annotate test-data/output_file.tabular @ 24:3bc67ac46740 draft

planemo upload for repository https://github.com/monikaheinzl/duplexanalysis_galaxy/tree/master/tools/hd commit b8a2f7b7615b2bcd3b602027af31f4e677da94f6-dirty

Test_data
number of tags per file	20 (from 20) against 20

Hamming distance separated by family size
	FS=1	FS=2	FS=3	FS=4	FS=5-10	FS>10	sum	
HD=1	5	1	1	1	1	0	9	
HD=6	3	0	0	0	0	0	3	
HD=7	4	0	0	0	1	0	5	
HD=8	2	0	0	1	0	0	3	
sum	14	1	1	2	2	0	20	

Family size distribution separated by Hamming distance
	HD=1	HD=2	HD=3	HD=4	HD=5-8	HD>8	sum	
FS=1	5	0	0	0	9	0	14	
FS=2	1	0	0	0	0	0	1	
FS=3	1	0	0	0	0	0	1	
FS=4	1	0	0	0	1	0	2	
FS=6	1	0	0	0	0	0	1	
FS=7	0	0	0	0	1	0	1	
sum	9	0	0	0	11	0	20	


max. family size in sample:	7
absolute frequency:	1
relative frequency:	0.05

The hamming distances were calculated by comparing each half of all tags against the tag(s) with the minimum Hamming distance per half.
Since this calculation was repeated, but starting with the second half to find all possible chimeras in the data, the actual number of tags in the plots differs from the sample size entered by the user.
In addition, both family sizes of one tag will be included in the plots if only tags of reads that can form a DCS were allowed.
length of one part of the tag = 12

Hamming distance of each half in the tag
	HD a	HD b'	HD b	HD a'	HD a+b	sum	
HD=0	20	0	8	1	0	29	
HD=1	0	0	1	19	8	28	
HD=2	0	0	0	0	1	1	
HD=5	0	0	3	0	0	3	
HD=6	0	0	2	0	3	5	
HD=7	0	1	6	0	4	11	
HD=8	0	2	0	0	7	9	
HD=9	0	1	0	0	1	2	
HD=10	0	2	0	0	2	4	
HD=11	0	7	0	0	7	14	
HD=12	0	7	0	0	7	14	
sum	20	20	20	20	40	120	

Absolute delta Hamming distances within the tag
	FS=1	FS=2	FS=3	FS=4	FS=5-10	FS>10	sum	
diff=0	1	0	0	0	0	0	1	
diff=1	4	1	1	1	1	0	8	
diff=4	3	0	0	0	0	0	3	
diff=5	2	0	0	0	0	0	2	
diff=6	3	0	0	1	1	0	5	
diff=7	2	0	0	0	0	0	2	
diff=8	1	0	0	0	1	0	2	
diff=9	1	0	0	0	0	0	1	
diff=10	2	0	0	0	0	0	2	
diff=11	4	0	1	1	1	0	7	
diff=12	5	1	0	1	0	0	7	
sum	28	2	2	4	4	0	40	

Chimera analysis: relative delta Hamming distances
	FS=1	FS=2	FS=3	FS=4	FS=5-10	FS>10	sum	
diff=0.0	1	0	0	0	0	0	1	
diff=0.7	5	0	0	0	0	0	5	
diff=0.8	3	0	0	1	1	0	5	
diff=1.0	19	2	2	3	3	0	29	
sum	28	2	2	4	4	0	40	

Chimeras:
All tags were filtered: only those tags where at least one half is identical with the half of the min. tag are kept.
So the Hamming distance of the non-identical half is shown.
Be aware that the real number of chimeric tags (where rel. diff = 1) is not shown in the plot because of the above reasons.
real number of chimeric tags	20	1.0
Hamming distances of non-zero half
	FS=1	FS=2	FS=3	FS=4	FS=5-10	FS>10	sum	
HD=1	4	1	1	1	1	0	8	
HD=7	2	0	0	0	0	0	2	
HD=8	1	0	0	0	1	0	2	
HD=9	1	0	0	0	0	0	1	
HD=10	2	0	0	0	0	0	2	
HD=11	4	0	1	1	1	0	7	
HD=12	5	1	0	1	0	0	7	
sum	19	2	2	3	3	0	29