# HG changeset patch # User lparsons # Date 1447436594 18000 # Node ID 32304398ef671252c1cfbfc4caf784c0f000dd97 # Parent b7b3d008e2d33fa81db7bf7e08b63b74571c830f planemo upload for repository https://github.com/lparsons/galaxy_tools/tree/master/tools/fastx_barcode_splitter_enhanced commit c3b0be52079831f6ae58ff64e42d8ff54ce501e4 diff -r b7b3d008e2d3 -r 32304398ef67 fastx_barcode_splitter.xml --- a/fastx_barcode_splitter.xml Tue Oct 27 11:47:52 2015 -0400 +++ b/fastx_barcode_splitter.xml Fri Nov 13 12:43:14 2015 -0500 @@ -1,7 +1,7 @@ - + - + + + @@ -50,7 +52,13 @@ - + + + + + + + @@ -85,7 +93,7 @@ - + @misc{gordon_fastx-toolkit_????, title = {{FASTX}-{Toolkit}}, url = {http://hannonlab.cshl.edu/fastx_toolkit/index.html}, diff -r b7b3d008e2d3 -r 32304398ef67 fastx_barcode_splitter_galaxy_wrapper.sh --- a/fastx_barcode_splitter_galaxy_wrapper.sh Tue Oct 27 11:47:52 2015 -0400 +++ b/fastx_barcode_splitter_galaxy_wrapper.sh Fri Nov 13 12:43:14 2015 -0500 @@ -19,7 +19,8 @@ # Modified by Lance Parsons (lparsons@princeton.edu) # 2011-03-15 Adapted to allow galaxy to determine filetype # 2015-10-21 Updated to make compatible with OSX (BSD sed) -# +# 2015-11-13 Removed LIBRARY_NAME, no longer needed + #This is a shell script wrapper for 'fastx_barcode_splitter.pl' # # 1. Output files are saved at the dataset's files_path directory. @@ -29,28 +30,22 @@ # (so lazy users can just click on the URLs and get their files) if [ "$1x" = "x" ]; then - echo "Usage: $0 [BARCODE FILE] [FASTQ FILE] [LIBRARY_NAME] [OUTPUT_PATH] [FILETYPE]" >&2 + echo "Usage: $0 [BARCODE FILE] [FASTQ FILE] [OUTPUT_PATH] [FILETYPE]" >&2 exit 1 fi BARCODE_FILE="$1" FASTQ_FILE="$2" -LIBNAME="$3" -OUTPUT_PATH="$4" -FILETYPE="$5" -shift 5 +OUTPUT_PATH="$3" +FILETYPE="$4" +shift 4 # The rest of the parameters are passed to the split program if [ "${OUTPUT_PATH}x" = "x" ]; then - echo "Usage: $0 [BARCODE FILE] [FASTQ FILE] [LIBRARY_NAME] [OUTPUT_PATH] [FILETYPE]" >&2 + echo "Usage: $0 [BARCODE FILE] [FASTQ FILE] [OUTPUT_PATH] [FILETYPE]" >&2 exit 1 fi -#Sanitize library name, make sure we can create a file with this name -LIBNAME=${LIBNAME%.gz} -LIBNAME=${LIBNAME%.txt} -LIBNAME=$(echo "$LIBNAME" | tr -cd '[:alnum:]_') - if [ ! -r "$FASTQ_FILE" ]; then echo "Error: Input file ($FASTQ_FILE) not found!" >&2 exit 1 @@ -65,24 +60,20 @@ exit 1 fi -PUBLICURL="" BASEPATH="$OUTPUT_PATH/" -#PREFIX="$BASEPATH"`date "+%Y-%m-%d_%H%M__"`"${LIBNAME}__" -PREFIX="$BASEPATH""${LIBNAME}_" -SUFFIX="_visible.$FILETYPE" -DIRECTORY=$(cd `dirname $0` && pwd) +PREFIX="$BASEPATH" +SUFFIX=".$FILETYPE" +DIRECTORY="$(cd "$(dirname "$0")" && pwd)" -RESULTS=`gzip -cdf "$FASTQ_FILE" | $DIRECTORY/fastx_barcode_splitter.pl --bcfile "$BARCODE_FILE" --prefix "$PREFIX" --suffix "$SUFFIX" "$@"` +RESULTS=$(gzip -cdf "$FASTQ_FILE" | "$DIRECTORY/fastx_barcode_splitter.pl" --bcfile "$BARCODE_FILE" --prefix "$PREFIX" --suffix "$SUFFIX" "$@") if [ $? != 0 ]; then echo "error" fi # -# Convert the textual tab-separated table into simple HTML table, -# with the local path replaces with a valid URL -#HTMLSUMMARY=${PREFIX}stats_visible_html +# Convert the textual tab-separated table into simple HTML table echo "" -echo "$RESULTS" | sed "s|$BASEPATH\\(.*\\)|\\1|" | \ +echo "$RESULTS" | sed "s|$BASEPATH\\(.*\\)|\\1|" | \ perl -n -e '$_ =~ s|\t|\n"' echo "

