changeset 17:2e517a54eedc draft

planemo upload for repository https://github.com/monikaheinzl/duplexanalysis_galaxy/tree/master/tools/fsd commit b8a2f7b7615b2bcd3b602027af31f4e677da94f6-dirty
author mheinzl
date Tue, 02 Apr 2019 05:10:09 -0400
parents 6bd9ef49d013
children c825a29a7d9f
files fsd.py fsd.xml test-data/Test_data.tabular test-data/Test_data2.tabular test-data/Test_data3.tabular test-data/Test_data4.tabular test-data/fsd_data1.tab test-data/fsd_data2.tab test-data/fsd_data3.tab test-data/fsd_data4.tab test-data/fsd_output1.pdf test-data/fsd_output1.tab test-data/fsd_output2.pdf test-data/fsd_output2.tab test-data/output_file.pdf test-data/output_file.tabular test-data/output_file2.pdf test-data/output_file2.tabular
diffstat 18 files changed, 1046 insertions(+), 830 deletions(-) [+]
line wrap: on
line diff
--- a/fsd.py	Mon Oct 08 05:50:18 2018 -0400
+++ b/fsd.py	Tue Apr 02 05:10:09 2019 -0400
@@ -45,19 +45,17 @@
 
 
 def compare_read_families(argv):
+
     parser = make_argparser()
     args = parser.parse_args(argv[1:])
-
     firstFile = args.inputFile1
     name1 = args.inputName1
-
     secondFile = args.inputFile2
     name2 = args.inputName2
     thirdFile = args.inputFile3
     name3 = args.inputName3
     fourthFile = args.inputFile4
     name4 = args.inputName4
-
     title_file = args.output_tabular
     title_file2 = args.output_pdf
 
@@ -90,24 +88,32 @@
             data_array_list.append(file1)
 
             legend = "\n\n\n{}".format(name1)
-            plt.text(0.1, 0.11, legend, size=12, transform=plt.gcf().transFigure)
-            legend1 = "singletons:\nabsolute nr.\n{:,}".format(numpy.bincount(data1)[1])
-            plt.text(0.4, 0.11, legend1, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.05, 0.11, legend, size=10, transform=plt.gcf().transFigure)
+            legend1 = "singletons:\nnr. of tags\n{:,}".format(numpy.bincount(data1)[1])
+            plt.text(0.32, 0.11, legend1, size=10, transform=plt.gcf().transFigure)
 
-            legend3 = "rel. freq\n{:.3f}".format(float(numpy.bincount(data1)[1]) / len(data1))
-            plt.text(0.5, 0.11, legend3, size=12, transform=plt.gcf().transFigure)
+            legend3 = "freq. of tags\n{:.3f}".format(float(numpy.bincount(data1)[1]) / len(data1))
+            plt.text(0.41, 0.11, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3b = "PE reads\n{:.3f}".format(float(numpy.bincount(data1)[1]) / sum(integers))
+            plt.text(0.5, 0.11, legend3b, size=10, transform=plt.gcf().transFigure)
 
-            legend4 = "family size > 20:\nabsolute nr.\n{:,}".format(numpy.bincount(data1)[len(numpy.bincount(data1)) - 1].astype(int))
-            plt.text(0.6, 0.11, legend4, size=12, transform=plt.gcf().transFigure)
+            legend4 = "family size > 20:\nnr. of tags\n{:,} ({:.3f})".format(numpy.bincount(data1)[len(numpy.bincount(data1)) - 1].astype(int), float(numpy.bincount(data1)[len(numpy.bincount(data1)) - 1]) / len(data1))
+            plt.text(0.58, 0.11, legend4, size=10, transform=plt.gcf().transFigure)
+
+            legend5 = "PE reads\n{:,} ({:.3f})".format(sum(integers[integers > 20]), float(sum(integers[integers > 20])) / sum(integers))
+            plt.text(0.70, 0.11, legend5, size=10, transform=plt.gcf().transFigure)
 
-            legend5 = "rel. freq\n{:.3f}".format(float(numpy.bincount(data1)[len(numpy.bincount(data1)) - 1]) / len(data1))
-            plt.text(0.7, 0.11, legend5, size=12, transform=plt.gcf().transFigure)
+            legend6 = "total nr. of\ntags\n{:,}".format(len(data1))
+            plt.text(0.82, 0.11, legend6, size=10, transform=plt.gcf().transFigure)
 
-            legend6 = "total length\n{:,}".format(len(data1))
-            plt.text(0.8, 0.11, legend6, size=12, transform=plt.gcf().transFigure)
+            legend6b = "PE reads\n{:,}".format(sum(integers))
+            plt.text(0.89, 0.11, legend6b, size=10, transform=plt.gcf().transFigure)
 
         if secondFile != str(None):
             file2 = readFileReferenceFree(secondFile)
+            integers2 = numpy.array(file2[:, 0]).astype(int)  # keep original family sizes
+
             data2 = numpy.asarray(file2[:, 0]).astype(int)
             bigFamilies2 = numpy.where(data2 > 20)[0]
             data2[bigFamilies2] = 22
@@ -117,25 +123,34 @@
             label.append(name2)
             data_array_list.append(file2)
 
-            plt.text(0.1, 0.09, name2, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.05, 0.09, name2, size=10, transform=plt.gcf().transFigure)
 
             legend1 = "{:,}".format(numpy.bincount(data2)[1])
-            plt.text(0.4, 0.09, legend1, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.32, 0.09, legend1, size=10, transform=plt.gcf().transFigure)
 
             legend3 = "{:.3f}".format(float(numpy.bincount(data2)[1]) / len(data2))
-            plt.text(0.5, 0.09, legend3, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.41, 0.09, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3b = "{:.3f}".format(float(numpy.bincount(data2)[1]) / sum(integers2))
+            plt.text(0.5, 0.09, legend3b, size=10, transform=plt.gcf().transFigure)
 
-            legend4 = "{:,}".format(numpy.bincount(data2)[len(numpy.bincount(data2)) - 1].astype(int))
-            plt.text(0.6, 0.09, legend4, size=12, transform=plt.gcf().transFigure)
+            legend4 = "{:,} ({:.3f})".format(
+                numpy.bincount(data2)[len(numpy.bincount(data2)) - 1].astype(int),
+                float(numpy.bincount(data2)[len(numpy.bincount(data2)) - 1]) / len(data2))
+            plt.text(0.58, 0.09, legend4, size=10, transform=plt.gcf().transFigure)
 
-            legend5 = "{:.3f}".format(float(numpy.bincount(data2)[len(numpy.bincount(data2)) - 1]) / len(data2))
-            plt.text(0.7, 0.09, legend5, size=12, transform=plt.gcf().transFigure)
+            legend5 = "{:,} ({:.3f})".format(sum(integers2[integers2 > 20]), float(sum(integers2[integers2 > 20])) / sum(integers2))
+            plt.text(0.70, 0.09, legend5, size=10, transform=plt.gcf().transFigure)
 
             legend6 = "{:,}".format(len(data2))
-            plt.text(0.8, 0.09, legend6, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.82, 0.09, legend6, size=10, transform=plt.gcf().transFigure)
+
+            legend6b = "{:,}".format(sum(integers2))
+            plt.text(0.89, 0.09, legend6b, size=10, transform=plt.gcf().transFigure)
 
         if thirdFile != str(None):
             file3 = readFileReferenceFree(thirdFile)
+            integers3 = numpy.array(file3[:, 0]).astype(int)  # keep original family sizes
 
             data3 = numpy.asarray(file3[:, 0]).astype(int)
             bigFamilies3 = numpy.where(data3 > 20)[0]
@@ -146,25 +161,35 @@
             label.append(name3)
             data_array_list.append(file3)
 
-            plt.text(0.1, 0.07, name3, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.05, 0.07, name3, size=10, transform=plt.gcf().transFigure)
 
             legend1 = "{:,}".format(numpy.bincount(data3)[1])
-            plt.text(0.4, 0.07, legend1, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.32, 0.07, legend1, size=10, transform=plt.gcf().transFigure)
 
             legend3 = "{:.3f}".format(float(numpy.bincount(data3)[1]) / len(data3))
-            plt.text(0.5, 0.07, legend3, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.41, 0.07, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3b = "{:.3f}".format(float(numpy.bincount(data3)[1]) / sum(integers3))
+            plt.text(0.5, 0.07, legend3b, size=10, transform=plt.gcf().transFigure)
 
-            legend4 = "{:,}".format(numpy.bincount(data3)[len(numpy.bincount(data3)) - 1].astype(int))
-            plt.text(0.6, 0.07, legend4, size=12, transform=plt.gcf().transFigure)
+            legend4 = "{:,} ({:.3f})".format(
+                numpy.bincount(data3)[len(numpy.bincount(data3)) - 1].astype(int),
+                float(numpy.bincount(data3)[len(numpy.bincount(data3)) - 1]) / len(data3))
+            plt.text(0.58, 0.07, legend4, size=10, transform=plt.gcf().transFigure)
 
-            legend5 = "{:.3f}".format(float(numpy.bincount(data3)[len(numpy.bincount(data3)) - 1]) / len(data3))
-            plt.text(0.7, 0.07, legend5, size=12, transform=plt.gcf().transFigure)
+            legend5 = "{:,} ({:.3f})".format(sum(integers3[integers3 > 20]),
+                                             float(sum(integers3[integers3 > 20])) / sum(integers3))
+            plt.text(0.70, 0.07, legend5, size=10, transform=plt.gcf().transFigure)
 
             legend6 = "{:,}".format(len(data3))
-            plt.text(0.8, 0.07, legend6, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.82, 0.07, legend6, size=10, transform=plt.gcf().transFigure)
+
+            legend6b = "{:,}".format(sum(integers3))
+            plt.text(0.89, 0.07, legend6b, size=10, transform=plt.gcf().transFigure)
 
         if fourthFile != str(None):
             file4 = readFileReferenceFree(fourthFile)
+            integers4 = numpy.array(file4[:, 0]).astype(int)  # keep original family sizes
 
             data4 = numpy.asarray(file4[:, 0]).astype(int)
 
@@ -176,28 +201,37 @@
             label.append(name4)
             data_array_list.append(file4)
 
-            plt.text(0.1, 0.05, name4, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.05, 0.05, name4, size=10, transform=plt.gcf().transFigure)
 
             legend1 = "{:,}".format(numpy.bincount(data4)[1])
-            plt.text(0.4, 0.05, legend1, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.32, 0.05, legend1, size=10, transform=plt.gcf().transFigure)
 
-            legend4 = "{:.3f}".format(float(numpy.bincount(data4)[1]) / len(data4))
-            plt.text(0.5, 0.05, legend4, size=12, transform=plt.gcf().transFigure)
+            legend3 = "{:.3f}".format(float(numpy.bincount(data4)[1]) / len(data4))
+            plt.text(0.41, 0.05, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3b = "{:.3f}".format(float(numpy.bincount(data4)[1]) / sum(integers4))
+            plt.text(0.5, 0.05, legend3b, size=10, transform=plt.gcf().transFigure)
 
-            legend4 = "{:,}".format(numpy.bincount(data4)[len(numpy.bincount(data4)) - 1].astype(int))
-            plt.text(0.6, 0.05, legend4, size=12, transform=plt.gcf().transFigure)
+            legend4 = "{:,} ({:.3f})".format(
+                numpy.bincount(data4)[len(numpy.bincount(data4)) - 1].astype(int),
+                float(numpy.bincount(data4)[len(numpy.bincount(data4)) - 1]) / len(data4))
+            plt.text(0.58, 0.05, legend4, size=10, transform=plt.gcf().transFigure)
 
-            legend5 = "{:.3f}".format(float(numpy.bincount(data4)[len(numpy.bincount(data4)) - 1]) / len(data4))
-            plt.text(0.7, 0.05, legend5, size=12, transform=plt.gcf().transFigure)
+            legend5 = "{:,} ({:.3f})".format(sum(integers4[integers4 > 20]),
+                                             float(sum(integers4[integers4 > 20])) / sum(integers4))
+            plt.text(0.70, 0.05, legend5, size=10, transform=plt.gcf().transFigure)
 
             legend6 = "{:,}".format(len(data4))
-            plt.text(0.8, 0.05, legend6, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.82, 0.05, legend6, size=10, transform=plt.gcf().transFigure)
+
+            legend6b = "{:,}".format(sum(integers4))
+            plt.text(0.89, 0.05, legend6b, size=10, transform=plt.gcf().transFigure)
 
         maximumX = numpy.amax(numpy.concatenate(list_to_plot))
         minimumX = numpy.amin(numpy.concatenate(list_to_plot))
 
         counts = plt.hist(list_to_plot, bins=range(minimumX, maximumX + 1), stacked=False, edgecolor="black",
-                          linewidth=1, label=label, align="left", alpha=0.7, rwidth=0.8)
+                          linewidth=1, label=label, align="left", rwidth=0.8, alpha=0.7)
 
         ticks = numpy.arange(minimumX - 1, maximumX, 1)
         ticks1 = map(str, ticks)
@@ -242,53 +276,71 @@
                 output_file.write("{}{}".format(int(sum(i)), sep))
 
