diff cut_degen.xml @ 0:33b174991d3e draft default tip

planemo upload for repository https://github.com/mshortr/degenerateprimerremoval commit df5ba746a916e08e0847a727237f6bec5ef0511f-dirty
author megan-shortridge
date Tue, 15 Sep 2015 14:29:28 -0400
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/cut_degen.xml	Tue Sep 15 14:29:28 2015 -0400
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+<tool id="cut_degen" name="Cut degenerate primers from dataset" version="0.1.0">
+	<description> Degenerate primer removal tool for Fasta files</description>
+    <requirements>
+	<requirement type = "package" version = "1.9">numpy</requirement>
+	<requirement type = "package" version = "1.65">biopython</requirement>
+    </requirements>
+    <stdio>
+        <exit_code range="1:" />
+    </stdio>
+
+    <command interpreter="python">cut_degen.py $input1 $input2 $input3 $input4 $input5 $input6
+    </command>
+    <inputs>
+	<param name="input1" type="data" label="Input Fasta file" help="Please add a valid Fasta file!" format="fasta"/>
+	<param name="input2" type="text" label="Input adaptor" help="This will work also with degenerate adaptors. Type the adaptor here..." size="4x25" area="true"/>
+	<param name="input3" type="select" label="Keep reads without adaptor?" help="This is a True/False option, if True, reads that lack the adaptor will be kept. If false, they will be deleted from the dataset.">
+			<option value="True">True</option>
+			<option value="False">False</option>
+		</param>
+	<param name="input4" type="select" label = "Remove adaptors?" help="This is True/False option, if True, removes adaptors from sequences. If false, keeps the adaptors in the sequences.">
+			<option value="True">True</option>
+			<option value="False">False</option>
+		</param>
+	<param name="input5" type="select" multiple="False" label="5' or 3' end" help="Enter '5' to remove the primer from the 5' end, and enter '3' to remove the primer from the 3' end">
+        <option value="5">5</option>
+		<option value="3">3</option>
+        </param>
+        <param name="input6" type="text" label="Adaptor name" help="Enter an identifier for your adaptor to be recorded in the output .txt file" size="4x25" area="true">
+		</param>
+    </inputs>
+	<outputs>
+		<data name="output1" format="text" from_work_dir="output.txt" label="Degenerate adaptor removal on ${input1.name}(REPORT)"/>
+		<data name="output2" format="fasta" from_work_dir="output.fasta" label="Degenerate adaptor removal on ${input1.name}(READS)"/>
+    </outputs>
+	<tests>
+			<test>
+	<param name="input1" value="test.fasta"/>
+	<param name="input2"  value="GAACWAYWYCT"/>
+	<param name="input3" value="True"/>
+	<param name="input4" value="True"/>
+	<param name="input5" value="5"/>
+    <param name="input6"  value="test"/>
+	<output name="output1" file="output.txt"/>
+	<output name="output2" file="output.fasta"/>
+        </test>
+		<test>
+			<param name="input1" value="test3prime.fasta"/>
+			<param name="input2" value="GCTCGGTGGCGT"/>
+			<param name="input3" value="True"/>
+			<param name="input4" value="True"/>
+			<param name="input5" value="3"/>
+			<param name="input6" value="test2"/>
+			<output name="output1" file="output2.txt"/>
+			<output name="output2" file="output2.fasta"/>
+		</test>
+    </tests>
+    <help>
+This program takes Fasta files and removes a given degenerate primer sequence. There are a few arguments that you can put in:
+        1) You can check whether or not to keep reads that do not have the adaptor in it.
+        2) You can check whether or not to remove the adaptor sequences from your reads.
+        3) You can choose whether to cut adaptors from the 5', or 3' end of the molecules.
+		4) You can add an identifier for the adaptor which will show up in the output text file.
+    </help>
+	<citations>
+	<citation type="doi">10.1093/bioinformatics/btp163</citation>
+	</citations>
+</tool>