Mercurial > repos > lldelisle > incucyte_stack_and_upload_omero
changeset 1:3c942429f610 draft default tip
planemo upload for repository https://github.com/lldelisle/tools-lldelisle/tree/master/tools/incucyte_stack_and_upload_omero commit de447190f0743b7f6a3b85e13f712fc07682af3c
author | lldelisle |
---|---|
date | Thu, 21 Dec 2023 17:04:46 +0000 |
parents | e1cba36becb2 |
children | |
files | incucyte_stack_and_upload_omero.xml stack_buildXml.groovy |
diffstat | 2 files changed, 14 insertions(+), 9 deletions(-) [+] |
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--- a/incucyte_stack_and_upload_omero.xml Tue Dec 19 15:02:41 2023 +0000 +++ b/incucyte_stack_and_upload_omero.xml Thu Dec 21 17:04:46 2023 +0000 @@ -1,7 +1,7 @@ <tool id="incucyte_stack_and_upload_omero" name="Stack Incucyte Plate" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="20.01" license="BSD-3"> <description>And upload to omero</description> <macros> - <token name="@TOOL_VERSION@">20231219</token> + <token name="@TOOL_VERSION@">20231221</token> <token name="@VERSION_SUFFIX@">0</token> </macros> <requirements>
--- a/stack_buildXml.groovy Tue Dec 19 15:02:41 2023 +0000 +++ b/stack_buildXml.groovy Thu Dec 21 17:04:46 2023 +0000 @@ -26,7 +26,7 @@ * and Lucille Delisle, EPFL - SV - UPDUB * and Pierre Osteil, EPFL - SV - UPDUB * - * Last modification: 2023-12-19 + * Last modification: 2023-12-21 * * = COPYRIGHT = * © All rights reserved. ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE, Switzerland, BioImaging And Optics Platform (BIOP), 2023 @@ -120,7 +120,7 @@ LETTERS = new String("ABCDEFGHIJKLMNOP") // Version number = date of last modif -VERSION = "20231219" +VERSION = "20231221" /** Key-Value pairs namespace */ GENERAL_ANNOTATION_NAMESPACE = "openmicroscopy.org/omero/client/mapAnnotation" @@ -322,13 +322,15 @@ first_image_infos = Opener.getTiffFileInfo(files_matching[0].getAbsolutePath()) // We define the imageplus object ImagePlus single_channel_imp + ImageStack stack if (first_image_infos == null) { // They are raw from incucyte // We need to open images one by one and add them to the stack - ImageStack stack = new ImageStack(first_imp.width, first_imp.height); + stack = new ImageStack(first_imp.width, first_imp.height); files_matching.each{ ImagePlus single_imp = (new Opener()).openUsingBioFormats(it.getAbsolutePath()) - String new_title = single_imp.getTitle().split(" - ")[0] + String new_title = it.getName() + // println "Title of slice: " + new_title stack.addSlice(new_title, single_imp.getProcessor()) } single_channel_imp = new ImagePlus(FilenameUtils.getBaseName(folder_list.get(i).getAbsolutePath()), stack); @@ -340,7 +342,6 @@ // Phase are 8-bit and need to be changed to 16-bit // Other are already 16-bit but it does not hurt IJ.run(single_channel_imp, "16-bit", "") - // check frame size if (nT == 0) { // This is the first channel with images @@ -348,7 +349,11 @@ first_channel = channels_list.get(i) // Process all dates: Pattern date_pattern = Pattern.compile(REGEX_FOR_DATE) - ImageStack stack = single_channel_imp.getStack() + // The IJ.run 16-bit seems to remove the name of the stack + // When there is only one stack + if (nT !=1 || stack == null) { + stack = single_channel_imp.getStack() + } // Go to the first time (which is slice) single_channel_imp.setSlice(1) int currentSlice = single_channel_imp.getCurrentSlice() @@ -844,7 +849,7 @@ // Returns a date from a label and a date_pattern def getDate(String label, Pattern date_pattern){ -// println "Trying to get date from " + label + // println "Trying to get date from " + label Matcher date_m = date_pattern.matcher(label) LocalDateTime dateTime if (date_m.matches()) { @@ -854,7 +859,7 @@ dateTime = LocalDateTime.parse("1970-01-" + 1 + (date_m.group(1) as int) + "T" + date_m.group(2) + ":" + date_m.group(3)) } } -// println "Found " + dateTime + // println "Found " + dateTime return dateTime }