         # Family size distribution after DCS and SSCS
-        for dataset, data, name_file in zip(list_to_plot, data_array_list, label):
+        for dataset, data_o, name_file in zip(list_to_plot, data_array_list, label):
             maximumX = numpy.amax(dataset)
             minimumX = numpy.amin(dataset)
 
-            tags = numpy.array(data[:, 2])
-            seq = numpy.array(data[:, 1])
+            tags = numpy.array(data_o[:, 2])
+            seq = numpy.array(data_o[:, 1])
             data = numpy.array(dataset)
-
+            data_o = numpy.array(data_o[:, 0]).astype(int)
             # find all unique tags and get the indices for ALL tags, but only once
             u, index_unique, c = numpy.unique(numpy.array(seq), return_counts=True, return_index=True)
             d = u[c > 1]
 
             # get family sizes, tag for duplicates
             duplTags_double = data[numpy.in1d(seq, d)]
-            duplTags = duplTags_double[0::2]  # ab of DCS
-            duplTagsBA = duplTags_double[1::2]  # ba of DCS
+            duplTags_double_o = data_o[numpy.in1d(seq, d)]
 
-            # duplTags_double_tag = tags[numpy.in1d(seq, d)]
-            # duplTags_double_seq = seq[numpy.in1d(seq, d)]
+            duplTags = duplTags_double[0::2]  # ab of DCS
+            duplTags_o = duplTags_double_o[0::2]  # ab of DCS
+
+            duplTagsBA = duplTags_double[1::2]  # ba of DCS
+            duplTagsBA_o = duplTags_double_o[1::2]  # ba of DCS
 
             # get family sizes for SSCS with no partner
             ab = numpy.where(tags == "ab")[0]
             abSeq = seq[ab]
+            ab_o = data_o[ab]
             ab = data[ab]
+
             ba = numpy.where(tags == "ba")[0]
             baSeq = seq[ba]
+            ba_o = data_o[ba]
             ba = data[ba]
 
             dataAB = ab[numpy.in1d(abSeq, d, invert=True)]
+            dataAB_o = ab_o[numpy.in1d(abSeq, d, invert=True)]
+
             dataBA = ba[numpy.in1d(baSeq, d, invert=True)]
+            dataBA_o = ba_o[numpy.in1d(baSeq, d, invert=True)]
 
             list1 = [duplTags_double, dataAB, dataBA]  # list for plotting
 
             # information for family size >= 3
             dataAB_FS3 = dataAB[dataAB >= 3]
+            dataAB_FS3_o = dataAB_o[dataAB_o >= 3]
             dataBA_FS3 = dataBA[dataBA >= 3]
+            dataBA_FS3_o = dataBA_o[dataBA_o >= 3]
             ab_FS3 = ab[ab >= 3]
             ba_FS3 = ba[ba >= 3]
+            ab_FS3_o = ab_o[ab_o >= 3]
+            ba_FS3_o = ba_o[ba_o >= 3]
 
             duplTags_FS3 = duplTags[(duplTags >= 3) & (duplTagsBA >= 3)]  # ab+ba with FS>=3
             duplTags_FS3_BA = duplTagsBA[(duplTags >= 3) & (duplTagsBA >= 3)]  # ba+ab with FS>=3
             duplTags_double_FS3 = len(duplTags_FS3) + len(duplTags_FS3_BA)  # both ab and ba strands with FS>=3
 
-            fig = plt.figure()
+            # original FS
+            duplTags_FS3_o = duplTags_o[(duplTags_o >= 3) & (duplTagsBA_o >= 3)]  # ab+ba with FS>=3
+            duplTags_FS3_BA_o = duplTagsBA_o[(duplTags_o >= 3) & (duplTagsBA_o >= 3)]  # ba+ab with FS>=3
+            duplTags_double_FS3_o = sum(duplTags_FS3_o) + sum(duplTags_FS3_BA_o)  # both ab and ba strands with FS>=3
 
+            fig = plt.figure()
             plt.subplots_adjust(bottom=0.3)
-            counts = plt.hist(list1, bins=range(minimumX, maximumX + 1), stacked=True, label=["duplex", "ab", "ba"], edgecolor="black", linewidth=1, align="left", color=["#FF0000", "#5FB404", "#FFBF00"])
+            counts = plt.hist(list1, bins=range(minimumX, maximumX + 1), stacked=True, label=["duplex", "ab", "ba"],
+                              edgecolor="black", linewidth=1, align="left", color=["#FF0000", "#5FB404", "#FFBF00"],
+                              rwidth=0.8)
             # tick labels of x axis
             ticks = numpy.arange(minimumX - 1, maximumX, 1)
             ticks1 = map(str, ticks)
@@ -298,33 +350,56 @@
             last = counts[0][2][len(counts[0][0]) - 1]  # large families
 
             plt.legend(loc='upper right', fontsize=14, bbox_to_anchor=(0.9, 1), frameon=True)
-            # plt.title(name1, fontsize=14)
+            plt.title(name_file, fontsize=14)
             plt.xlabel("Family size", fontsize=14)
             plt.ylabel("Absolute Frequency", fontsize=14)
             plt.margins(0.01, None)
             plt.grid(b=True, which="major", color="#424242", linestyle=":")
 
             # extra information beneath the plot
-            legend = "SSCS ab= \nSSCS ba= \nDCS (total)= \nlength of dataset="
-            plt.text(0.1, 0.09, legend, size=12, transform=plt.gcf().transFigure)
+            legend = "SSCS ab= \nSSCS ba= \nDCS (total)= \ntotal nr. of tags="
+            plt.text(0.1, 0.09, legend, size=10, transform=plt.gcf().transFigure)
+
+            legend = "nr. of tags\n\n{:,}\n{:,}\n{:,} ({:,})\n{:,}".format(len(dataAB), len(dataBA), len(duplTags), len(duplTags_double), (len(dataAB) + len(dataBA) + len(duplTags)))
+            plt.text(0.23, 0.09, legend, size=10, transform=plt.gcf().transFigure)
 
-            legend = "absolute numbers\n\n{:,}\n{:,}\n{:,} ({:,})\n{:,}".format(len(dataAB), len(dataBA), len(duplTags), len(duplTags_double), (len(dataAB) + len(dataBA) + len(duplTags)))
-            plt.text(0.35, 0.09, legend, size=12, transform=plt.gcf().transFigure)
+            legend5 = "PE reads\n\n{:,}\n{:,}\n{:,} ({:,})\n{:,}".format(sum(dataAB_o), sum(dataBA_o), sum(duplTags_o), sum(duplTags_double_o), (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)))
+            plt.text(0.38, 0.09, legend5, size=10, transform=plt.gcf().transFigure)
 
-            legend = "relative frequencies\nunique\n{:.3f}\n{:.3f}\n{:.3f}\n{:,}".format(float(len(dataAB)) / (len(dataAB) + len(dataBA) + len(duplTags)), float(len(dataBA)) / (len(dataAB) + len(dataBA) + len(duplTags)), float(len(duplTags)) / (len(dataAB) + len(dataBA) + len(duplTags)), (len(dataAB) + len(dataBA) + len(duplTags)))
-            plt.text(0.54, 0.09, legend, size=12, transform=plt.gcf().transFigure)
+            legend = "rel. freq. of tags\nunique\n{:.3f}\n{:.3f}\n{:.3f}\n{:,}".format(float(len(dataAB)) / (len(dataAB) + len(dataBA) + len(duplTags)), float(len(dataBA)) / (len(dataAB) + len(dataBA) + len(duplTags)), float(len(duplTags)) / (len(dataAB) + len(dataBA) + len(duplTags)), (len(dataAB) + len(dataBA) + len(duplTags)))
+            plt.text(0.54, 0.09, legend, size=10, transform=plt.gcf().transFigure)
 
             legend = "total\n{:.3f}\n{:.3f}\n{:.3f} ({:.3f})\n{:,}".format(float(len(dataAB)) / (len(ab) + len(ba)), float(len(dataBA)) / (len(ab) + len(ba)), float(len(duplTags)) / (len(ab) + len(ba)), float(len(duplTags_double)) / (len(ab) + len(ba)), (len(ab) + len(ba)))
-            plt.text(0.64, 0.09, legend, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.64, 0.09, legend, size=10, transform=plt.gcf().transFigure)
 
             legend1 = "\nsingletons:\nfamily size > 20:"
-            plt.text(0.1, 0.03, legend1, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.1, 0.03, legend1, size=10, transform=plt.gcf().transFigure)
 
             legend4 = "{:,}\n{:,}".format(singl.astype(int), last.astype(int))
-            plt.text(0.35, 0.03, legend4, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.23, 0.03, legend4, size=10, transform=plt.gcf().transFigure)
 
             legend3 = "{:.3f}\n{:.3f}".format(singl / len(data), last / len(data))
-            plt.text(0.54, 0.03, legend3, size=12, transform=plt.gcf().transFigure)
+            plt.text(0.64, 0.03, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3 = "\n\n{:,}".format(sum(data_o[data_o > 20]))
+            plt.text(0.38, 0.03, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend3 = "{:.3f}\n{:.3f}".format(float(singl)/sum(data_o), float(sum(data_o[data_o > 20])) / sum(data_o))
+            plt.text(0.84, 0.03, legend3, size=10, transform=plt.gcf().transFigure)
+
+            legend = "PE reads\nunique\n{:.3f}\n{:.3f}\n{:.3f}\n{:,}".format(
+                float(sum(dataAB_o)) / (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)),
+                float(sum(dataBA_o)) / (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)),
+                float(sum(duplTags_o)) / (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)),
+                (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)))
+            plt.text(0.74, 0.09, legend, size=10, transform=plt.gcf().transFigure)
+
+            legend = "total\n{:.3f}\n{:.3f}\n{:.3f} ({:.3f})\n{:,}".format(
+                float(sum(dataAB_o)) / (sum(ab_o) + sum(ba_o)),
+                float(sum(dataBA_o)) / (sum(ab_o) + sum(ba_o)),
+                float(sum(duplTags_o)) / (sum(ab_o) + sum(ba_o)),
+                float(sum(duplTags_double_o)) / (sum(ab_o) + sum(ba_o)), (sum(ab_o) + sum(ba_o)))
+            plt.text(0.84, 0.09, legend, size=10, transform=plt.gcf().transFigure)
 
             pdf.savefig(fig)
             plt.close()
@@ -336,23 +411,62 @@
             output_file.write("absolute frequency:{}{}\n".format(sep, count[len(count) - 1]))
             output_file.write("relative frequency:{}{:.3f}\n\n".format(sep, float(count[len(count) - 1]) / sum(count)))
 
-            output_file.write("{}singletons:{}{}family size > 20:\n".format(sep, sep, sep))
-            output_file.write("{}absolute nr.{}rel. freq{}absolute nr.{}rel. freq{}total length\n".format(sep, sep, sep, sep, sep))
-            output_file.write("{}{}{}{}{:.3f}{}{}{}{:.3f}{}{}\n\n".format(name_file, sep, singl.astype(int), sep, singl / len(data), sep, last.astype(int), sep, last / len(data), sep, len(data)))
+            output_file.write("{}singletons:{}{}{}family size > 20:\n".format(sep, sep, sep, sep))
+            output_file.write("{}nr. of tags{}rel. freq of tags{}rel.freq of PE reads{}nr. of tags{}rel. freq of tags{}nr. of PE reads{}rel. freq of PE reads{}total nr. of tags{}total nr. of PE reads\n".format(sep, sep, sep, sep, sep, sep, sep, sep, sep))
+            output_file.write("{}{}{}{}{:.3f}{}{:.3f}{}{}{}{:.3f}{}{}{}{:.3f}{}{}{}{}\n\n".format(
+                name_file, sep, singl.astype(int), sep, singl / len(data), sep, float(singl)/sum(data_o), sep,
+                last.astype(int), sep, last / len(data), sep, sum(data_o[data_o > 20]), sep, float(sum(data_o[data_o > 20])) / sum(data_o), sep, len(data), sep, sum(data_o)))
 
