view revertR2orientationInBam.xml @ 1:21ddefab2e4f draft default tip

planemo upload for repository https://github.com/lldelisle/tools-lldelisle/tree/master/tools/revertR2orientationInBam commit 49a42d0e75d6ae7faa3ce399524157c35546fbaa

<tool id="revertR2orientationInBam" name="revertR2orientationInBam" version="0.0.2" profile="16.04">
  <description> Revert the mapped orientation of R2 mates in a bam.</description>
  <requirements>
    <requirement type="package" version="1.16.1">samtools</requirement>
    <requirement type="package" version="5.1.0">gawk</requirement>
  </requirements>
  <stdio>
    <!-- Anything other than zero is an error -->
    <exit_code range="1:" />
    <exit_code range=":-1" />
    <!-- In case the return code has not been set propery check stderr too -->
    <regex match="Error:" />
    <regex match="Exception:" />
  </stdio>
  <command>
<![CDATA[
        set -o pipefail;
        bash $__tool_directory__/revertR2orientationInBam.sh '$input' '$output'
]]>
  </command>
  <inputs>
    <!-- note unsorted bam includes all derived bam types (inlcuding bam which is sorted) -->
    <param name="input" type="data" format="sam,bam" label="Select a sam or bam file."/>
  </inputs>
  <outputs>
    <data format="bam" name="output" label="$input.name with R2 orientation reversed"/>
  </outputs>

  <tests>
    <test>
      <param name="input" value="input.sam"/>
      <output name="output" file="output.bam" ftype="bam" lines_diff="2"/>
    </test>
  </tests>  
  <help>
<![CDATA[
    This tool is very useful when you have paired-end stranded RNA-seq.
    Using this tool prior to a bedtools genome coverage allow to have strand specific coverage using both mates.
    It uses samtools to convert input to sam format and then awk to modify the flag "reverse strand" for the second mate of pairs.
]]>
  </help>
</tool>