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author mheinzl
date Wed, 16 Oct 2019 10:07:17 -0400
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<?xml version="1.0" encoding="UTF-8"?>
<!-- galaxy version 16.04 -->
<tool id="fsd" name="FSD" version="1.0.9">
    <description>: Family Size Distribution of duplex sequencing tags</description>
        <requirement type="package" version="2.7">python</requirement>
        <requirement type="package" version="1.4.0">matplotlib</requirement>
        python2 '$__tool_directory__/' --inputFile1 '${file1}' --inputName1 '${}' 
--inputFile2 '${file2}' --inputName2 '${}' --inputFile3 '${file3}' --inputName3 '${}' 
--inputFile4 '${file4}' --inputName4 '${}' $log_axis $rel_freq --output_pdf $output_pdf --output_tabular $output_tabular 
        <param name="file1" type="data" format="tabular" label="Dataset 1: input tags" optional="false"/>
        <param name="file2" type="data" format="tabular" label="Dataset 2: input tags" optional="true"  />
        <param name="file3" type="data" format="tabular" label="Dataset 3: input tags" optional="true" />
        <param name="file4" type="data" format="tabular" label="Dataset 4: input tags" optional="true"  help="Input in tabular format with the family size, tags and the direction of the strand ('ab' or 'ba') for each family. Name of the files can have max. 34 charcters!"/>
        <param name="log_axis" type="boolean" label="log scale for y axis?" truevalue="" falsevalue="--log_axis" checked="False" help="Transform y axis in log scale."/>
        <param name="rel_freq" type="boolean" label="relative frequency?" truevalue="" falsevalue="--rel_freq" checked="False" help="If True, the relative frequencies instead of the absolute values are displayed in the plots."/>
        <data name="output_pdf" format="pdf" />
        <data name="output_tabular" format="tabular"/>
            <param name="file1" value=""/>
            <param name="file2" value=""/>
            <param name="file3" value=""/>
            <param name="file4" value=""/>
            <output name="output_pdf" file="fsd_output1.pdf" lines_diff="285"/>
            <output name="output_tabular" file=""/>
            <param name="file1" value=""/>
            <param name="file2" value=""/>
            <param name="file3" value=""/>
            <output name="output_pdf" file="fsd_output2.pdf" lines_diff="285"/>
            <output name="output_tabular" file=""/>

**What it does**
This tool will create a distribution of family sizes of each tag, which is separated after tags that have only the forward (ab) strand, the reverse (ba) strand or both strands (ab+ba) of the DCS and a family size distribution without separation is created. 
This tools expects a tabular file with the tags of all families, their sizes and information about forward (ab) and reverse (ba) strands:: 

The output is a PDF file with the plot and a tabular file with the data of the plot.
**About Author**
Author: Monika Heinzl
Department: Institute of Bioinformatics, Johannes Kepler University Linz, Austria
        <citation type="bibtex">
            author = {Heinzl, Monika},
            year = {2018},
            title = {Development of algorithms for the analysis of duplex sequencing data}