             # information for FS >= 1
-            output_file.write("The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)\nWhereas the total frequencies were calculated from the whole dataset (=including the DCS).\n\n")
-            output_file.write("FS >= 1{}{}unique:{}total:\n".format(sep, sep, sep))
-            output_file.write("nr./rel. freq of ab={}{}{}{:.3f}{}{:.3f}\n".format(sep, len(dataAB), sep, float(len(dataAB)) / (len(dataAB) + len(dataBA) + len( duplTags)), sep, float(len(dataAB)) / (len(ab) + len(ba))))
-            output_file.write("nr./rel. freq of ba={}{}{}{:.3f}{}{:.3f}\n".format(sep, len(dataBA), sep, float(len(dataBA)) / (len(dataBA) + len(dataBA) + len(duplTags)), sep, float(len(dataBA)) / (len(ba) + len(ba))))
-            output_file.write("nr./rel. freq of DCS (total)={}{} ({}){}{:.3f}{}{:.3f} ({:.3f})\n".format(sep, len(duplTags), len(duplTags_double), sep, float(len(duplTags)) / (len(dataAB) + len(dataBA) + len(duplTags)), sep, float(len(duplTags)) / ( len(ab) + len(ba)), float(len(duplTags_double)) / (len(ab) + len(ba))))
-            output_file.write("length of dataset={}{}{}{}{}{}\n".format(sep, (len(dataAB) + len(dataBA) + len(duplTags)), sep, (len(dataAB) + len(dataBA) + len(duplTags)), sep, (len(ab) + len(ba))))
+            output_file.write("The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)\n"
+                              "Whereas the total frequencies were calculated from the whole dataset (=including the DCS).\n\n")
+            output_file.write("FS >= 1{}nr. of tags{}nr. of PE reads{}rel. freq of tags{}{}rel. freq of PE reads:\n".format(sep, sep, sep, sep, sep))
+            output_file.write("{}{}{}unique:{}total{}unique{}total:\n".format(sep, sep, sep, sep, sep, sep))
+            output_file.write("SSCS ab{}{}{}{}{}{:.3f}{}{:.3f}{}{:.3f}{}{:.3f}\n".format(
+                sep, len(dataAB), sep, sum(dataAB_o), sep, float(len(dataAB)) / (len(dataAB) + len(dataBA) + len(duplTags)),
+                sep, float(sum(dataAB_o)) / (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)), sep,
+                float(len(dataAB)) / (len(ab) + len(ba)), sep, float(sum(dataAB_o)) / (sum(ab_o) + sum(ba_o))))
+            output_file.write("SSCS ba{}{}{}{}{}{:.3f}{}{:.3f}{}{:.3f}{}{:.3f}\n".format(
+                sep, len(dataBA), sep, sum(dataBA_o), sep, float(len(dataBA)) / (len(dataBA) + len(dataBA) + len(duplTags)),
+                sep, float(sum(dataBA_o)) / (sum(dataBA_o) + sum(dataBA_o) + sum(duplTags_o)), sep, float(len(dataBA)) / (len(ba) + len(ba)),
+                sep, float(sum(dataBA_o)) / (sum(ba_o) + sum(ba_o))))
+            output_file.write("DCS (total){}{} ({}){}{} ({}){}{:.3f}{}{:.3f} ({:.3f}){}{:.3f}{}{:.3f} ({:.3f})\n".format(
+                sep, len(duplTags), len(duplTags_double), sep, sum(duplTags_o), sum(duplTags_double_o), sep,
+                float(len(duplTags)) / (len(dataAB) + len(dataBA) + len(duplTags)), sep,
+                float(len(duplTags)) / (len(ab) + len(ba)), float(len(duplTags_double)) / (len(ab) + len(ba)), sep,
+                float(sum(duplTags_o)) / (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)), sep,
+                float(sum(duplTags_o)) / (sum(ab_o) + sum(ba_o)), float(sum(duplTags_double_o)) / (sum(ab_o) + sum(ba_o))))
+            output_file.write("total nr. of tags{}{}{}{}{}{}{}{}{}{}{}{}\n".format(
+                sep, (len(dataAB) + len(dataBA) + len(duplTags)), sep, (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)), sep,
+                (len(dataAB) + len(dataBA) + len(duplTags)), sep, (len(ab) + len(ba)), sep,
+                (sum(dataAB_o) + sum(dataBA_o) + sum(duplTags_o)), sep, (sum(ab_o) + sum(ba_o))))
             # information for FS >= 3
-            output_file.write("FS >= 3{}{}unique:{}total:\n".format(sep, sep, sep))
-            output_file.write("nr./rel. freq of ab={}{}{}{:.3f}{}{:.3f}\n".format(sep, len(dataAB_FS3), sep, float(len(dataAB_FS3)) / (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, float(len(dataAB_FS3)) / (len(ab_FS3) + len(ba_FS3))))
-            output_file.write("nr./rel. freq of ba={}{}{}{:.3f}{}{:.3f}\n".format(sep, len(dataBA_FS3), sep, float(len(dataBA_FS3)) / (len(dataBA_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, float(len(dataBA_FS3)) / (len(ba_FS3) + len(ba_FS3))))
-            output_file.write("nr./rel. freq of DCS (total)={}{} ({}){}{:.3f}{}{:.3f} ({:.3f})\n".format(sep, len(duplTags_FS3), duplTags_double_FS3, sep, float(len( duplTags_FS3)) / (len(dataBA_FS3) + len(duplTags_FS3)), sep, float(len(duplTags_FS3)) / (len(ab_FS3) + len(ba_FS3)), float(duplTags_double_FS3) / (len(ab_FS3) + len(ba_FS3))))
-            output_file.write("length of dataset={}{}{}{}{}{}\n".format(sep, (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, (len(ab_FS3) + len(ba_FS3))))
+            output_file.write("\nFS >= 3{}nr. of tags{}nr. of PE reads{}rel. freq of tags{}{}rel. freq of PE reads:\n".format(sep, sep, sep, sep, sep))
+            output_file.write("{}{}{}unique:{}total{}unique{}total:\n".format(sep, sep, sep, sep, sep, sep))
+            output_file.write("SSCS ab{}{}{}{}{}{:.3f}{}{:.3f}{}{:.3f}{}{:.3f}\n".format(
+                sep, len(dataAB_FS3), sep, sum(dataAB_FS3_o), sep,
+                float(len(dataAB_FS3)) / (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep,
+                float(len(dataAB_FS3)) / (len(dataBA_FS3) + len(dataBA_FS3) + duplTags_double_FS3),
+                sep, float(sum(dataAB_FS3_o)) / (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + sum(duplTags_FS3_o)),
+                sep, float(sum(dataAB_FS3_o)) / (sum(dataBA_FS3_o) + sum(dataBA_FS3_o) + duplTags_double_FS3_o)))
+            output_file.write("SSCS ba{}{}{}{}{}{:.3f}{}{:.3f}{}{:.3f}{}{:.3f}\n".format(
+                sep, len(dataBA_FS3), sep, sum(dataBA_FS3_o), sep,
+                float(len(dataBA_FS3)) / (len(dataBA_FS3) + len(dataBA_FS3) + len(duplTags_FS3)),
+                sep, float(len(dataBA_FS3)) / (len(dataBA_FS3) + len(dataBA_FS3) + duplTags_double_FS3),
+                sep, float(sum(dataBA_FS3_o)) / (sum(dataBA_FS3_o) + sum(dataBA_FS3_o) + sum(duplTags_FS3_o)),
+                sep, float(sum(dataBA_FS3_o)) / (sum(dataBA_FS3_o) + sum(dataBA_FS3_o) + duplTags_double_FS3_o)))
+            output_file.write("DCS (total){}{} ({}){}{} ({}){}{:.3f}{}{:.3f} ({:.3f}){}{:.3f}{}{:.3f} ({:.3f})\n".format(
+                sep, len(duplTags_FS3), duplTags_double_FS3,  sep, sum(duplTags_FS3_o), duplTags_double_FS3_o, sep,
+                float(len(duplTags_FS3)) / (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep,
+                float(len(duplTags_FS3)) / (len(dataAB_FS3) + len(dataBA_FS3) + duplTags_double_FS3),
+                float(duplTags_double_FS3) / (len(dataAB_FS3) + len(dataBA_FS3) + duplTags_double_FS3),
+                sep, float(sum(duplTags_FS3_o)) / (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + sum(duplTags_FS3_o)), sep,
+                float(sum(duplTags_FS3_o)) / (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + duplTags_double_FS3_o),
+                float(duplTags_double_FS3_o) / (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + duplTags_double_FS3_o)))
+            output_file.write("total nr. of tags{}{}{}{}{}{}{}{}{}{}{}{}\n".format(
+                sep, (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + sum(duplTags_FS3_o)),
+                sep, (len(dataAB_FS3) + len(dataBA_FS3) + len(duplTags_FS3)), sep, (len(dataAB_FS3) + len(dataBA_FS3) + duplTags_double_FS3),
+                sep, (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + sum(duplTags_FS3_o)), sep, (sum(dataAB_FS3_o) + sum(dataBA_FS3_o) + duplTags_double_FS3_o)))
 
