Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/greporiter.txt @ 0:8300eb051bea draft
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author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/greporiter.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,210 @@ + greporiter +Function + + Get the positions of replication origin and terminus + +Description + + greporiter returns the positions of replication origin and terminus + in bacterial genomes by several means. + + 1. Use of databases + By default, grep_ori_ter tries to retrieve the position of replication + origin in DoriC Gao and Zhang (2007) database, and the position of + replication terminus from the supplemental data provided in + Kono et al. (2011). + If the position of origin cannot be found in the database, but "rep_origin" + feature is available, center position within this feature is used for + origin. + + 2. Oriloc + Using -orilocoption, you can predict the replication origin and + terminus using the popular Oriloc program developed by Lobry et al. + available as part of the SeqinR package Frank and Lobry (2000). + + 3. Use GC skew shift-point + If the positions of origin or terminus cannot be found in the databases, + grep_ori_ter automatically calls find_ori_ter() method to predict the + positions using GC skew shift-points at one-base-pair resolution. + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with greporiter + +% greporiter refseqn:NC_000913 +Get the positions of replication origin and terminus +Output file [nc_000913.greporiter]: + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + + Standard (Mandatory) qualifiers: + [-sequence] seqall Nucleotide sequence(s) filename and optional + format, or reference (input USA) + [-outfile] outfile [*.greporiter] Output file name + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: + -oriloc boolean [N] Include Oriloc for prediction + -gcskew boolean [N] Include to use GC skew shift-point for + prediction + -difthreshold integer [0] Distance between the GC skew shift point + and predicted dif site expressed as the + precentage of genome size, used as a + threshold to retrieve dif sequence from the + database (Any integer value) + -dbonly boolean [N] Include to only use values available in + databases and to suppress prediction + -[no]accid boolean [Y] Include to use sequence accession ID as + query + + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-outfile" associated qualifiers + -odirectory2 string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + greporiter reads one or more nucleotide sequences. + +Output file format + + The output from greporiter is to a plain text file. + + File: nc_000913.greporiter + +Sequence: NC_000913 Origin: 3923881 Terminus: 1550412 + + +Data files + + None. + +Notes + + None. + +References + + Gao F and Zhang CT (2007) DoriC: a database of oriC regions in bacterial + genomes, Bioinformatics, 23(14):1866-1867 + + Kono N et al. (2011) Comprehensive prediction of chromosome dimer resolution + sites in bacterial genomes, BMC Genomics, 12(1):19 + + Frank AC and Lobry JR (2000) "Oriloc: prediction of replication boundaries + in unannotated bacterial chromosomes", Bioinformatics, 16(6):560-561 + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + gfindoriter Predicts the replication origin and terminus in bacterial + genomes + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +