Mercurial > repos > ktnyt > gembassy
diff GEMBASSY-1.0.3/doc/text/gshuffleseq.txt @ 0:8300eb051bea draft
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author | ktnyt |
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date | Fri, 26 Jun 2015 05:19:29 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEMBASSY-1.0.3/doc/text/gshuffleseq.txt Fri Jun 26 05:19:29 2015 -0400 @@ -0,0 +1,208 @@ + gshuffleseq +Function + + Create randomized sequence with conserved k-mer composition + +Description + + gshuffleseq shuffles and randomizes the given sequence, conserving the + nucleotide/peptide k-mer content of the original sequence. + + For k=1, i.e. shuffling sequencing preserving single nucleotide composition, + Fisher-Yates Algorithm is employed. + For k>1, shuffling preserves all k-mers (all k where k=1~k). For example, + k=3 preserves all triplet, doublet, and single nucleotide composition. + Algorithm for k-mer preserved shuffling is non-trivial, which is solved + by graph theoretical approach with Eulerian random walks in the graph of + k-1-mers. See Jiang et al., Kandel et al., and Propp et al., for details + of this algorithm. + + G-language SOAP service is provided by the + Institute for Advanced Biosciences, Keio University. + The original web service is located at the following URL: + + http://www.g-language.org/wiki/soap + + WSDL(RPC/Encoded) file is located at: + + http://soap.g-language.org/g-language.wsdl + + Documentation on G-language Genome Analysis Environment methods are + provided at the Document Center + + http://ws.g-language.org/gdoc/ + +Usage + +Here is a sample session with gshuffleseq + +% gshuffleseq tsw:hbb_human +Create randomized sequence with conserved k-mer composition +output sequence [hbb_human.fasta]: + + Go to the input files for this example + Go to the output files for this example + +Command line arguments + + Standard (Mandatory) qualifiers: + [-sequence] seqall Sequence(s) filename and optional format, or + reference (input USA) + [-outseq] seqout [<sequence>.<format>] Sequence filename and + optional format (output USA) + + Additional (Optional) qualifiers: (none) + Advanced (Unprompted) qualifiers: + -k integer [1] Sequence k-mer to preserve composition + (Any integer value) + + Associated qualifiers: + + "-sequence" associated qualifiers + -sbegin1 integer Start of each sequence to be used + -send1 integer End of each sequence to be used + -sreverse1 boolean Reverse (if DNA) + -sask1 boolean Ask for begin/end/reverse + -snucleotide1 boolean Sequence is nucleotide + -sprotein1 boolean Sequence is protein + -slower1 boolean Make lower case + -supper1 boolean Make upper case + -scircular1 boolean Sequence is circular + -sformat1 string Input sequence format + -iquery1 string Input query fields or ID list + -ioffset1 integer Input start position offset + -sdbname1 string Database name + -sid1 string Entryname + -ufo1 string UFO features + -fformat1 string Features format + -fopenfile1 string Features file name + + "-outseq" associated qualifiers + -osformat2 string Output seq format + -osextension2 string File name extension + -osname2 string Base file name + -osdirectory2 string Output directory + -osdbname2 string Database name to add + -ossingle2 boolean Separate file for each entry + -oufo2 string UFO features + -offormat2 string Features format + -ofname2 string Features file name + -ofdirectory2 string Output directory + + General qualifiers: + -auto boolean Turn off prompts + -stdout boolean Write first file to standard output + -filter boolean Read first file from standard input, write + first file to standard output + -options boolean Prompt for standard and additional values + -debug boolean Write debug output to program.dbg + -verbose boolean Report some/full command line options + -help boolean Report command line options and exit. More + information on associated and general + qualifiers can be found with -help -verbose + -warning boolean Report warnings + -error boolean Report errors + -fatal boolean Report fatal errors + -die boolean Report dying program messages + -version boolean Report version number and exit + +Input file format + + The database definitions for following commands are available at + http://soap.g-language.org/kbws/embossrc + + gshuffleseq reads one or more nucleotide or protein sequences. + +Output file format + + The output from gshuffleseq is to . + + File: hbb_human.fasta + +>HBB_HUMAN P68871 Hemoglobin subunit beta (Beta-globin) (Hemoglobin beta chain) (LVV-hemorphin-7) +KGWLDLVAGAAHFVRRLKMLLEVDWAAHEERVGTSNPNNALKNEAADVEVHSPTHVNPTQ +LVLVQVGFGTLHLQGVECPKPKPGGVALKPVAHLLAMKECTLVALGSDFYVDHGSDGEDK +GFKAYVLATSFFAYTNFLHGKVKHVLF + + +Data files + + None. + +Notes + + None. + +References + + Fisher R.A. and Yates F. (1938) "Example 12", Statistical Tables, London + + Durstenfeld R. (1964) "Algorithm 235: Random permutation", CACM 7(7):420 + + Jiang M., Anderson J., Gillespie J., and Mayne M. (2008) "uShuffle: + a useful tool for shuffling biological sequences while preserving the + k-let counts", BMC Bioinformatics 9:192 + + Kandel D., Matias Y., Unver R., and Winker P. (1996) "Shuffling biological + sequences", Discrete Applied Mathematics 71(1-3):171-185 + + Propp J.G. and Wilson D.B. (1998) "How to get a perfectly random sample + from a generic Markov chain and generate a random spanning tree of a + directed graph", Journal of Algorithms 27(2):170-217 + + Arakawa, K., Mori, K., Ikeda, K., Matsuzaki, T., Konayashi, Y., and + Tomita, M. (2003) G-language Genome Analysis Environment: A Workbench + for Nucleotide Sequence Data Mining, Bioinformatics, 19, 305-306. + + Arakawa, K. and Tomita, M. (2006) G-language System as a Platform for + large-scale analysis of high-throughput omics data, J. Pest Sci., + 31, 7. + + Arakawa, K., Kido, N., Oshita, K., Tomita, M. (2010) G-language Genome + Analysis Environment with REST and SOAP Web Service Interfaces, + Nucleic Acids Res., 38, W700-W705. + +Warnings + + None. + +Diagnostic Error Messages + + None. + +Exit status + + It always exits with a status of 0. + +Known bugs + + None. + +See also + + shuffleseq Shuffles a set of sequences maintaining composition + +Author(s) + + Hidetoshi Itaya (celery@g-language.org) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + + Kazuharu Arakawa (gaou@sfc.keio.ac.jp) + Institute for Advanced Biosciences, Keio University + 252-0882 Japan + +History + + 2012 - Written by Hidetoshi Itaya + 2013 - Fixed by Hidetoshi Itaya + +Target users + + This program is intended to be used by everyone and everything, from + naive users to embedded scripts. + +Comments + + None. +