             output_file.write("\nValues from family size distribution\n")
             output_file.write("{}duplex{}ab{}ba{}sum\n".format(sep, sep, sep, sep))
--- a/fsd.xml	Mon Oct 08 05:50:18 2018 -0400
+++ b/fsd.xml	Tue Apr 02 05:10:09 2019 -0400
@@ -24,19 +24,19 @@
     </outputs>
     <tests>
         <test>
-            <param name="file1" value="Test_data.tabular"/>
-            <param name="file2" value="Test_data2.tabular"/>
-            <param name="file3" value="Test_data3.tabular"/>
-            <param name="file4" value="Test_data4.tabular"/>
-            <output name="output_pdf" file="output_file.pdf" lines_diff="285"/>
-            <output name="output_tabular" file="output_file.tabular"/>
+            <param name="file1" value="fsd_data1.tab"/>
+            <param name="file2" value="fsd_data2.tab"/>
+            <param name="file3" value="fsd_data3.tab"/>
+            <param name="file4" value="fsd_data4.tab"/>
+            <output name="output_pdf" file="fsd_output1.pdf" lines_diff="285"/>
+            <output name="output_tabular" file="fsd_output1.tab"/>
         </test>
         <test>
-            <param name="file1" value="Test_data.tabular"/>
-            <param name="file2" value="Test_data2.tabular"/>
-            <param name="file3" value="Test_data3.tabular"/>
-            <output name="output_pdf" file="output_file2.pdf" lines_diff="285"/>
-            <output name="output_tabular" file="output_file2.tabular"/>
+            <param name="file1" value="fsd_data1.tab"/>
+            <param name="file2" value="fsd_data2.tab"/>
+            <param name="file3" value="fsd_data3.tab"/>
+            <output name="output_pdf" file="fsd_output2.pdf" lines_diff="285"/>
+            <output name="output_tabular" file="fsd_output2.tab"/>
         </test>
     </tests>
     <help><![CDATA[
--- a/test-data/Test_data.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,112 +0,0 @@
-1	AAAAAAAAAAAAAACCAAAACTTC	ba
-1	AAAAAAAAAAAAACCAGGCGTCGA	ba
-1	AAAAAAAAAAAAAGCTCCACGTTG	ba
-1	AAAAAAAAAAAAATCGTGGTTTGT	ba
-1	AAAAAAAAAAAAATTCACCCTTGT	ba
-7	AAAAAAAAAAAACACACTTAACTT	ba
-1	AAAAAAAAAAAACAGTGTTGAGAC	ba
-4	AAAAAAAAAAAACCGCTCCTCACA	ba
-1	AAAAAAAAAAAAGGCAACACAGAA	ab
-2	AAAAAAAAAAAATCTTTCTTTGAG	ab
-1	AAAAAAAAAAAATTGGGTTCCTTA	ab
-1	AAAAAAAAAAAGAGTCGCACCCAG	ba
-4	AAAAAAAAAAAGATCGTGGTTTGT	ba
-1	AAAAAAAAAAAGCGCAACACAGAA	ab
-3	AAAAAAAAAAAGGGCAACACAGAA	ab
-1	AAAAAAAAAAAGTAGCCCTAAACG	ab
-1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
-1	AAAAAAAAAAATATCATAGACTCT	ab
-6	AAAAAAAAAAATATTCACCCTTGT	ba
-1	AAAAAAAAAAATATTCGAAAGTTA	ba
-3	AAAAAAAAAAATCACACTTAACTT	ba
-1	AAAAAAAAAAATCCGCTCCTCACA	ba
-1	AAAAAAAAAAATTAACTAAACTTA	ab
-1	AAAAAAAAAACAAATTCTATTATT	ab
-1	AAAAAAAAAACTCCCAGATTTTTT	ab
-1	AAAAAAAAAACTTCTGCTTGGCGG	ba
-11	AAAAAAAAAAGAATCGTGGTTTGT	ba
-5	AAAAAAAAAAGATAGCCCTAAACG	ab
-1	AAAAAAAAAAGCAATAATGCCAGT	ab
-2	AAAAAAAAAAGTACCGCACTCTCA	ba
-1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
-1	AAAAAAAAAATAACTTCAATAATG	ba
-2	AAAAAAAAAATAATCATAGACTCT	ab
-1	AAAAAAAAAATAGTCTCACATTTA	ab
-1	AAAAAAAAAATATAACCTTTGGCG	ab
-3	AAAAAAAAACAAAATTCTATTATT	ab
-1	AAAAAAAAACAAGTACGCGGCATT	ab
-1	AAAAAAAAACAAGTACGCGGTATT	ab
-1	AAAAAAAAACAATATCGAATTAAC	ab
-3	AAAAAAAAACACGGTGAGACAAGG	ba
-1	AAAAAAAAACACGTTTCTCCCCTT	ba
-1	AAAAAAAAACATATCGTCCCGAGC	ba
-1	AAAAAAAAACCTACCTGAGGCCCC	ab
-3	AAAAAAAAACCTTATTACAGCGGA	ab
-1	AAAAAAAAACGATTCTCTGTATCT	ba
-1	AAAAAAAAACGTACCGCACTCTCA	ba
-4	AAAAAAAAACTACCCAGATTTTTT	ba
-1	AAAAAAAAACTAGATGAGACGACC	ba
-4	AAAAAAAAACTGTCTGCTTGGCGG	ba
-1	AAAAAAAAAGAAGTTTAATTTTAA	ab
-1	AAAAAAAAAGAATGCCTAAGACGA	ba
-6	AAAAAAAAAGACCGGCCTTAGACA	ba
-1	AAAAAAAAAGATATCGTGGTTTGT	ba
-1	AAAAAAAAAGCAATACTCAAGCTG	ba
-6	AAAAAAAAAGCAATGTCTAAGCCT	ba
-1	AAAAAAAAAGCACTGTCTAAGCCT	ab
-2	AAAAAAAAAGCTAATAATGCCAGT	ab
-1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
-1	AAAAAAAAATAAAGGTCCGAATCT	ab
-1	AAAAAAAAATAAATGAGAGTGTAA	ba
-8	AAAAAAAAATAAGTCTCACATTTA	ab
-1	AAAAAAAAATAATAACCTCTGGCG	ab
-10	AAAAAAAAATAATAACCTTTGGCG	ab
-1	AAAAAAAAATAATCCCCTTTGTCG	ab
-6	AAAAAAAAATACGCAAACGCTGAG	ab
-4	AAAAAAAAATAGATCATAGACTCT	ab
-10	AAAAAAAAATAGATCATAGACTCT	ba
-10	AAAAAAAAATAGTAGGATTTCATG	ba
-7	AAAAAAAAATATGAATACCCTCGT	ba
-1	AAAAAAAAATATGCCACTTGATCC	ba
-1	AAAAAAAAATATTCTGCCACTTGA	ba
-3	AAAAAAAAATCAAACCAAGAGGAC	ba
-1	AAAAAAAAATCAGTACCCCTAAAC	ab
-12	AAAAAAAAATCCTAGTTAATGAAG	ba
-1	AAAAAAAAATCGATTCTTTATGCG	ab
-1	AAAAAAAAATGTCTGAAAATATCT	ab
-4	AAAAAAAAATGTCTGAAAATATCT	ba
-1	AAAAAAAAATTTCCGCAGACCGTT	ba
-8	AAAAAAAAATTTGGGCTACTACAA	ba
-1	AAAAAAAACAAAATTAGAACCCTT	ab
-1	AAAAAAAACAAACCGCTCCTCACA	ba
-5	AAAAAAAACAACGTACGCGGTATT	ab
-4	AAAAAAAACAATATCGTTGATATG	ba
-4	AAAAAAAACAATCACGTTAATAGG	ab
-1	AAAAAAAACAGAATCGTGGTTTGT	ba
-1	AAAAAAAACCAAATCGTTGATATG	ba
-9	AAAAAAAACCAAGTCCAGGCATCT	ba
-2	AAAAAAAACCACGGTGAGACAAGG	ba
-1	AAAAAAAACCGCCCAACTGCCGGT	ab
-5	AAAAAAAACCTCTCAACCCCAAAT	ba
-7	AAAAAAAACCTCTTGCGATGTTGT	ab
-1	AAAAAAAACCTCTTGCGCTGTTGT	ab
-1	AAAAAAAACCTCTTGTGATGTTGT	ab
-12	AAAAAAAACCTGAGCAATGGTTCC	ab
-3	AAAAAAAACCTTGACCCTCACATG	ba
-6	AAAAAAAACCTTGCACTCGTCCTA	ba
-9	AAAAAAAACGAAATAAAAAAACCT	ba
-1	AAAAAAAACGACCGGCCTTAGACA	ba
-4	AAAAAAAACGCCACCACCCCCTTT	ab
-12	AAAAAAAACGCCACGGGCACTATT	ba
-13	AAAAAAAACGTATCAGTAGATCCT	ab
-1	AAAAAAAACTAGTAGGATTTCATG	ba
-3	AAAAAAAACTATAGAAAATCCATT	ba
-1	AAAAAAAACTATTCTATTTCCGAT	ba
-13	AAAAAAAACTGATCTGCTTGGCGG	ba
-8	AAAAAAAACTTGCGAATAGCATCG	ba
-4	AAAAAAAACTTGTTATCAAAACGT	ab
-1	AAAAAAAAGAAAAGTTCAACACGC	ba
-1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
-13	AAAAAAAAGAGAGTTTAGTCATGG	ab
-1	AAAAAAAAGAGAGTTTAGTCATGG	ba
-1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- a/test-data/Test_data2.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,112 +0,0 @@
-1	AAAAAAAAAAAAAACCAAAACTTC	ba
-1	AAAAAAAAAAAAACCAGGCGTCGA	ba
-1	AAAAAAAAAAAAAGCTCCACGTTG	ba
-1	AAAAAAAAAAAAATCGTGGTTTGT	ba
-1	AAAAAAAAAAAAATTCACCCTTGT	ba
-7	AAAAAAAAAAAACACACTTAACTT	ba
-1	AAAAAAAAAAAACAGTGTTGAGAC	ba
-4	AAAAAAAAAAAACCGCTCCTCACA	ba
-1	AAAAAAAAAAAAGGCAACACAGAA	ab
-2	AAAAAAAAAAAATCTTTCTTTGAG	ab
-1	AAAAAAAAAAAATTGGGTTCCTTA	ab
-1	AAAAAAAAAAAGAGTCGCACCCAG	ba
-4	AAAAAAAAAAAGATCGTGGTTTGT	ba
-1	AAAAAAAAAAAGCGCAACACAGAA	ab
-3	AAAAAAAAAAAGGGCAACACAGAA	ab
-1	AAAAAAAAAAAGTAGCCCTAAACG	ab
-1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
-1	AAAAAAAAAAATATCATAGACTCT	ab
-6	AAAAAAAAAAATATTCACCCTTGT	ba
-1	AAAAAAAAAAATATTCGAAAGTTA	ba
-3	AAAAAAAAAAATCACACTTAACTT	ba
-1	AAAAAAAAAAATCCGCTCCTCACA	ba
-1	AAAAAAAAAAATTAACTAAACTTA	ab
-1	AAAAAAAAAACAAATTCTATTATT	ab
-1	AAAAAAAAAACTCCCAGATTTTTT	ab
-1	AAAAAAAAAACTTCTGCTTGGCGG	ba
-11	AAAAAAAAAAGAATCGTGGTTTGT	ba
-5	AAAAAAAAAAGATAGCCCTAAACG	ab
-1	AAAAAAAAAAGCAATAATGCCAGT	ab
-2	AAAAAAAAAAGTACCGCACTCTCA	ba
-1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
-1	AAAAAAAAAATAACTTCAATAATG	ba
-2	AAAAAAAAAATAATCATAGACTCT	ab
-1	AAAAAAAAAATAGTCTCACATTTA	ab
-1	AAAAAAAAAATATAACCTTTGGCG	ab
-3	AAAAAAAAACAAAATTCTATTATT	ab
-1	AAAAAAAAACAAGTACGCGGCATT	ab
-1	AAAAAAAAACAAGTACGCGGTATT	ab
-1	AAAAAAAAACAATATCGAATTAAC	ab
-3	AAAAAAAAACACGGTGAGACAAGG	ba
-1	AAAAAAAAACACGTTTCTCCCCTT	ba
-1	AAAAAAAAACATATCGTCCCGAGC	ba
-1	AAAAAAAAACCTACCTGAGGCCCC	ab
-3	AAAAAAAAACCTTATTACAGCGGA	ab
-1	AAAAAAAAACGATTCTCTGTATCT	ba
-1	AAAAAAAAACGTACCGCACTCTCA	ba
-4	AAAAAAAAACTACCCAGATTTTTT	ba
-1	AAAAAAAAACTAGATGAGACGACC	ba
-4	AAAAAAAAACTGTCTGCTTGGCGG	ba
-1	AAAAAAAAAGAAGTTTAATTTTAA	ab
-1	AAAAAAAAAGAATGCCTAAGACGA	ba
-6	AAAAAAAAAGACCGGCCTTAGACA	ba
-1	AAAAAAAAAGATATCGTGGTTTGT	ba
-1	AAAAAAAAAGCAATACTCAAGCTG	ba
-6	AAAAAAAAAGCAATGTCTAAGCCT	ba
-1	AAAAAAAAAGCACTGTCTAAGCCT	ab
-2	AAAAAAAAAGCTAATAATGCCAGT	ab
-1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
-1	AAAAAAAAATAAAGGTCCGAATCT	ab
-1	AAAAAAAAATAAATGAGAGTGTAA	ba
-8	AAAAAAAAATAAGTCTCACATTTA	ab
