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comparison test-data/output_file.tabular @ 22:7e570ba56b83 draft

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planemo upload for repository https://github.com/monikaheinzl/duplexanalysis_galaxy/tree/master/tools/hd commit b8a2f7b7615b2bcd3b602027af31f4e677da94f6-dirty
author mheinzl
date Wed, 27 Feb 2019 04:50:56 -0500
parents 2e9f7ea7ae93
children
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21:9919024d7778 22:7e570ba56b83
18 FS=6 1 0 0 0 0 0 1 18 FS=6 1 0 0 0 0 0 1
19 FS=7 0 0 0 0 1 0 1 19 FS=7 0 0 0 0 1 0 1
20 sum 9 0 0 0 11 0 20 20 sum 9 0 0 0 11 0 20
21 21
22 22
23 max. family size: 7 23 max. family size in sample: 7
24 absolute frequency: 1 24 absolute frequency: 1
25 relative frequency: 0.05 25 relative frequency: 0.05
26 26
27 The hamming distances were calculated by comparing each half of all tags against the tag(s) with the minimum Hamming distance per half. 27 The hamming distances were calculated by comparing each half of all tags against the tag(s) with the minimum Hamming distance per half.
28 It is possible that one tag can have the minimum HD from multiple tags, so the sample size in this calculation differs from the sample size entered by the user. 28 Since this calculation was repeated, but starting with the second half to find all possible chimeras in the data, the actual number of tags in the plots differs from the sample size entered by the user.
29 actual number of tags with min HD = 171 (sample size by user = 20) 29 In addition, both family sizes of one tag will be included in the plots if only tags of reads that can form a DCS were allowed.
30 length of one part of the tag = 12 30 length of one part of the tag = 12
31 31
32 Hamming distance of each half in the tag 32 Hamming distance of each half in the tag
33 HD a HD b' HD b HD a' HD a+b sum 33 HD a HD b' HD b HD a' HD a+b sum
34 HD=0 146 0 8 4 0 158 34 HD=0 20 0 8 1 0 29
35 HD=1 0 2 2 21 11 36 35 HD=1 0 0 1 19 8 28
36 HD=2 0 0 0 0 1 1 36 HD=2 0 0 0 0 1 1
37 HD=5 0 0 4 0 0 4 37 HD=5 0 0 3 0 0 3
38 HD=6 0 2 2 0 6 10 38 HD=6 0 0 2 0 3 5
39 HD=7 0 16 9 0 21 46 39 HD=7 0 1 6 0 4 11
40 HD=8 0 20 0 0 26 46 40 HD=8 0 2 0 0 7 9
41 HD=9 0 50 0 0 50 100 41 HD=9 0 1 0 0 1 2
42 HD=10 0 30 0 0 30 60 42 HD=10 0 2 0 0 2 4
43 HD=11 0 18 0 0 18 36 43 HD=11 0 7 0 0 7 14
44 HD=12 0 8 0 0 8 16 44 HD=12 0 7 0 0 7 14
45 sum 146 146 25 25 171 513 45 sum 20 20 20 20 40 120
46 46
47 Absolute delta Hamming distances within the tag 47 Absolute delta Hamming distances within the tag
48 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum 48 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum
49 diff=0 1 0 0 0 0 0 1 49 diff=0 1 0 0 0 0 0 1
50 diff=1 6 1 2 1 1 0 11 50 diff=1 4 1 1 1 1 0 8
51 diff=4 4 0 0 0 0 0 4 51 diff=4 3 0 0 0 0 0 3
52 diff=5 2 0 0 0 0 0 2 52 diff=5 2 0 0 0 0 0 2
53 diff=6 6 0 0 1 1 0 8 53 diff=6 3 0 0 1 1 0 5
54 diff=7 15 0 1 0 3 0 19 54 diff=7 2 0 0 0 0 0 2
55 diff=8 15 2 0 1 2 0 20 55 diff=8 1 0 0 0 1 0 2
56 diff=9 37 4 1 4 4 0 50 56 diff=9 1 0 0 0 0 0 1
57 diff=10 22 2 1 4 1 0 30 57 diff=10 2 0 0 0 0 0 2
58 diff=11 8 1 1 5 3 0 18 58 diff=11 4 0 1 1 1 0 7
59 diff=12 6 1 0 1 0 0 8 59 diff=12 5 1 0 1 0 0 7
60 sum 122 11 6 17 15 0 171 60 sum 28 2 2 4 4 0 40
61 61
62 Chimera analysis: relative delta Hamming distances 62 Chimera analysis: relative delta Hamming distances
63 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum 63 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum
64 diff=0.0 1 0 0 0 0 0 1 64 diff=0.0 1 0 0 0 0 0 1
65 diff=0.7 6 0 0 0 0 0 6 65 diff=0.7 5 0 0 0 0 0 5
66 diff=0.8 4 0 0 1 1 0 6 66 diff=0.8 3 0 0 1 1 0 5
67 diff=1.0 111 11 6 16 14 0 158 67 diff=1.0 19 2 2 3 3 0 29
68 sum 122 11 6 17 15 0 171 68 sum 28 2 2 4 4 0 40
69 69
70 Chimeras: 70 Chimeras:
71 All tags were filtered: only those tags where at least one half is identical with the half of the min. tag are kept. 71 All tags were filtered: only those tags where at least one half is identical with the half of the min. tag are kept.
72 So the hamming distance of the non-identical half is compared. 72 So the Hamming distance of the non-identical half is shown.
73 Be aware that the real number of chimeric tags (where rel. diff = 1) is not shown in the plot because of the above reasons.
74 real number of chimeric tags 20 1.0
73 Hamming distances of non-zero half 75 Hamming distances of non-zero half
74 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum 76 FS=1 FS=2 FS=3 FS=4 FS=5-10 FS>10 sum
75 HD=1 6 1 2 1 1 0 11 77 HD=1 4 1 1 1 1 0 8
76 HD=6 2 0 0 0 0 0 2 78 HD=7 2 0 0 0 0 0 2
77 HD=7 15 0 1 0 3 0 19 79 HD=8 1 0 0 0 1 0 2
78 HD=8 15 2 0 1 2 0 20 80 HD=9 1 0 0 0 0 0 1
79 HD=9 37 4 1 4 4 0 50 81 HD=10 2 0 0 0 0 0 2
80 HD=10 22 2 1 4 1 0 30 82 HD=11 4 0 1 1 1 0 7
81 HD=11 8 1 1 5 3 0 18 83 HD=12 5 1 0 1 0 0 7
82 HD=12 6 1 0 1 0 0 8 84 sum 19 2 2 3 3 0 29
83 sum 111 11 6 16 14 0 158
84 85
85 86