Mercurial > repos > lparsons > htseq_count
changeset 22:55ed198f2c1c draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/htseq_count commit 03f64004f90ac0a7be67ecfc355a7b361f3c3314
| author | iuc |
|---|---|
| date | Tue, 20 Sep 2016 17:51:06 -0400 |
| parents | a6dcb86af112 |
| children | 6e5c95760ab1 |
| files | README.rst htseq-count.xml tool_dependencies.xml |
| diffstat | 3 files changed, 34 insertions(+), 58 deletions(-) [+] |
line wrap: on
line diff
--- a/README.rst Tue Jul 28 13:29:18 2015 -0400 +++ b/README.rst Tue Sep 20 17:51:06 2016 -0400 @@ -12,15 +12,8 @@ Installation directly from the `Galaxy Toolshed <http://toolshed.g2.bx.psu.edu/view/lparsons/htseq_count>`_ is recommended. -Development ------------ - -Repository-Maintainer: Lance Parsons <lparsons@princeton.edu> - -Repository-Development: `https://bitbucket.org/lance_parsons/htseq_count_galaxy_wrapper <https://bitbucket.org/lance_parsons/htseq_count_galaxy_wrapper>`_ - -License -------- +Galaxy Wrapper License +---------------------- Copyright (c) 2012-2014, Lance R. Parsons <lparsons@princeton.edu> All rights reserved.
--- a/htseq-count.xml Tue Jul 28 13:29:18 2015 -0400 +++ b/htseq-count.xml Tue Sep 20 17:51:06 2016 -0400 @@ -1,10 +1,8 @@ -<tool id="htseq_count" name="htseq-count" version="0.6.1galaxy1"> +<tool id="htseq_count" name="htseq-count" version="0.6.1galaxy2"> <description> - Count aligned reads in a BAM file that overlap features in a GFF file</description> <requirements> - <requirement type="package" version="0.6.1">htseq</requirement> - <requirement type="package" version="1.7.1">numpy</requirement> + <requirement type="package" version="0.6.1.post1">htseq</requirement> <requirement type="package" version="0.1.19">samtools</requirement> - <requirement type="package" version="0.7.7">pysam</requirement> </requirements> <stdio> @@ -22,7 +20,7 @@ <command><![CDATA[ ##set up input files #set $reference_fasta_filename = "localref.fa" - #if $samout_conditional.samout: + #if $samout_conditional.samout == "Yes": #if str( $samout_conditional.reference_source.reference_source_selector ) == "history": ln -s "${samout_conditional.reference_source.ref_file}" "${reference_fasta_filename}" && samtools faidx "${reference_fasta_filename}" 2>&1 || echo "Error running samtools faidx for htseq-count" >&2 && @@ -30,11 +28,11 @@ #set $reference_fasta_filename = str( $samout_conditional.reference_source.ref_file.fields.path ) #end if #end if - #if $force_sort: + #if $force_sort == "True": #if $samfile.extension == 'bam': samtools sort -n "$samfile" "name_sorted_alignment" && #else - samtools view -Su -t ${reference_fasta_filename}.fai "$samfile" | samtools sort -n - "name_sorted_alignment" && + samtools view -Su -t "${reference_fasta_filename}.fai" "$samfile" | samtools sort -n - "name_sorted_alignment" && #end if #end if htseq-count @@ -43,10 +41,10 @@ --minaqual=$minaqual --type="$featuretype" --idattr="$idattr" - #if $samout_conditional.samout: + #if $samout_conditional.samout == "Yes": --samout=$__new_file_path__/${samoutfile.id}_tmp #end if - #if $force_sort: + #if $force_sort == "True": --order=name --format=bam name_sorted_alignment.bam @@ -57,8 +55,8 @@ #end if "$gfffile" | awk '{if ($1 ~ "no_feature|ambiguous|too_low_aQual|not_aligned|alignment_not_unique") print $0 | "cat 1>&2"; else print $0}' > $counts 2>$othercounts - #if $samout_conditional.samout: - && samtools view -Su -t ${reference_fasta_filename}.fai $__new_file_path__/${samoutfile.id}_tmp | samtools sort -o - sorted > $samoutfile + #if $samout_conditional.samout == "Yes": + && samtools view -Su -t "${reference_fasta_filename}.fai" "$__new_file_path__/${samoutfile.id}_tmp" | samtools sort -o - sorted > "$samoutfile" #end if ]]> </command> @@ -66,32 +64,34 @@ <inputs> <param format="sam,bam" name="samfile" type="data" label="Aligned SAM/BAM File"/> <param format="gff" name="gfffile" type="data" label="GFF File"/> - <param name="mode" type="select" label="Mode" help="(--mode)"> + <param name="mode" type="select" label="Mode" argument="(--mode)"> <help>Mode to handle reads overlapping more than one feature.