view abims_sartools_deseq2_wrapper.py @ 4:05c9b1a7f44e draft default tip

Uploaded new release 1.7.3

#abims_sartools_deseq2_wrapper.py
#Author: Loraine Gueguen
# imports
import os, argparse



def main():

    print("Start of galaxy wrapper")

    #Check R and Rscript are installed
    check_r_cmd="command -v R >/dev/null 2>&1 || { echo >&2 'This tool requires R but it is not installed.  Aborting.'; exit 1; }"
    check_rscript_cmd="command -v Rscript >/dev/null 2>&1 || { echo >&2 'This tool requires Rscript but it is not installed.  Aborting.'; exit 1; }"
    os.system(check_r_cmd)
    os.system(check_rscript_cmd)

    #Get arguments
    parser = argparse.ArgumentParser()
    parser.add_argument('--projectName')
    parser.add_argument('--author')
    parser.add_argument('--targetFile')
    parser.add_argument('--rawDir')
    parser.add_argument('--featuresToRemove')
    parser.add_argument('--varInt')
    parser.add_argument('--condRef')
    parser.add_argument('--batch')
    parser.add_argument('--fitType')
    parser.add_argument('--cooksCutoff')
    parser.add_argument('--independentFiltering')
    parser.add_argument('--alpha')
    parser.add_argument('--pAdjustMethod')
    parser.add_argument('--typeTrans')
    parser.add_argument('--locfunc')
    parser.add_argument('--colors')
    parser.add_argument('--forceCairoGraph')
    parser.add_argument('--figures_html')
    parser.add_argument('--figures_html_files_path')
    parser.add_argument('--tables_html')
    parser.add_argument('--tables_html_files_path')
    parser.add_argument('--rdata')
    parser.add_argument('--report_html')
    parser.add_argument('--log')
    args = parser.parse_args()
    projectName=args.projectName
    author=args.author
    targetFile=args.targetFile
    rawDir=args.rawDir
    featuresToRemove=args.featuresToRemove
    varInt=args.varInt
    condRef=args.condRef
    batch=args.batch
    fitType=args.fitType
    cooksCutoff=args.cooksCutoff
    independentFiltering=args.independentFiltering
    alpha=args.alpha
    pAdjustMethod=args.pAdjustMethod
    typeTrans=args.typeTrans
    locfunc=args.locfunc
    colors=args.colors
    forceCairoGraph=args.forceCairoGraph
    figures_html=args.figures_html
    figures_html_files_path=args.figures_html_files_path
    tables_html=args.tables_html
    tables_html_files_path=args.tables_html_files_path
    rdata=args.rdata
    report_html=args.report_html
    log=args.log
    #Print the parameters selected
    print("Wrapper arguments: %s" %(args))

    #Get the working directory path
    working_directory = os.getcwd()
    #Get the script directory path
    script_directory=os.path.dirname(os.path.realpath(__file__))
 
    #Unzip files from rawDir
    rawDir_unzipped_path=working_directory+"/rawDir_unzipped"
    os.mkdir(rawDir_unzipped_path)
    unzip_cmd="unzip -j %s -d %s" % (rawDir,rawDir_unzipped_path) #-j arg: junk paths
    os.system(unzip_cmd)

    #Create the command
    cmd="Rscript --no-save --no-restore %s/template_script_DESeq2_CL.r --projectName %s --author %s " % (script_directory,projectName,author)
    cmd+="--targetFile %s --rawDir %s --featuresToRemove %s --varInt %s --condRef %s " % (targetFile,rawDir_unzipped_path,featuresToRemove,varInt,condRef)
    if batch and batch!="NULL":
        cmd+="--batch %s " % (batch)
    if fitType:
        cmd+="--fitType %s " % (fitType)
    if cooksCutoff:
        cmd+="--cooksCutoff %s " % (cooksCutoff)
    if independentFiltering:
        cmd+="--independentFiltering %s " % (independentFiltering)
    if alpha:
        cmd+="--alpha %s " % (alpha)
    if pAdjustMethod:
        cmd+="--pAdjustMethod %s " % (pAdjustMethod)
    if typeTrans:
        cmd+="--typeTrans %s " % (typeTrans)
    if locfunc:
        cmd+="--locfunc %s " % (locfunc)
    if colors:
        cmd+="--colors %s " % (colors)
    if forceCairoGraph:
        cmd+="--forceCairoGraph %s " % (forceCairoGraph)
    cmd+="> %s 2>&1" % (log)
    print("Rscript command: %s" % (cmd))
    os.system(cmd)

    #Get output files
    os.mkdir(figures_html_files_path)
    os.mkdir(tables_html_files_path)
    rsync_figures_dir_cmd="rsync -r figures/* %s/." % (figures_html_files_path)
    rsync_tables_dir_cmd="rsync -r tables/* %s/." % (tables_html_files_path)
    os.system(rsync_figures_dir_cmd)
    os.system(rsync_tables_dir_cmd)
    figures_html_create_cmd="python %s/make_html.py --tool SARTools_DESeq2 --output_type Figures --output_dir %s --output_html %s" % (script_directory,figures_html_files_path,figures_html)
    tables_html_create_cmd="python %s/make_html.py --tool SARTools_DESeq2 --output_type Tables --output_dir %s --output_html %s" % (script_directory,tables_html_files_path,tables_html)
    os.system(figures_html_create_cmd)
    os.system(tables_html_create_cmd)
    rsync_rdata_file_cmd="rsync %s.RData %s" % (projectName,rdata)
    rsync_report_file_cmd="rsync %s_report.html %s" % (projectName,report_html)
    os.system(rsync_rdata_file_cmd)
    os.system(rsync_report_file_cmd)

    print("End of galaxy wrapper")

if __name__ == '__main__':
    main()