-1	AAAAAAAAATAATAACCTCTGGCG	ab
-10	AAAAAAAAATAATAACCTTTGGCG	ab
-1	AAAAAAAAATAATCCCCTTTGTCG	ab
-6	AAAAAAAAATACGCAAACGCTGAG	ab
-4	AAAAAAAAATAGATCATAGACTCT	ab
-10	AAAAAAAAATAGATCATAGACTCT	ba
-10	AAAAAAAAATAGTAGGATTTCATG	ba
-7	AAAAAAAAATATGAATACCCTCGT	ba
-1	AAAAAAAAATATGCCACTTGATCC	ba
-1	AAAAAAAAATATTCTGCCACTTGA	ba
-3	AAAAAAAAATCAAACCAAGAGGAC	ba
-1	AAAAAAAAATCAGTACCCCTAAAC	ab
-12	AAAAAAAAATCCTAGTTAATGAAG	ba
-1	AAAAAAAAATCGATTCTTTATGCG	ab
-1	AAAAAAAAATGTCTGAAAATATCT	ab
-4	AAAAAAAAATGTCTGAAAATATCT	ba
-1	AAAAAAAAATTTCCGCAGACCGTT	ba
-8	AAAAAAAAATTTGGGCTACTACAA	ba
-1	AAAAAAAACAAAATTAGAACCCTT	ab
-1	AAAAAAAACAAACCGCTCCTCACA	ba
-5	AAAAAAAACAACGTACGCGGTATT	ab
-4	AAAAAAAACAATATCGTTGATATG	ba
-4	AAAAAAAACAATCACGTTAATAGG	ab
-1	AAAAAAAACAGAATCGTGGTTTGT	ba
-1	AAAAAAAACCAAATCGTTGATATG	ba
-9	AAAAAAAACCAAGTCCAGGCATCT	ba
-2	AAAAAAAACCACGGTGAGACAAGG	ba
-1	AAAAAAAACCGCCCAACTGCCGGT	ab
-5	AAAAAAAACCTCTCAACCCCAAAT	ba
-7	AAAAAAAACCTCTTGCGATGTTGT	ab
-1	AAAAAAAACCTCTTGCGCTGTTGT	ab
-1	AAAAAAAACCTCTTGTGATGTTGT	ab
-12	AAAAAAAACCTGAGCAATGGTTCC	ab
-3	AAAAAAAACCTTGACCCTCACATG	ba
-6	AAAAAAAACCTTGCACTCGTCCTA	ba
-9	AAAAAAAACGAAATAAAAAAACCT	ba
-1	AAAAAAAACGACCGGCCTTAGACA	ba
-4	AAAAAAAACGCCACCACCCCCTTT	ab
-12	AAAAAAAACGCCACGGGCACTATT	ba
-13	AAAAAAAACGTATCAGTAGATCCT	ab
-1	AAAAAAAACTAGTAGGATTTCATG	ba
-3	AAAAAAAACTATAGAAAATCCATT	ba
-1	AAAAAAAACTATTCTATTTCCGAT	ba
-13	AAAAAAAACTGATCTGCTTGGCGG	ba
-8	AAAAAAAACTTGCGAATAGCATCG	ba
-4	AAAAAAAACTTGTTATCAAAACGT	ab
-1	AAAAAAAAGAAAAGTTCAACACGC	ba
-1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
-13	AAAAAAAAGAGAGTTTAGTCATGG	ab
-1	AAAAAAAAGAGAGTTTAGTCATGG	ba
-1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- a/test-data/Test_data3.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,112 +0,0 @@
-1	AAAAAAAAAAAAAACCAAAACTTC	ba
-1	AAAAAAAAAAAAACCAGGCGTCGA	ba
-1	AAAAAAAAAAAAAGCTCCACGTTG	ba
-1	AAAAAAAAAAAAATCGTGGTTTGT	ba
-1	AAAAAAAAAAAAATTCACCCTTGT	ba
-7	AAAAAAAAAAAACACACTTAACTT	ba
-1	AAAAAAAAAAAACAGTGTTGAGAC	ba
-4	AAAAAAAAAAAACCGCTCCTCACA	ba
-1	AAAAAAAAAAAAGGCAACACAGAA	ab
-2	AAAAAAAAAAAATCTTTCTTTGAG	ab
-1	AAAAAAAAAAAATTGGGTTCCTTA	ab
-1	AAAAAAAAAAAGAGTCGCACCCAG	ba
-4	AAAAAAAAAAAGATCGTGGTTTGT	ba
-1	AAAAAAAAAAAGCGCAACACAGAA	ab
-3	AAAAAAAAAAAGGGCAACACAGAA	ab
-1	AAAAAAAAAAAGTAGCCCTAAACG	ab
-1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
-1	AAAAAAAAAAATATCATAGACTCT	ab
-6	AAAAAAAAAAATATTCACCCTTGT	ba
-1	AAAAAAAAAAATATTCGAAAGTTA	ba
-3	AAAAAAAAAAATCACACTTAACTT	ba
-1	AAAAAAAAAAATCCGCTCCTCACA	ba
-1	AAAAAAAAAAATTAACTAAACTTA	ab
-1	AAAAAAAAAACAAATTCTATTATT	ab
-1	AAAAAAAAAACTCCCAGATTTTTT	ab
-1	AAAAAAAAAACTTCTGCTTGGCGG	ba
-11	AAAAAAAAAAGAATCGTGGTTTGT	ba
-5	AAAAAAAAAAGATAGCCCTAAACG	ab
-1	AAAAAAAAAAGCAATAATGCCAGT	ab
-2	AAAAAAAAAAGTACCGCACTCTCA	ba
-1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
-1	AAAAAAAAAATAACTTCAATAATG	ba
-2	AAAAAAAAAATAATCATAGACTCT	ab
-1	AAAAAAAAAATAGTCTCACATTTA	ab
-1	AAAAAAAAAATATAACCTTTGGCG	ab
-3	AAAAAAAAACAAAATTCTATTATT	ab
-1	AAAAAAAAACAAGTACGCGGCATT	ab
-1	AAAAAAAAACAAGTACGCGGTATT	ab
-1	AAAAAAAAACAATATCGAATTAAC	ab
-3	AAAAAAAAACACGGTGAGACAAGG	ba
-1	AAAAAAAAACACGTTTCTCCCCTT	ba
-1	AAAAAAAAACATATCGTCCCGAGC	ba
-1	AAAAAAAAACCTACCTGAGGCCCC	ab
-3	AAAAAAAAACCTTATTACAGCGGA	ab
-1	AAAAAAAAACGATTCTCTGTATCT	ba
-1	AAAAAAAAACGTACCGCACTCTCA	ba
-4	AAAAAAAAACTACCCAGATTTTTT	ba
-1	AAAAAAAAACTAGATGAGACGACC	ba
-4	AAAAAAAAACTGTCTGCTTGGCGG	ba
-1	AAAAAAAAAGAAGTTTAATTTTAA	ab
-1	AAAAAAAAAGAATGCCTAAGACGA	ba
-6	AAAAAAAAAGACCGGCCTTAGACA	ba
-1	AAAAAAAAAGATATCGTGGTTTGT	ba
-1	AAAAAAAAAGCAATACTCAAGCTG	ba
-6	AAAAAAAAAGCAATGTCTAAGCCT	ba
-1	AAAAAAAAAGCACTGTCTAAGCCT	ab
-2	AAAAAAAAAGCTAATAATGCCAGT	ab
-1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
-1	AAAAAAAAATAAAGGTCCGAATCT	ab
-1	AAAAAAAAATAAATGAGAGTGTAA	ba
-8	AAAAAAAAATAAGTCTCACATTTA	ab
-1	AAAAAAAAATAATAACCTCTGGCG	ab
-10	AAAAAAAAATAATAACCTTTGGCG	ab
-1	AAAAAAAAATAATCCCCTTTGTCG	ab
-6	AAAAAAAAATACGCAAACGCTGAG	ab
-4	AAAAAAAAATAGATCATAGACTCT	ab
-10	AAAAAAAAATAGATCATAGACTCT	ba
-10	AAAAAAAAATAGTAGGATTTCATG	ba
-7	AAAAAAAAATATGAATACCCTCGT	ba
-1	AAAAAAAAATATGCCACTTGATCC	ba
-1	AAAAAAAAATATTCTGCCACTTGA	ba
-3	AAAAAAAAATCAAACCAAGAGGAC	ba
-1	AAAAAAAAATCAGTACCCCTAAAC	ab
-12	AAAAAAAAATCCTAGTTAATGAAG	ba
-1	AAAAAAAAATCGATTCTTTATGCG	ab
-1	AAAAAAAAATGTCTGAAAATATCT	ab
-4	AAAAAAAAATGTCTGAAAATATCT	ba
-1	AAAAAAAAATTTCCGCAGACCGTT	ba
-8	AAAAAAAAATTTGGGCTACTACAA	ba
-1	AAAAAAAACAAAATTAGAACCCTT	ab
-1	AAAAAAAACAAACCGCTCCTCACA	ba
-5	AAAAAAAACAACGTACGCGGTATT	ab
-4	AAAAAAAACAATATCGTTGATATG	ba
-4	AAAAAAAACAATCACGTTAATAGG	ab
-1	AAAAAAAACAGAATCGTGGTTTGT	ba
-1	AAAAAAAACCAAATCGTTGATATG	ba
-9	AAAAAAAACCAAGTCCAGGCATCT	ba
-2	AAAAAAAACCACGGTGAGACAAGG	ba
-1	AAAAAAAACCGCCCAACTGCCGGT	ab
-5	AAAAAAAACCTCTCAACCCCAAAT	ba
-7	AAAAAAAACCTCTTGCGATGTTGT	ab
-1	AAAAAAAACCTCTTGCGCTGTTGT	ab
-1	AAAAAAAACCTCTTGTGATGTTGT	ab
-12	AAAAAAAACCTGAGCAATGGTTCC	ab
-3	AAAAAAAACCTTGACCCTCACATG	ba
-6	AAAAAAAACCTTGCACTCGTCCTA	ba
-9	AAAAAAAACGAAATAAAAAAACCT	ba
-1	AAAAAAAACGACCGGCCTTAGACA	ba
-4	AAAAAAAACGCCACCACCCCCTTT	ab
-12	AAAAAAAACGCCACGGGCACTATT	ba
-13	AAAAAAAACGTATCAGTAGATCCT	ab
-1	AAAAAAAACTAGTAGGATTTCATG	ba
-3	AAAAAAAACTATAGAAAATCCATT	ba
-1	AAAAAAAACTATTCTATTTCCGAT	ba
-13	AAAAAAAACTGATCTGCTTGGCGG	ba
-8	AAAAAAAACTTGCGAATAGCATCG	ba
-4	AAAAAAAACTTGTTATCAAAACGT	ab
-1	AAAAAAAAGAAAAGTTCAACACGC	ba
-1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
-13	AAAAAAAAGAGAGTTTAGTCATGG	ab
-1	AAAAAAAAGAGAGTTTAGTCATGG	ba
-1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- a/test-data/Test_data4.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,112 +0,0 @@
-1	AAAAAAAAAAAAAACCAAAACTTC	ba
-1	AAAAAAAAAAAAACCAGGCGTCGA	ba
-1	AAAAAAAAAAAAAGCTCCACGTTG	ba
-1	AAAAAAAAAAAAATCGTGGTTTGT	ba
-1	AAAAAAAAAAAAATTCACCCTTGT	ba
-7	AAAAAAAAAAAACACACTTAACTT	ba
-1	AAAAAAAAAAAACAGTGTTGAGAC	ba
-4	AAAAAAAAAAAACCGCTCCTCACA	ba
-1	AAAAAAAAAAAAGGCAACACAGAA	ab
-2	AAAAAAAAAAAATCTTTCTTTGAG	ab
-1	AAAAAAAAAAAATTGGGTTCCTTA	ab
-1	AAAAAAAAAAAGAGTCGCACCCAG	ba
-4	AAAAAAAAAAAGATCGTGGTTTGT	ba
-1	AAAAAAAAAAAGCGCAACACAGAA	ab
-3	AAAAAAAAAAAGGGCAACACAGAA	ab
-1	AAAAAAAAAAAGTAGCCCTAAACG	ab
-1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
-1	AAAAAAAAAAATATCATAGACTCT	ab
-6	AAAAAAAAAAATATTCACCCTTGT	ba
-1	AAAAAAAAAAATATTCGAAAGTTA	ba
-3	AAAAAAAAAAATCACACTTAACTT	ba
-1	AAAAAAAAAAATCCGCTCCTCACA	ba
-1	AAAAAAAAAAATTAACTAAACTTA	ab
-1	AAAAAAAAAACAAATTCTATTATT	ab
-1	AAAAAAAAAACTCCCAGATTTTTT	ab
-1	AAAAAAAAAACTTCTGCTTGGCGG	ba
-11	AAAAAAAAAAGAATCGTGGTTTGT	ba
-5	AAAAAAAAAAGATAGCCCTAAACG	ab
-1	AAAAAAAAAAGCAATAATGCCAGT	ab
-2	AAAAAAAAAAGTACCGCACTCTCA	ba
-1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
-1	AAAAAAAAAATAACTTCAATAATG	ba
-2	AAAAAAAAAATAATCATAGACTCT	ab
-1	AAAAAAAAAATAGTCTCACATTTA	ab
-1	AAAAAAAAAATATAACCTTTGGCG	ab
-3	AAAAAAAAACAAAATTCTATTATT	ab
-1	AAAAAAAAACAAGTACGCGGCATT	ab
-1	AAAAAAAAACAAGTACGCGGTATT	ab
-1	AAAAAAAAACAATATCGAATTAAC	ab
-3	AAAAAAAAACACGGTGAGACAAGG	ba
-1	AAAAAAAAACACGTTTCTCCCCTT	ba
-1	AAAAAAAAACATATCGTCCCGAGC	ba
-1	AAAAAAAAACCTACCTGAGGCCCC	ab
-3	AAAAAAAAACCTTATTACAGCGGA	ab
-1	AAAAAAAAACGATTCTCTGTATCT	ba
-1	AAAAAAAAACGTACCGCACTCTCA	ba
-4	AAAAAAAAACTACCCAGATTTTTT	ba
-1	AAAAAAAAACTAGATGAGACGACC	ba
-4	AAAAAAAAACTGTCTGCTTGGCGG	ba
-1	AAAAAAAAAGAAGTTTAATTTTAA	ab
-1	AAAAAAAAAGAATGCCTAAGACGA	ba
-6	AAAAAAAAAGACCGGCCTTAGACA	ba
-1	AAAAAAAAAGATATCGTGGTTTGT	ba
-1	AAAAAAAAAGCAATACTCAAGCTG	ba
-6	AAAAAAAAAGCAATGTCTAAGCCT	ba
-1	AAAAAAAAAGCACTGTCTAAGCCT	ab
-2	AAAAAAAAAGCTAATAATGCCAGT	ab
-1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
-1	AAAAAAAAATAAAGGTCCGAATCT	ab
-1	AAAAAAAAATAAATGAGAGTGTAA	ba
-8	AAAAAAAAATAAGTCTCACATTTA	ab
-1	AAAAAAAAATAATAACCTCTGGCG	ab
-10	AAAAAAAAATAATAACCTTTGGCG	ab
-1	AAAAAAAAATAATCCCCTTTGTCG	ab
-6	AAAAAAAAATACGCAAACGCTGAG	ab
-4	AAAAAAAAATAGATCATAGACTCT	ab
-10	AAAAAAAAATAGATCATAGACTCT	ba