</help> - <option value="union" selected="true">Union</option> + <option value="union" selected="True">Union</option> <option value="intersection-strict">Intersection (strict)</option> <option value="intersection-nonempty">Intersection (nonempty)</option> </param> - <param name="stranded" type="select" label="Stranded" help="(--stranded)"> + <param name="stranded" type="select" label="Stranded" argument="(--stranded)"> <help>Specify whether the data is from a strand-specific assay. 'Reverse' means yes with reversed strand interpretation.</help> - <option value="yes" selected="true">Yes</option> + <option value="yes" selected="True">Yes</option> <option value="no">No</option> <option value="reverse">Reverse</option> </param> - <param name="minaqual" type="integer" value="10" label="Minimum alignment quality"> - <help>Skip all reads with alignment quality lower than the given minimum value. (-minaqual)</help> + <param name="minaqual" type="integer" value="10" label="Minimum alignment quality" argument="--minaqual"> + <help>Skip all reads with alignment quality lower than the given minimum value.</help> </param> - <param name="featuretype" type="text" value="exon" label="Feature type"> - <help>Feature type (3rd column in GFF file) to be used. All features of other types are ignored. The default, suitable for RNA-Seq and Ensembl GTF files, is exon. (--type)</help> + <param name="featuretype" type="text" value="exon" label="Feature type" argument="--type"> + <help>Feature type (3rd column in GFF file) to be used. All features of other types are ignored. The default, suitable for RNA-Seq and Ensembl GTF files, is exon.</help> </param> - <param name="idattr" type="text" value="gene_id" label="ID Attribute"> + <param name="idattr" type="text" value="gene_id" label="ID Attribute" argument="--idattr"> <help>GFF attribute to be used as feature ID. Several GFF lines with the same feature ID will be considered as parts of the same feature. The feature ID is used to identity the counts in the output table. All features of the specified type MUST have a value for this attribute. The default, suitable for RNA-Seq and Ensembl GTF files, is gene_id.</help> </param> <conditional name="samout_conditional"> - <param name="samout" type="boolean" value="False" truevalue="True" falsevalue="False" label="Additional BAM Output"> + <param name="samout" type="select" label="Additional BAM Output"> <help>Write out all SAM alignment records into an output BAM file, annotating each line with its assignment to a feature or a special counter (as an optional field with tag ‘XF’).</help> + <option value="No" selected="True">Do not output additional BAM file</option> + <option value="Yes">Output additional BAM file</option> </param> - <when value="True"> + <when value="Yes"> <conditional name="reference_source"> <param name="reference_source_selector" type="select" label="Choose the source for the reference list"> <option value="cached">Locally cached</option> @@ -110,6 +110,9 @@ </when> </conditional> </when> + <when value="No"> + <!-- Do nothing --> + </when> </conditional> <param name="force_sort" type="boolean" value="False" truevalue="True" falsevalue="False" label="Force sorting of SAM/BAM file by NAME"> <help>This option can be used for for paired-end data that has many unmapped mates. Use this if you get the warning about paired end data missing or not being properly sorted.