcodoncompiler +Function + + Calculate various kinds of amino acid and codon usage data + +Description + + gcodoncompiler calculates various kinds of amino acid and codon usage data. + The following values are calculable: + A0: Absolute amino acid frequency + A1: Relative amino acid frequency + C0: Absolute codon frequency + C1: Relative codon frequency in a complete sequence + C2: Relative codon frequency in each amino acid + C3: Relative synonymous codon usage + C4: Relative adaptiveness + C5: Maximum or minor codon + + For amino acids unpresent in a gene, C2-C3 does not calculate the values. + By using R* in place, such values are hypothesized that alternative + synonymous codons are used with equal frequency. + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with gcodoncompiler + +% gcodoncompiler refseqn:NC_000913 +Calculate various kinds of amino acid and codon usage data +Codon usage output file [nc_000913.gcodoncompiler]: + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + + Standard (Mandatory) qualifiers: + [-sequence] seqall Nucleotide sequence(s) filename and optional + format, or reference (input USA) + [-outfile] outfile [*.gcodoncompiler] Codon usage output file + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: + -translate boolean [N] Include to translate using standard + codon table + -startcodon boolean [N] Include to include start codon + -stopcodon boolean [N] Include to include stop codon + -delkey string [[^ACDEFGHIKLMNPQRSTVWYacgtU]] Regular + expression to delete key (i.e. amino acids + and nucleotides) (Any string) + -data menu [R0] Kinds of codon usage data. R* + hypothesizes amino acids which are not + present in the gene (Values: A0 (Absolute + amino acid frequency ('AA')); A1 (Relative + amino acid frequency ('RAAU')); C0 (Absolute + codon frequency ('AF')); C1 (Relative codon + frequency in a complete sequence); C2 + (Relative codon frequency in each amino acid + ('RF')); C3 (Relative synonymous codon + usage ('RSCU')); C4 (Relative adaptiveness); + i.e., ratio to maximum of minor codon ('W') + C5 (Maximum (1) or minor (0) codon); R0 + (Absolute codon frequency ('AF')); R1 + (Relative codon frequency in a complete + sequence); R2 (Relative codon frequency in + each amino acid ('RF')); R3 (Relative + synonymous codon usage ('RSCU')); R4 + (Relative adaptiveness); i.e., ratio to + maximum of minor codon ('W') R5 (Maximum (1) + or minor (0) codon)) + -[no]accid boolean [Y] Include to use sequence accession ID as + query + + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-outfile" associated qualifiers + -odirectory2 string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + gcodoncompiler reads one or more nucleotide sequences. + +Output file format + + The output from gcodoncompiler is to a plain text file. + + File: nc_000913.gcodoncompiler + +Sequence: NC_000913 +Agca,Agcc,Agcg,Agct,Ctgc,Ctgt,Dgac,Dgat,Egaa,Egag,Fttc,Fttt,Ggga,Gggc,Gggg,Gggt,Hcac,Hcat,Iata,Iatc,Iatt,Kaaa,Kaag,Lcta,Lctc,Lctg,Lctt,Ltta,Lttg,Matg,Naac,Naat,Pcca,Pccc,Pccg,Pcct,Qcaa,Qcag,Raga,Ragg,Rcga,Rcgc,Rcgg,Rcgt,Sagc,Sagt,Stca,Stcc,Stcg,Stct,Taca,Tacc,Tacg,Tact,Utga,Vgta,Vgtc,Vgtg,Vgtt,Wtgg,Ytac,Ytat,locus_tag +26551,33911,44924,20010,8486,6707,25234,42161,52362,23474,21841,29334,10226,39395,14472,32678,12830,16952,5356,33359,40221,44272,13398,5079,14709,70441,14410,18097,17936,32971,28329,22786,11063,7142,30994,9130,20216,38169,2495,1366,4529,29308,6991,27864,21132,11323,9159,11332,11759,10992,8979,31001,18989,11581,3,14337,20240,34499,24056,20071,16088,21069, + + +Data files + + None. + +Notes + + None. + +References + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + gaminoinfo Prints out basic amino acid sequence statistics + gaaui Calculates various indece of amino acid usage + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +