view fastq-join.xml @ 0:e6f1c31279db draft

Initial version with fastq-join

<tool id="fastq_join" name="fastq-join" version="0.1">
    <description> - Joins two paired-end reads on the overlapping ends</description>
    <requirements>
        <requirement type="package" version="1.1.2-469">ea-utils</requirement>
    </requirements>
    <command>
        fastq-join 
        -v '$splitChar'
        -p $pctMaxDiff
        -m $minOverlap
        #if $stitchLengthReport:
        -r $outputStitchLengthReport
        #end if
        $read1
        $read2
        -o $outputUnmatched1 -o $outputUnmatched2 -o $outputJoined 
    </command>
    <inputs>
        <param format="fastq, fastqillumina, fastqsanger, fastqsolexa" name="read1" type="data" label="Read 1 Fastq" />
        <param format="fastq, fastqillumina, fastqsanger, fastqsolexa" name="read2" type="data" label="Read 2 Fastq" />
        <param name="splitChar" type="text" value=" " label="Split read ids on this character" help="Default is space ' ' for Illumina reads" />
        <param name="pctMaxDiff" type="float" value="8" min="0" max="100" label="Maximum percentage difference between matching segments" />
        <param name="minOverlap" type="integer" value="6" min="1" label="Minimum length of matching segements" />
        <param name="stitchLengthReport" type="boolean" value="False" label="Output verbose stitch length report" />
    </inputs>

    <outputs>
        <data format="input" format_source="read1" name="outputJoined" label="${tool.name} on ${on_string} (joined)"/>
        <data format="input" format_source="read1" name="outputUnmatched1" label="${tool.name} on ${on_string} (unmatched1)"/>
        <data format="input" format_source="read2" name="outputUnmatched2" label="${tool.name} on ${on_string} (unmatched2)"/>
        <data format="tabular" name="outputStitchLengthReport" label="${tool.name} on ${on_string} (stitch length report)">
            <filter>stitchLengthReport</filter>
        </data>
    </outputs>

    <stdio>
        <exit_code range="1:" level="fatal" description="Unknown error occurred" />
    </stdio>

    <tests>
        <test>
            <param name="read1" value="test_read1.fastq" />
            <param name="read2" value="test_read3.fastq" />
            <output name="outputJoined" file="testout.join.fastq" />
            <output name="outputUnmatched1" file="testout.un1.fastq" />
            <output name="outputUnmatched2" file="testout.un2.fastq" />
        </test>
    </tests>

    <help>
Overview
--------
fastq-join joins two paired-end reads on the overlapping ends.

Split read ids character: Verifies that the 2 files probe id's match up to char C. Use ' ' for Illumina reads.

Maximum difference is the maximum allowed percentage of bases that differ in the matching region.

Minimum overlap is the minimum number of bases that must overlap (with no more than the maximum difference) for reads to be joined.

Verbose stitch length report is a report for each joined paired of reads showing how large the overlapping section was.

This tool uses sqr(distance)/len for anchored alignment quality algorithm. It's a good measure of anchored alignment quality, akin to squared-deviation for means.  This tool uses the fastq-join program that is part of the ea-utils suite. See http://code.google.com/p/ea-utils/wiki/FastqJoin for details.
    </help>
</tool>