</help> @@ -120,7 +123,7 @@ <data format="tabular" name="counts" metadata_source="samfile" label="${tool.name} on ${on_string}"/> <data format="tabular" name="othercounts" metadata_source="samfile" label="${tool.name} on ${on_string} (no feature)"/> <data format="bam" name="samoutfile" metadata_source="samfile" label="${tool.name} on ${on_string} (BAM)"> - <filter>samout_conditional['samout']</filter> + <filter>samout_conditional['samout'] == "Yes"</filter> </data> </outputs> @@ -128,14 +131,14 @@ <test> <param name="samfile" value="htseq-test.sam" /> <param name="gfffile" value="htseq-test.gff" /> - <param name="samout" value="False" /> + <param name="samout" value="No" /> <output name="counts" file="htseq-test_counts.tsv" /> <output name="othercounts" file="htseq-test_othercounts.tsv" /> </test> <test> <param name="samfile" value="htseq-test.sam" /> <param name="gfffile" value="htseq-test.gff" /> - <param name="samout" value="False" /> + <param name="samout" value="No" /> <param name="force_sort" value="True" /> <output name="counts" file="htseq-test_counts.tsv" /> <output name="othercounts" file="htseq-test_othercounts.tsv" /> @@ -143,7 +146,7 @@ <test> <param name="samfile" value="htseq-test.bam" /> <param name="gfffile" value="htseq-test.gff" /> - <param name="samout" value="False" /> + <param name="samout" value="No" /> <output name="counts" file="htseq-test_counts.tsv" /> <output name="othercounts" file="htseq-test_othercounts.tsv" /> </test> @@ -151,7 +154,7 @@ <param name="samfile" value="htseq-test-paired.bam" /> <param name="singlepaired" value="paired" /> <param name="gfffile" value="htseq-test.gff" /> - <param name="samout" value="False" /> + <param name="samout" value="No" /> <output name="counts" file="htseq-test-paired_counts.tsv" /> <output name="othercounts" file="htseq-test-paired_othercounts.tsv" /> </test> @@ -159,7 +162,7 @@ <param name="samfile" value="htseq-test-paired.bam" /> <param name="singlepaired" value="paired" /> <param name="gfffile" value="htseq-test.gff" /> - <param name="samout" value="False" /> + <param name="samout" value="No" /> <param name="force_sort" value="True" /> <output name="counts" file="htseq-test-paired_counts.tsv" /> <output name="othercounts" file="htseq-test-paired_othercounts.tsv" /> @@ -294,7 +297,7 @@ year = {2015}, pmid = {25260700}, pages = {166--169}, - file = {Full Text PDF:/Users/lparsons/Library/Application Support/Firefox/Profiles/thd2t4je.default/zotero/storage/84XQB8V6/Anders et al. - 2015 - HTSeq—a Python framework to work with high-through.pdf:application/pdf;Snapshot:/Users/lparsons/Library/Application Support/Firefox/Profiles/thd2t4je.default/zotero/storage/JKUAUCKB/166.html:text/html} + } } </citation> </citations>
--- a/tool_dependencies.xml Tue Jul 28 13:29:18 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,20 +0,0 @@ -<?xml version="1.0"?> -<tool_dependency> - - <package name="samtools" version="0.1.19"> - <repository changeset_revision="95d2c4aefb5f" name="package_samtools_0_1_19" owner="devteam" toolshed="https://toolshed.g2.bx.psu.edu" /> - </package> - - <package name="pysam" version="0.7.7"> - <repository changeset_revision="0a5141bdf9d0" name="package_pysam_0_7_7" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> - </package> - - <package name="numpy" version="1.7.1"> - <repository changeset_revision="300877695495" name="package_numpy_1_7" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> - </package> - - <package name="htseq" version="0.6.1"> - <repository changeset_revision="fbb72996807d" name="package_htseq_0_6" owner="iuc" toolshed="https://toolshed.g2.bx.psu.edu" /> - </package> - -</tool_dependency>