-10	AAAAAAAAATAGTAGGATTTCATG	ba
-7	AAAAAAAAATATGAATACCCTCGT	ba
-1	AAAAAAAAATATGCCACTTGATCC	ba
-1	AAAAAAAAATATTCTGCCACTTGA	ba
-3	AAAAAAAAATCAAACCAAGAGGAC	ba
-1	AAAAAAAAATCAGTACCCCTAAAC	ab
-12	AAAAAAAAATCCTAGTTAATGAAG	ba
-1	AAAAAAAAATCGATTCTTTATGCG	ab
-1	AAAAAAAAATGTCTGAAAATATCT	ab
-4	AAAAAAAAATGTCTGAAAATATCT	ba
-1	AAAAAAAAATTTCCGCAGACCGTT	ba
-8	AAAAAAAAATTTGGGCTACTACAA	ba
-1	AAAAAAAACAAAATTAGAACCCTT	ab
-1	AAAAAAAACAAACCGCTCCTCACA	ba
-5	AAAAAAAACAACGTACGCGGTATT	ab
-4	AAAAAAAACAATATCGTTGATATG	ba
-4	AAAAAAAACAATCACGTTAATAGG	ab
-1	AAAAAAAACAGAATCGTGGTTTGT	ba
-1	AAAAAAAACCAAATCGTTGATATG	ba
-9	AAAAAAAACCAAGTCCAGGCATCT	ba
-2	AAAAAAAACCACGGTGAGACAAGG	ba
-1	AAAAAAAACCGCCCAACTGCCGGT	ab
-5	AAAAAAAACCTCTCAACCCCAAAT	ba
-7	AAAAAAAACCTCTTGCGATGTTGT	ab
-1	AAAAAAAACCTCTTGCGCTGTTGT	ab
-1	AAAAAAAACCTCTTGTGATGTTGT	ab
-12	AAAAAAAACCTGAGCAATGGTTCC	ab
-3	AAAAAAAACCTTGACCCTCACATG	ba
-6	AAAAAAAACCTTGCACTCGTCCTA	ba
-9	AAAAAAAACGAAATAAAAAAACCT	ba
-1	AAAAAAAACGACCGGCCTTAGACA	ba
-4	AAAAAAAACGCCACCACCCCCTTT	ab
-12	AAAAAAAACGCCACGGGCACTATT	ba
-13	AAAAAAAACGTATCAGTAGATCCT	ab
-1	AAAAAAAACTAGTAGGATTTCATG	ba
-3	AAAAAAAACTATAGAAAATCCATT	ba
-1	AAAAAAAACTATTCTATTTCCGAT	ba
-13	AAAAAAAACTGATCTGCTTGGCGG	ba
-8	AAAAAAAACTTGCGAATAGCATCG	ba
-4	AAAAAAAACTTGTTATCAAAACGT	ab
-1	AAAAAAAAGAAAAGTTCAACACGC	ba
-1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
-13	AAAAAAAAGAGAGTTTAGTCATGG	ab
-1	AAAAAAAAGAGAGTTTAGTCATGG	ba
-1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_data1.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,112 @@
+1	AAAAAAAAAAAAAACCAAAACTTC	ba
+1	AAAAAAAAAAAAACCAGGCGTCGA	ba
+1	AAAAAAAAAAAAAGCTCCACGTTG	ba
+1	AAAAAAAAAAAAATCGTGGTTTGT	ba
+1	AAAAAAAAAAAAATTCACCCTTGT	ba
+7	AAAAAAAAAAAACACACTTAACTT	ba
+1	AAAAAAAAAAAACAGTGTTGAGAC	ba
+4	AAAAAAAAAAAACCGCTCCTCACA	ba
+1	AAAAAAAAAAAAGGCAACACAGAA	ab
+2	AAAAAAAAAAAATCTTTCTTTGAG	ab
+1	AAAAAAAAAAAATTGGGTTCCTTA	ab
+1	AAAAAAAAAAAGAGTCGCACCCAG	ba
+21	AAAAAAAAAAAGATCGTGGTTTGT	ba
+1	AAAAAAAAAAAGCGCAACACAGAA	ab
+3	AAAAAAAAAAAGGGCAACACAGAA	ab
+1	AAAAAAAAAAAGTAGCCCTAAACG	ab
+1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
+1	AAAAAAAAAAATATCATAGACTCT	ab
+6	AAAAAAAAAAATATTCACCCTTGT	ba
+1	AAAAAAAAAAATATTCGAAAGTTA	ba
+3	AAAAAAAAAAATCACACTTAACTT	ba
+1	AAAAAAAAAAATCCGCTCCTCACA	ba
+1	AAAAAAAAAAATTAACTAAACTTA	ab
+1	AAAAAAAAAACAAATTCTATTATT	ab
+1	AAAAAAAAAACTCCCAGATTTTTT	ab
+1	AAAAAAAAAACTTCTGCTTGGCGG	ba
+11	AAAAAAAAAAGAATCGTGGTTTGT	ba
+5	AAAAAAAAAAGATAGCCCTAAACG	ab
+1	AAAAAAAAAAGCAATAATGCCAGT	ab
+2	AAAAAAAAAAGTACCGCACTCTCA	ba
+1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
+1	AAAAAAAAAATAACTTCAATAATG	ba
+2	AAAAAAAAAATAATCATAGACTCT	ab
+1	AAAAAAAAAATAGTCTCACATTTA	ab
+1	AAAAAAAAAATATAACCTTTGGCG	ab
+3	AAAAAAAAACAAAATTCTATTATT	ab
+1	AAAAAAAAACAAGTACGCGGCATT	ab
+1	AAAAAAAAACAAGTACGCGGTATT	ab
+1	AAAAAAAAACAATATCGAATTAAC	ab
+3	AAAAAAAAACACGGTGAGACAAGG	ba
+1	AAAAAAAAACACGTTTCTCCCCTT	ba
+1	AAAAAAAAACATATCGTCCCGAGC	ba
+1	AAAAAAAAACCTACCTGAGGCCCC	ab
+3	AAAAAAAAACCTTATTACAGCGGA	ab
+1	AAAAAAAAACGATTCTCTGTATCT	ba
+1	AAAAAAAAACGTACCGCACTCTCA	ba
+4	AAAAAAAAACTACCCAGATTTTTT	ba
+1	AAAAAAAAACTAGATGAGACGACC	ba
+4	AAAAAAAAACTGTCTGCTTGGCGG	ba
+1	AAAAAAAAAGAAGTTTAATTTTAA	ab
+1	AAAAAAAAAGAATGCCTAAGACGA	ba
+6	AAAAAAAAAGACCGGCCTTAGACA	ba
+1	AAAAAAAAAGATATCGTGGTTTGT	ba
+1	AAAAAAAAAGCAATACTCAAGCTG	ba
+6	AAAAAAAAAGCAATGTCTAAGCCT	ba
+1	AAAAAAAAAGCACTGTCTAAGCCT	ab
+2	AAAAAAAAAGCTAATAATGCCAGT	ab
+1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
+1	AAAAAAAAATAAAGGTCCGAATCT	ab
+1	AAAAAAAAATAAATGAGAGTGTAA	ba
+8	AAAAAAAAATAAGTCTCACATTTA	ab
+1	AAAAAAAAATAATAACCTCTGGCG	ab
+10	AAAAAAAAATAATAACCTTTGGCG	ab
+1	AAAAAAAAATAATCCCCTTTGTCG	ab
+6	AAAAAAAAATACGCAAACGCTGAG	ab
+4	AAAAAAAAATAGATCATAGACTCT	ab
+10	AAAAAAAAATAGATCATAGACTCT	ba
+10	AAAAAAAAATAGTAGGATTTCATG	ba
+7	AAAAAAAAATATGAATACCCTCGT	ba
+1	AAAAAAAAATATGCCACTTGATCC	ba
+1	AAAAAAAAATATTCTGCCACTTGA	ba
+3	AAAAAAAAATCAAACCAAGAGGAC	ba
+1	AAAAAAAAATCAGTACCCCTAAAC	ab
+12	AAAAAAAAATCCTAGTTAATGAAG	ba
+1	AAAAAAAAATCGATTCTTTATGCG	ab
+1	AAAAAAAAATGTCTGAAAATATCT	ab
+4	AAAAAAAAATGTCTGAAAATATCT	ba
+1	AAAAAAAAATTTCCGCAGACCGTT	ba
+8	AAAAAAAAATTTGGGCTACTACAA	ba
+1	AAAAAAAACAAAATTAGAACCCTT	ab
+1	AAAAAAAACAAACCGCTCCTCACA	ba
+5	AAAAAAAACAACGTACGCGGTATT	ab
+4	AAAAAAAACAATATCGTTGATATG	ba
+4	AAAAAAAACAATCACGTTAATAGG	ab
+1	AAAAAAAACAGAATCGTGGTTTGT	ba
+1	AAAAAAAACCAAATCGTTGATATG	ba
+9	AAAAAAAACCAAGTCCAGGCATCT	ba
+2	AAAAAAAACCACGGTGAGACAAGG	ba
+1	AAAAAAAACCGCCCAACTGCCGGT	ab
+5	AAAAAAAACCTCTCAACCCCAAAT	ba
+7	AAAAAAAACCTCTTGCGATGTTGT	ab
+1	AAAAAAAACCTCTTGCGCTGTTGT	ab
+1	AAAAAAAACCTCTTGTGATGTTGT	ab
+12	AAAAAAAACCTGAGCAATGGTTCC	ab
+3	AAAAAAAACCTTGACCCTCACATG	ba
+6	AAAAAAAACCTTGCACTCGTCCTA	ba
+9	AAAAAAAACGAAATAAAAAAACCT	ba
+1	AAAAAAAACGACCGGCCTTAGACA	ba
+4	AAAAAAAACGCCACCACCCCCTTT	ab
+12	AAAAAAAACGCCACGGGCACTATT	ba
+13	AAAAAAAACGTATCAGTAGATCCT	ab
+1	AAAAAAAACTAGTAGGATTTCATG	ba
+3	AAAAAAAACTATAGAAAATCCATT	ba
+1	AAAAAAAACTATTCTATTTCCGAT	ba
+13	AAAAAAAACTGATCTGCTTGGCGG	ba
+8	AAAAAAAACTTGCGAATAGCATCG	ba
+4	AAAAAAAACTTGTTATCAAAACGT	ab
+1	AAAAAAAAGAAAAGTTCAACACGC	ba
+1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
+13	AAAAAAAAGAGAGTTTAGTCATGG	ab
+1	AAAAAAAAGAGAGTTTAGTCATGG	ba
+1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_data2.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,112 @@
+1	AAAAAAAAAAAAAACCAAAACTTC	ba
+1	AAAAAAAAAAAAACCAGGCGTCGA	ba
+1	AAAAAAAAAAAAAGCTCCACGTTG	ba
+1	AAAAAAAAAAAAATCGTGGTTTGT	ba
+1	AAAAAAAAAAAAATTCACCCTTGT	ba
+7	AAAAAAAAAAAACACACTTAACTT	ba
+1	AAAAAAAAAAAACAGTGTTGAGAC	ba
+4	AAAAAAAAAAAACCGCTCCTCACA	ba
+1	AAAAAAAAAAAAGGCAACACAGAA	ab
+2	AAAAAAAAAAAATCTTTCTTTGAG	ab
+1	AAAAAAAAAAAATTGGGTTCCTTA	ab
+1	AAAAAAAAAAAGAGTCGCACCCAG	ba
+21	AAAAAAAAAAAGATCGTGGTTTGT	ba
+1	AAAAAAAAAAAGCGCAACACAGAA	ab
+3	AAAAAAAAAAAGGGCAACACAGAA	ab
+1	AAAAAAAAAAAGTAGCCCTAAACG	ab
+1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
+1	AAAAAAAAAAATATCATAGACTCT	ab
+6	AAAAAAAAAAATATTCACCCTTGT	ba
+1	AAAAAAAAAAATATTCGAAAGTTA	ba
+3	AAAAAAAAAAATCACACTTAACTT	ba
+1	AAAAAAAAAAATCCGCTCCTCACA	ba
+1	AAAAAAAAAAATTAACTAAACTTA	ab
+1	AAAAAAAAAACAAATTCTATTATT	ab
+1	AAAAAAAAAACTCCCAGATTTTTT	ab
+1	AAAAAAAAAACTTCTGCTTGGCGG	ba
+11	AAAAAAAAAAGAATCGTGGTTTGT	ba
+5	AAAAAAAAAAGATAGCCCTAAACG	ab
+1	AAAAAAAAAAGCAATAATGCCAGT	ab
+2	AAAAAAAAAAGTACCGCACTCTCA	ba
+1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
+1	AAAAAAAAAATAACTTCAATAATG	ba
+2	AAAAAAAAAATAATCATAGACTCT	ab
+1	AAAAAAAAAATAGTCTCACATTTA	ab
+1	AAAAAAAAAATATAACCTTTGGCG	ab
+3	AAAAAAAAACAAAATTCTATTATT	ab
+1	AAAAAAAAACAAGTACGCGGCATT	ab
+1	AAAAAAAAACAAGTACGCGGTATT	ab
+1	AAAAAAAAACAATATCGAATTAAC	ab
+3	AAAAAAAAACACGGTGAGACAAGG	ba
+1	AAAAAAAAACACGTTTCTCCCCTT	ba
+1	AAAAAAAAACATATCGTCCCGAGC	ba
+1	AAAAAAAAACCTACCTGAGGCCCC	ab
+3	AAAAAAAAACCTTATTACAGCGGA	ab
+1	AAAAAAAAACGATTCTCTGTATCT	ba
+1	AAAAAAAAACGTACCGCACTCTCA	ba
+4	AAAAAAAAACTACCCAGATTTTTT	ba
+1	AAAAAAAAACTAGATGAGACGACC	ba
+4	AAAAAAAAACTGTCTGCTTGGCGG	ba
+1	AAAAAAAAAGAAGTTTAATTTTAA	ab
+1	AAAAAAAAAGAATGCCTAAGACGA	ba
+6	AAAAAAAAAGACCGGCCTTAGACA	ba
+1	AAAAAAAAAGATATCGTGGTTTGT	ba
+1	AAAAAAAAAGCAATACTCAAGCTG	ba
+6	AAAAAAAAAGCAATGTCTAAGCCT	ba
+1	AAAAAAAAAGCACTGTCTAAGCCT	ab
+2	AAAAAAAAAGCTAATAATGCCAGT	ab
+1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
+1	AAAAAAAAATAAAGGTCCGAATCT	ab
+1	AAAAAAAAATAAATGAGAGTGTAA	ba
+8	AAAAAAAAATAAGTCTCACATTTA	ab
+1	AAAAAAAAATAATAACCTCTGGCG	ab
+10	AAAAAAAAATAATAACCTTTGGCG	ab
+1	AAAAAAAAATAATCCCCTTTGTCG	ab
+6	AAAAAAAAATACGCAAACGCTGAG	ab
+4	AAAAAAAAATAGATCATAGACTCT	ab
+10	AAAAAAAAATAGATCATAGACTCT	ba
+10	AAAAAAAAATAGTAGGATTTCATG	ba
+7	AAAAAAAAATATGAATACCCTCGT	ba
+1	AAAAAAAAATATGCCACTTGATCC	ba
+1	AAAAAAAAATATTCTGCCACTTGA	ba
+3	AAAAAAAAATCAAACCAAGAGGAC	ba
+1	AAAAAAAAATCAGTACCCCTAAAC	ab
+12	AAAAAAAAATCCTAGTTAATGAAG	ba
