view GEMBASSY-1.0.3/src/ggcwin.c @ 1:84a17b3fad1f draft

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/******************************************************************************
** @source ggcwin
**
** Calculates the GC content along the given genome
**
** @author Copyright (C) 2012 Hidetoshi Itaya
** @version 1.0.3
** @modified 2012/1/20  Hidetoshi Itaya  Created!
** @modified 2013/6/16  Revision 1
** @modified 2015/2/7   RESTify
** @modified 2015/2/7   Refactor
** @@
**
** This program is free software; you can redistribute it and/or
** modify it under the terms of the GNU General Public License
** as published by the Free Software Foundation; either version 2
** of the License, or (at your option) any later version.
**
** This program is distributed in the hope that it will be useful,
** but WITHOUT ANY WARRANTY; without even the implied warranty of
** MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
** GNU General Public License for more details.
**
** You should have received a copy of the GNU General Public License
** along with this program; if not, write to the Free Software
** Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA  02111-1307, USA.
******************************************************************************/

#include "emboss.h"
#include "glibs.h"




/* @prog ggcwin ***************************************************************
**
** Calculates the GC content along the given genome
**
******************************************************************************/

int main(int argc, char *argv[])
{
  embInitPV("ggcwin", argc, argv, "GEMBASSY", "1.0.3");

  AjPSeqall seqall;
  AjPSeq    seq;
  AjPStr    inseq      = NULL;

  AjBool accid  = ajFalse;
  AjPStr restid = NULL;
  AjPStr seqid  = NULL;

  AjPStr base = NULL;
  AjPStr url  = NULL;

  AjPStr    tmpname = NULL;
  AjPSeqout tmpout  = NULL;

  ajint  window = 0;
  AjBool at     = 0;
  AjBool purine = 0;
  AjBool keto   = 0;

  AjBool      plot = 0;
  AjPFile     outf = NULL;
  AjPFilebuff buff = NULL;
  AjPGraph    mult = NULL;

  gPlotParams gpp;
  AjPStr      title = NULL;

  seqall = ajAcdGetSeqall("sequence");
  window = ajAcdGetInt("window");
  at     = ajAcdGetBoolean("at");
  purine = ajAcdGetBoolean("purine");
  keto   = ajAcdGetBoolean("keto");

  plot = ajAcdGetToggle("plot");
  outf = ajAcdGetOutfile("outfile");
  mult = ajAcdGetGraphxy("graph");

  base = ajStrNewC("rest.g-language.org");

  gAssignUniqueName(&tmpname);
  ajStrAppendC(&tmpname, ".fasta");

  while(ajSeqallNext(seqall, &seq))
    {
      tmpout = ajSeqoutNew();

      if(!ajSeqoutOpenFilename(tmpout, tmpname))
        {
          embExitBad();
        }

      ajSeqoutSetFormatS(tmpout,ajStrNewC("fasta"));
      ajSeqoutWriteSeq(tmpout, seq);
      ajSeqoutClose(tmpout);
      ajSeqoutDel(&tmpout);

      ajFmtPrintS(&url, "http://%S/upload/upl.pl", base);
      gFilePostSS(url, tmpname, &restid);
      ajStrDel(&url);
      ajSysFileUnlinkS(tmpname);

      ajStrAssignS(&seqid, ajSeqGetAccS(seq));

      if(ajStrGetLen(seqid) == 0)
        {
          ajStrAssignS(&seqid, ajSeqGetNameS(seq));
        }

      if(ajStrGetLen(seqid) == 0)
        {
          ajWarn("No valid header information\n");
        }

      url = ajStrNew();

      ajFmtPrintS(&url, "http://%S/%S/gcwin/window=%d/at=%d/purine=%d/"
                  "keto=%d/output=f/", base, restid, window, at, purine, keto);

      if(plot)
        {
          title = ajStrNew();

          ajStrAppendC(&title, argv[0]);
          ajStrAppendC(&title, " of ");
          ajStrAppendS(&title, seqid);

          gpp.title = ajStrNewS(title);
          gpp.xlab = ajStrNewC("location");
          gpp.ylab = ajStrNewC("GC content");

          if(!gFilebuffURLS(url, &buff))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }

          if(!gPlotFilebuff(buff, mult, &gpp))
            {
              ajDie("Error in plotting\n");
            }

          AJFREE(gpp.title);
          AJFREE(gpp.xlab);
          AJFREE(gpp.ylab);
          ajStrDel(&title);
          ajFilebuffDel(&buff);
        }
      else
        {
          ajFmtPrintF(outf, "Sequence: %S\n", seqid);
          if(!gFileOutURLS(url, &outf))
            {
              ajDie("File downloading error from:\n%S\n", url);
            }
        }
      ajStrDel(&url);
      ajStrDel(&restid);
      ajStrDel(&seqid);
    }

  ajFileClose(&outf);

  ajSeqallDel(&seqall);
  ajSeqDel(&seq);
  ajStrDel(&base);

  embExit();

  return 0;
}