" echo "

|g; print "
\n$_
" diff -r b7b3d008e2d3 -r 32304398ef67 test-data/.fastx_barcode_splitter1.out.swp Binary file test-data/.fastx_barcode_splitter1.out.swp has changed diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1.out --- a/test-data/fastx_barcode_splitter1.out Tue Oct 27 11:47:52 2015 -0400 +++ b/test-data/fastx_barcode_splitter1.out Fri Nov 13 12:43:14 2015 -0500 @@ -3,19 +3,19 @@ BarcodeCountLocation -BC111primary_3_BC1_visible.fastqsolexa +BC111BC1.fastqsolexa -BC212primary_3_BC2_visible.fastqsolexa +BC212BC2.fastqsolexa -BC39primary_3_BC3_visible.fastqsolexa +BC39BC3.fastqsolexa -BC41primary_3_BC4_visible.fastqsolexa +BC41BC4.fastqsolexa -unmatched9primary_3_unmatched_visible.fastqsolexa +unmatched9unmatched.fastqsolexa total42 diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1_BC1.out --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastx_barcode_splitter1_BC1.out Fri Nov 13 12:43:14 2015 -0500 @@ -0,0 +1,44 @@ +@CSHL_3_FC042AGLLWW:1:2:7:203 +GATCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GATCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GATCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GATCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GATCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTCTAGTAGTAGTAGA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTCTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTCTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1_BC2.out --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastx_barcode_splitter1_BC2.out Fri Nov 13 12:43:14 2015 -0500 @@ -0,0 +1,48 @@ +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCGTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCGTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCGTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCGTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTCGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTCGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTCTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +ATCTCGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1_BC3.out --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastx_barcode_splitter1_BC3.out Fri Nov 13 12:43:14 2015 -0500 @@ -0,0 +1,36 @@ +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGAATGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1_BC4.out --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastx_barcode_splitter1_BC4.out Fri Nov 13 12:43:14 2015 -0500 @@ -0,0 +1,4 @@ +@CSHL_3_FC042AGLLWW:1:2:7:203 +TGTCTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa \ No newline at end of file diff -r b7b3d008e2d3 -r 32304398ef67 test-data/fastx_barcode_splitter1_unmatched.out --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastx_barcode_splitter1_unmatched.out Fri Nov 13 12:43:14 2015 -0500 @@ -0,0 +1,36 @@ +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTACGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTACTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +GGTACGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTGAGTATACACAT ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa +@CSHL_3_FC042AGLLWW:1:2:7:203 +TAGTTTCTCTATGTACA ++CSHL_3_FC042AGLLWW:1:2:7:203 +aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 testoutput/fastx_barcode_splitter1.out --- a/testoutput/fastx_barcode_splitter1.out Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,24 +0,0 @@ - - - - - - - - -

-

-BarcodeCountLocation -
-BC111primary_3_BC1_visible_fastqsolexa -
-BC212primary_3_BC2_visible_fastqsolexa -
-BC39primary_3_BC3_visible_fastqsolexa -
-BC41primary_3_BC4_visible_fastqsolexa -
-unmatched9primary_3_unmatched_visible_fastqsolexa -
-total42 -
diff -r b7b3d008e2d3 -r 32304398ef67 tmp/BC1 --- a/tmp/BC1 Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,32 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/BC2 --- a/tmp/BC2 Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,32 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/BC3 diff -r b7b3d008e2d3 -r 32304398ef67 tmp/BC4 --- a/tmp/BC4 Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -TGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa \ No newline at end of file diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC1_visible.fastqsanger --- a/tmp/tmp_BC1_visible.fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,44 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC1_visible_fastqsanger --- a/tmp/tmp_BC1_visible_fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,44 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GATCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTAGTAGTAGTAGA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC2_visible.fastqsanger --- a/tmp/tmp_BC2_visible.fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,48 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC2_visible_fastqsanger --- a/tmp/tmp_BC2_visible_fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,48 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCGTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC3_visible.fastqsanger --- a/tmp/tmp_BC3_visible.fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,36 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC3_visible_fastqsanger --- a/tmp/tmp_BC3_visible_fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,36 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC4_visible.fastqsanger --- a/tmp/tmp_BC4_visible.fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -TGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa \ No newline at end of file diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_BC4_visible_fastqsanger --- a/tmp/tmp_BC4_visible_fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -TGTCTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa \ No newline at end of file diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_unmatched_visible.fastqsanger --- a/tmp/tmp_unmatched_visible.fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,36 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/tmp_unmatched_visible_fastqsanger --- a/tmp/tmp_unmatched_visible_fastqsanger Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,36 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp/unmatched --- a/tmp/unmatched Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,100 +0,0 @@ -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGTACGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -ATCTCGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -GGAATGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTGAGTATACACAT -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa -@CSHL_3_FC042AGLLWW:1:2:7:203 -TAGTTTCTCTATGTACA -+CSHL_3_FC042AGLLWW:1:2:7:203 -aab^V^aU]`aa^aZaa diff -r b7b3d008e2d3 -r 32304398ef67 tmp_stdio.txt --- a/tmp_stdio.txt Tue Oct 27 11:47:52 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,7 +0,0 @@ -Barcode Count Location -BC1 8 tmp/BC1 -BC2 8 tmp/BC2 -BC3 0 tmp/BC3 -BC4 1 tmp/BC4 -unmatched 25 tmp/unmatched -total 42