+1	AAAAAAAAATCGATTCTTTATGCG	ab
+1	AAAAAAAAATGTCTGAAAATATCT	ab
+4	AAAAAAAAATGTCTGAAAATATCT	ba
+1	AAAAAAAAATTTCCGCAGACCGTT	ba
+8	AAAAAAAAATTTGGGCTACTACAA	ba
+1	AAAAAAAACAAAATTAGAACCCTT	ab
+1	AAAAAAAACAAACCGCTCCTCACA	ba
+5	AAAAAAAACAACGTACGCGGTATT	ab
+4	AAAAAAAACAATATCGTTGATATG	ba
+4	AAAAAAAACAATCACGTTAATAGG	ab
+1	AAAAAAAACAGAATCGTGGTTTGT	ba
+1	AAAAAAAACCAAATCGTTGATATG	ba
+9	AAAAAAAACCAAGTCCAGGCATCT	ba
+2	AAAAAAAACCACGGTGAGACAAGG	ba
+1	AAAAAAAACCGCCCAACTGCCGGT	ab
+5	AAAAAAAACCTCTCAACCCCAAAT	ba
+7	AAAAAAAACCTCTTGCGATGTTGT	ab
+1	AAAAAAAACCTCTTGCGCTGTTGT	ab
+1	AAAAAAAACCTCTTGTGATGTTGT	ab
+12	AAAAAAAACCTGAGCAATGGTTCC	ab
+3	AAAAAAAACCTTGACCCTCACATG	ba
+6	AAAAAAAACCTTGCACTCGTCCTA	ba
+9	AAAAAAAACGAAATAAAAAAACCT	ba
+1	AAAAAAAACGACCGGCCTTAGACA	ba
+4	AAAAAAAACGCCACCACCCCCTTT	ab
+12	AAAAAAAACGCCACGGGCACTATT	ba
+13	AAAAAAAACGTATCAGTAGATCCT	ab
+1	AAAAAAAACTAGTAGGATTTCATG	ba
+3	AAAAAAAACTATAGAAAATCCATT	ba
+1	AAAAAAAACTATTCTATTTCCGAT	ba
+13	AAAAAAAACTGATCTGCTTGGCGG	ba
+8	AAAAAAAACTTGCGAATAGCATCG	ba
+4	AAAAAAAACTTGTTATCAAAACGT	ab
+1	AAAAAAAAGAAAAGTTCAACACGC	ba
+1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
+13	AAAAAAAAGAGAGTTTAGTCATGG	ab
+1	AAAAAAAAGAGAGTTTAGTCATGG	ba
+1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_data3.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,112 @@
+1	AAAAAAAAAAAAAACCAAAACTTC	ba
+1	AAAAAAAAAAAAACCAGGCGTCGA	ba
+1	AAAAAAAAAAAAAGCTCCACGTTG	ba
+1	AAAAAAAAAAAAATCGTGGTTTGT	ba
+1	AAAAAAAAAAAAATTCACCCTTGT	ba
+7	AAAAAAAAAAAACACACTTAACTT	ba
+1	AAAAAAAAAAAACAGTGTTGAGAC	ba
+4	AAAAAAAAAAAACCGCTCCTCACA	ba
+1	AAAAAAAAAAAAGGCAACACAGAA	ab
+2	AAAAAAAAAAAATCTTTCTTTGAG	ab
+1	AAAAAAAAAAAATTGGGTTCCTTA	ab
+1	AAAAAAAAAAAGAGTCGCACCCAG	ba
+21	AAAAAAAAAAAGATCGTGGTTTGT	ba
+1	AAAAAAAAAAAGCGCAACACAGAA	ab
+3	AAAAAAAAAAAGGGCAACACAGAA	ab
+1	AAAAAAAAAAAGTAGCCCTAAACG	ab
+1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
+1	AAAAAAAAAAATATCATAGACTCT	ab
+6	AAAAAAAAAAATATTCACCCTTGT	ba
+1	AAAAAAAAAAATATTCGAAAGTTA	ba
+3	AAAAAAAAAAATCACACTTAACTT	ba
+1	AAAAAAAAAAATCCGCTCCTCACA	ba
+1	AAAAAAAAAAATTAACTAAACTTA	ab
+1	AAAAAAAAAACAAATTCTATTATT	ab
+1	AAAAAAAAAACTCCCAGATTTTTT	ab
+1	AAAAAAAAAACTTCTGCTTGGCGG	ba
+11	AAAAAAAAAAGAATCGTGGTTTGT	ba
+5	AAAAAAAAAAGATAGCCCTAAACG	ab
+1	AAAAAAAAAAGCAATAATGCCAGT	ab
+2	AAAAAAAAAAGTACCGCACTCTCA	ba
+1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
+1	AAAAAAAAAATAACTTCAATAATG	ba
+2	AAAAAAAAAATAATCATAGACTCT	ab
+1	AAAAAAAAAATAGTCTCACATTTA	ab
+1	AAAAAAAAAATATAACCTTTGGCG	ab
+3	AAAAAAAAACAAAATTCTATTATT	ab
+1	AAAAAAAAACAAGTACGCGGCATT	ab
+1	AAAAAAAAACAAGTACGCGGTATT	ab
+1	AAAAAAAAACAATATCGAATTAAC	ab
+3	AAAAAAAAACACGGTGAGACAAGG	ba
+1	AAAAAAAAACACGTTTCTCCCCTT	ba
+1	AAAAAAAAACATATCGTCCCGAGC	ba
+1	AAAAAAAAACCTACCTGAGGCCCC	ab
+3	AAAAAAAAACCTTATTACAGCGGA	ab
+1	AAAAAAAAACGATTCTCTGTATCT	ba
+1	AAAAAAAAACGTACCGCACTCTCA	ba
+4	AAAAAAAAACTACCCAGATTTTTT	ba
+1	AAAAAAAAACTAGATGAGACGACC	ba
+4	AAAAAAAAACTGTCTGCTTGGCGG	ba
+1	AAAAAAAAAGAAGTTTAATTTTAA	ab
+1	AAAAAAAAAGAATGCCTAAGACGA	ba
+6	AAAAAAAAAGACCGGCCTTAGACA	ba
+1	AAAAAAAAAGATATCGTGGTTTGT	ba
+1	AAAAAAAAAGCAATACTCAAGCTG	ba
+6	AAAAAAAAAGCAATGTCTAAGCCT	ba
+1	AAAAAAAAAGCACTGTCTAAGCCT	ab
+2	AAAAAAAAAGCTAATAATGCCAGT	ab
+1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
+1	AAAAAAAAATAAAGGTCCGAATCT	ab
+1	AAAAAAAAATAAATGAGAGTGTAA	ba
+8	AAAAAAAAATAAGTCTCACATTTA	ab
+1	AAAAAAAAATAATAACCTCTGGCG	ab
+10	AAAAAAAAATAATAACCTTTGGCG	ab
+1	AAAAAAAAATAATCCCCTTTGTCG	ab
+6	AAAAAAAAATACGCAAACGCTGAG	ab
+4	AAAAAAAAATAGATCATAGACTCT	ab
+10	AAAAAAAAATAGATCATAGACTCT	ba
+10	AAAAAAAAATAGTAGGATTTCATG	ba
+7	AAAAAAAAATATGAATACCCTCGT	ba
+1	AAAAAAAAATATGCCACTTGATCC	ba
+1	AAAAAAAAATATTCTGCCACTTGA	ba
+3	AAAAAAAAATCAAACCAAGAGGAC	ba
+1	AAAAAAAAATCAGTACCCCTAAAC	ab
+12	AAAAAAAAATCCTAGTTAATGAAG	ba
+1	AAAAAAAAATCGATTCTTTATGCG	ab
+1	AAAAAAAAATGTCTGAAAATATCT	ab
+4	AAAAAAAAATGTCTGAAAATATCT	ba
+1	AAAAAAAAATTTCCGCAGACCGTT	ba
+8	AAAAAAAAATTTGGGCTACTACAA	ba
+1	AAAAAAAACAAAATTAGAACCCTT	ab
+1	AAAAAAAACAAACCGCTCCTCACA	ba
+5	AAAAAAAACAACGTACGCGGTATT	ab
+4	AAAAAAAACAATATCGTTGATATG	ba
+4	AAAAAAAACAATCACGTTAATAGG	ab
+1	AAAAAAAACAGAATCGTGGTTTGT	ba
+1	AAAAAAAACCAAATCGTTGATATG	ba
+9	AAAAAAAACCAAGTCCAGGCATCT	ba
+2	AAAAAAAACCACGGTGAGACAAGG	ba
+1	AAAAAAAACCGCCCAACTGCCGGT	ab
+5	AAAAAAAACCTCTCAACCCCAAAT	ba
+7	AAAAAAAACCTCTTGCGATGTTGT	ab
+1	AAAAAAAACCTCTTGCGCTGTTGT	ab
+1	AAAAAAAACCTCTTGTGATGTTGT	ab
+12	AAAAAAAACCTGAGCAATGGTTCC	ab
+3	AAAAAAAACCTTGACCCTCACATG	ba
+6	AAAAAAAACCTTGCACTCGTCCTA	ba
+9	AAAAAAAACGAAATAAAAAAACCT	ba
+1	AAAAAAAACGACCGGCCTTAGACA	ba
+4	AAAAAAAACGCCACCACCCCCTTT	ab
+12	AAAAAAAACGCCACGGGCACTATT	ba
+13	AAAAAAAACGTATCAGTAGATCCT	ab
+1	AAAAAAAACTAGTAGGATTTCATG	ba
+3	AAAAAAAACTATAGAAAATCCATT	ba
+1	AAAAAAAACTATTCTATTTCCGAT	ba
+13	AAAAAAAACTGATCTGCTTGGCGG	ba
+8	AAAAAAAACTTGCGAATAGCATCG	ba
+4	AAAAAAAACTTGTTATCAAAACGT	ab
+1	AAAAAAAAGAAAAGTTCAACACGC	ba
+1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
+13	AAAAAAAAGAGAGTTTAGTCATGG	ab
+1	AAAAAAAAGAGAGTTTAGTCATGG	ba
+1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_data4.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,112 @@
+1	AAAAAAAAAAAAAACCAAAACTTC	ba
+1	AAAAAAAAAAAAACCAGGCGTCGA	ba
+1	AAAAAAAAAAAAAGCTCCACGTTG	ba
+1	AAAAAAAAAAAAATCGTGGTTTGT	ba
+1	AAAAAAAAAAAAATTCACCCTTGT	ba
+7	AAAAAAAAAAAACACACTTAACTT	ba
+1	AAAAAAAAAAAACAGTGTTGAGAC	ba
+4	AAAAAAAAAAAACCGCTCCTCACA	ba
+1	AAAAAAAAAAAAGGCAACACAGAA	ab
+2	AAAAAAAAAAAATCTTTCTTTGAG	ab
+1	AAAAAAAAAAAATTGGGTTCCTTA	ab
+1	AAAAAAAAAAAGAGTCGCACCCAG	ba
+21	AAAAAAAAAAAGATCGTGGTTTGT	ba
+1	AAAAAAAAAAAGCGCAACACAGAA	ab
+3	AAAAAAAAAAAGGGCAACACAGAA	ab
+1	AAAAAAAAAAAGTAGCCCTAAACG	ab
+1	AAAAAAAAAAAGTCTTTCTTTGAG	ab
+1	AAAAAAAAAAATATCATAGACTCT	ab
+6	AAAAAAAAAAATATTCACCCTTGT	ba
+1	AAAAAAAAAAATATTCGAAAGTTA	ba
+3	AAAAAAAAAAATCACACTTAACTT	ba
+1	AAAAAAAAAAATCCGCTCCTCACA	ba
+1	AAAAAAAAAAATTAACTAAACTTA	ab
+1	AAAAAAAAAACAAATTCTATTATT	ab
+1	AAAAAAAAAACTCCCAGATTTTTT	ab
+1	AAAAAAAAAACTTCTGCTTGGCGG	ba
+11	AAAAAAAAAAGAATCGTGGTTTGT	ba
+5	AAAAAAAAAAGATAGCCCTAAACG	ab
+1	AAAAAAAAAAGCAATAATGCCAGT	ab
+2	AAAAAAAAAAGTACCGCACTCTCA	ba
+1	AAAAAAAAAAGTTCTTTCTTTGAG	ab
+1	AAAAAAAAAATAACTTCAATAATG	ba
+2	AAAAAAAAAATAATCATAGACTCT	ab
+1	AAAAAAAAAATAGTCTCACATTTA	ab
+1	AAAAAAAAAATATAACCTTTGGCG	ab
+3	AAAAAAAAACAAAATTCTATTATT	ab
+1	AAAAAAAAACAAGTACGCGGCATT	ab
+1	AAAAAAAAACAAGTACGCGGTATT	ab
+1	AAAAAAAAACAATATCGAATTAAC	ab
+3	AAAAAAAAACACGGTGAGACAAGG	ba
+1	AAAAAAAAACACGTTTCTCCCCTT	ba
+1	AAAAAAAAACATATCGTCCCGAGC	ba
+1	AAAAAAAAACCTACCTGAGGCCCC	ab
+3	AAAAAAAAACCTTATTACAGCGGA	ab
+1	AAAAAAAAACGATTCTCTGTATCT	ba
+1	AAAAAAAAACGTACCGCACTCTCA	ba
+4	AAAAAAAAACTACCCAGATTTTTT	ba
+1	AAAAAAAAACTAGATGAGACGACC	ba
+4	AAAAAAAAACTGTCTGCTTGGCGG	ba
+1	AAAAAAAAAGAAGTTTAATTTTAA	ab
+1	AAAAAAAAAGAATGCCTAAGACGA	ba
+6	AAAAAAAAAGACCGGCCTTAGACA	ba
+1	AAAAAAAAAGATATCGTGGTTTGT	ba
+1	AAAAAAAAAGCAATACTCAAGCTG	ba
+6	AAAAAAAAAGCAATGTCTAAGCCT	ba
+1	AAAAAAAAAGCACTGTCTAAGCCT	ab
+2	AAAAAAAAAGCTAATAATGCCAGT	ab
+1	AAAAAAAAAGTTTCGTGAAGGTCC	ba
+1	AAAAAAAAATAAAGGTCCGAATCT	ab
+1	AAAAAAAAATAAATGAGAGTGTAA	ba
+8	AAAAAAAAATAAGTCTCACATTTA	ab
+1	AAAAAAAAATAATAACCTCTGGCG	ab
+10	AAAAAAAAATAATAACCTTTGGCG	ab
+1	AAAAAAAAATAATCCCCTTTGTCG	ab
+6	AAAAAAAAATACGCAAACGCTGAG	ab
+4	AAAAAAAAATAGATCATAGACTCT	ab
+10	AAAAAAAAATAGATCATAGACTCT	ba
+10	AAAAAAAAATAGTAGGATTTCATG	ba
+7	AAAAAAAAATATGAATACCCTCGT	ba
+1	AAAAAAAAATATGCCACTTGATCC	ba
+1	AAAAAAAAATATTCTGCCACTTGA	ba
+3	AAAAAAAAATCAAACCAAGAGGAC	ba
+1	AAAAAAAAATCAGTACCCCTAAAC	ab
+12	AAAAAAAAATCCTAGTTAATGAAG	ba
+1	AAAAAAAAATCGATTCTTTATGCG	ab
+1	AAAAAAAAATGTCTGAAAATATCT	ab
+4	AAAAAAAAATGTCTGAAAATATCT	ba
+1	AAAAAAAAATTTCCGCAGACCGTT	ba
+8	AAAAAAAAATTTGGGCTACTACAA	ba
+1	AAAAAAAACAAAATTAGAACCCTT	ab
+1	AAAAAAAACAAACCGCTCCTCACA	ba
+5	AAAAAAAACAACGTACGCGGTATT	ab
+4	AAAAAAAACAATATCGTTGATATG	ba
+4	AAAAAAAACAATCACGTTAATAGG	ab
+1	AAAAAAAACAGAATCGTGGTTTGT	ba
+1	AAAAAAAACCAAATCGTTGATATG	ba
+9	AAAAAAAACCAAGTCCAGGCATCT	ba
+2	AAAAAAAACCACGGTGAGACAAGG	ba
+1	AAAAAAAACCGCCCAACTGCCGGT	ab
+5	AAAAAAAACCTCTCAACCCCAAAT	ba
+7	AAAAAAAACCTCTTGCGATGTTGT	ab
+1	AAAAAAAACCTCTTGCGCTGTTGT	ab
+1	AAAAAAAACCTCTTGTGATGTTGT	ab
+12	AAAAAAAACCTGAGCAATGGTTCC	ab
+3	AAAAAAAACCTTGACCCTCACATG	ba
+6	AAAAAAAACCTTGCACTCGTCCTA	ba
+9	AAAAAAAACGAAATAAAAAAACCT	ba
+1	AAAAAAAACGACCGGCCTTAGACA	ba
+4	AAAAAAAACGCCACCACCCCCTTT	ab
+12	AAAAAAAACGCCACGGGCACTATT	ba
+13	AAAAAAAACGTATCAGTAGATCCT	ab
+1	AAAAAAAACTAGTAGGATTTCATG	ba
+3	AAAAAAAACTATAGAAAATCCATT	ba
+1	AAAAAAAACTATTCTATTTCCGAT	ba
+13	AAAAAAAACTGATCTGCTTGGCGG	ba
+8	AAAAAAAACTTGCGAATAGCATCG	ba
+4	AAAAAAAACTTGTTATCAAAACGT	ab
+1	AAAAAAAAGAAAAGTTCAACACGC	ba
+1	AAAAAAAAGAAGTTCGCCCTCCGA	ab
+13	AAAAAAAAGAGAGTTTAGTCATGG	ab
+1	AAAAAAAAGAGAGTTTAGTCATGG	ba
+1	AAAAAAAAGAGAGTTTAGTCCTGG	ab
\ No newline at end of file
Binary file test-data/fsd_output1.pdf has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_output1.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,224 @@
+Values from family size distribution with all datasets
+
+Family size	fsd_data1.tab	fsd_data2.tab	fsd_data3.tab	fsd_data4.tab
+FS=1	63	63	63	63	
+FS=2	5	5	5	5	
+FS=3	8	8	8	8	
+FS=4	9	9	9	9	
+FS=5	3	3	3	3	
+FS=6	5	5	5	5	
+FS=7	3	3	3	3	
+FS=8	3	3	3	3	
+FS=9	2	2	2	2	
+FS=10	3	3	3	3	
+FS=11	1	1	1	1	
+FS=12	3	3	3	3	
+FS=13	3	3	3	3	
+FS=14	0	0	0	0	
+FS=15	0	0	0	0	
+FS=16	0	0	0	0	
+FS=17	0	0	0	0	
+FS=18	0	0	0	0	
+FS=19	0	0	0	0	
+FS=20	0	0	0	0	
+FS>20	1	1	1	1	
+sum	112	112	112	112	
+Dataset:	fsd_data1.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data1.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
+
+Dataset:	fsd_data2.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data2.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
+
+Dataset:	fsd_data3.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data3.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
+
+Dataset:	fsd_data4.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data4.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
Binary file test-data/fsd_output2.pdf has changed
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/test-data/fsd_output2.tab	Tue Apr 02 05:10:09 2019 -0400
@@ -0,0 +1,174 @@
+Values from family size distribution with all datasets
+
+Family size	fsd_data1.tab	fsd_data2.tab	fsd_data3.tab
+FS=1	63	63	63	
+FS=2	5	5	5	
+FS=3	8	8	8	
+FS=4	9	9	9	
+FS=5	3	3	3	
+FS=6	5	5	5	
+FS=7	3	3	3	
+FS=8	3	3	3	
+FS=9	2	2	2	
+FS=10	3	3	3	
+FS=11	1	1	1	
+FS=12	3	3	3	
+FS=13	3	3	3	
+FS=14	0	0	0	
+FS=15	0	0	0	
+FS=16	0	0	0	
+FS=17	0	0	0	
+FS=18	0	0	0	
+FS=19	0	0	0	
+FS=20	0	0	0	
+FS>20	1	1	1	
+sum	112	112	112	
+Dataset:	fsd_data1.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data1.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
+
+Dataset:	fsd_data2.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data2.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
+
+Dataset:	fsd_data3.tab
+max. family size:	21
+absolute frequency:	1
+relative frequency:	0.009
+
+	singletons:			family size > 20:
+	nr. of tags	rel. freq of tags	rel.freq of PE reads	nr. of tags	rel. freq of tags	nr. of PE reads	rel. freq of PE reads	total nr. of tags	total nr. of PE reads
+fsd_data3.tab	63	0.562	0.167	1	0.009	21	0.056	112	378
+
+The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
+Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
+
+FS >= 1	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	47	123	0.431	0.339	0.420	0.325
+SSCS ba	59	222	0.488	0.481	0.476	0.468
+DCS (total)	3 (6)	18 (33)	0.028	0.027 (0.054)	0.050	0.048 (0.087)
+total nr. of tags	109	363	109	112	363	378
+
+FS >= 3	nr. of tags	nr. of PE reads	rel. freq of tags		rel. freq of PE reads:
+			unique:	total	unique	total:
+SSCS ab	14	87	0.341	0.259	0.313	0.224
+SSCS ba	26	187	0.491	0.481	0.495	0.482
+DCS (total)	1 (2)	4 (14)	0.024	0.024 (0.048)	0.014	0.014 (0.049)
+total nr. of tags	41	278	41	42	278	288
+
+Values from family size distribution
+	duplex	ab	ba	sum
+FS=1	2	30	31	63
+FS=2	0	3	2	5
+FS=3	0	3	5	8
+FS=4	2	3	4	9
+FS=5	0	2	1	3
+FS=6	0	1	4	5
+FS=7	0	1	2	3
+FS=8	0	1	2	3
+FS=9	0	0	2	2
+FS=10	1	1	1	3
+FS=11	0	0	1	1
+FS=12	0	1	2	3
+FS=13	1	1	1	3
+FS=14	0	0	0	0
+FS=15	0	0	0	0
+FS=16	0	0	0	0
+FS=17	0	0	0	0
+FS=18	0	0	0	0
+FS=19	0	0	0	0
+FS=20	0	0	0	0
+FS>20	0	0	1	1
Binary file test-data/output_file.pdf has changed
--- a/test-data/output_file.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,167 +0,0 @@
-Values from family size distribution with all datasets
-
-Family size	Test_data	Test_data2	Test_data3	Test_data4
-FS=1	63	63	63	63	
-FS=2	5	5	5	5	
-FS=3	8	8	8	8	
-FS=4	10	10	10	10	
-FS=5	3	3	3	3	
-FS=6	5	5	5	5	
-FS=7	3	3	3	3	
-FS=8	3	3	3	3	
-FS=9	2	2	2	2	
-FS=10	3	3	3	3	
-FS=11	1	1	1	1	
-FS=12	6	6	6	6	
-sum	112	112	112	112	
-Dataset:	Test_data
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
-
-Dataset:	Test_data2
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data2	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
-
-Dataset:	Test_data3
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data3	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
-
-Dataset:	Test_data4
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data4	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
Binary file test-data/output_file2.pdf has changed
--- a/test-data/output_file2.tabular	Mon Oct 08 05:50:18 2018 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,129 +0,0 @@
-Values from family size distribution with all datasets
-
-Family size	Test_data	Test_data2	Test_data3
-FS=1	63	63	63	
-FS=2	5	5	5	
-FS=3	8	8	8	
-FS=4	10	10	10	
-FS=5	3	3	3	
-FS=6	5	5	5	
-FS=7	3	3	3	
-FS=8	3	3	3	
-FS=9	2	2	2	
-FS=10	3	3	3	
-FS=11	1	1	1	
-FS=12	6	6	6	
-sum	112	112	112	
-Dataset:	Test_data
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
-
-Dataset:	Test_data2
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data2	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
-
-Dataset:	Test_data3
-max. family size:	13
-absolute frequency:	3
-relative frequency:	0.027
-
-	singletons:		family size > 20:
-	absolute nr.	rel. freq	absolute nr.	rel. freq	total length
-Test_data3	63	0.562	6	0.054	112
-
-The unique frequencies were calculated from the dataset where the tags occured only once (=ab without DCS, ba without DCS)
-Whereas the total frequencies were calculated from the whole dataset (=including the DCS).
-
-FS >= 1		unique:	total:
-nr./rel. freq of ab=	47	0.431	0.420
-nr./rel. freq of ba=	59	0.488	0.476
-nr./rel. freq of DCS (total)=	3 (6)	0.028	0.027 (0.054)
-length of dataset=	109	109	112
-FS >= 3		unique:	total:
-nr./rel. freq of ab=	14	0.341	0.318
-nr./rel. freq of ba=	26	0.491	0.464
-nr./rel. freq of DCS (total)=	1 (2)	0.037	0.023 (0.045)
-length of dataset=	41	41	44
-
-Values from family size distribution
-	duplex	ab	ba	sum
-FS=1	2	30	31	63
-FS=2	0	3	2	5
-FS=3	0	3	5	8
-FS=4	2	3	5	10
-FS=5	0	2	1	3
-FS=6	0	1	4	5
-FS=7	0	1	2	3
-FS=8	0	1	2	3
-FS=9	0	0	2	2
-FS=10	1	1	1	3
-FS=11	0	0	1	1
-FS=12	1	2	3	6
PKEZOUD|4 mothur_chimera_pintail-93b61db8bdb6/.hg_archival.txtUT]PKEZO[i~ * mothur_chimera_pintail-93b61db8bdb6/READMEUT]PKEZO0~D %7 mothur_chimera_pintail-93b61db8bdb6/chimera.pintail.xmlUT]PKEZOwi;o:. xmothur_chimera_pintail-93b61db8bdb6/macros.xmlUT]PKEZOztqVTF L!mothur_chimera_pintail-93b61db8bdb6/test-data/98_sq_phylip_amazon.distUT]PKEZOu D mothur_chimera_pintail-93b61db8bdb6/test-data/Fasting_Example1.fastaUT]PKEZOR C mothur_chimera_pintail-93b61db8bdb6/test-data/Fasting_Example1.qualUT]PKEZOD\ZAH wmothur_chimera_pintail-93b61db8bdb6/test-data/Fasting_Example1.scrap.sffUT]PKP