comparison read2mut.xml @ 19:858ca8b7ad43 draft

planemo upload for repository https://github.com/Single-Molecule-Genetics/VariantAnalyzerGalaxy/tree/master/tools/variant_analyzer commit ee4a8e6cf290e6c8a4d55f9cd2839d60ab3b11c8

18:d910b6dfd826

<?xml version="1.0" encoding="UTF-8"?>
<tool id="read2mut" name="Call specific mutations in reads:" version="2.0.3" profile="17.01">
    <description>Looks for reads with mutation at known positions and calculates frequencies and stats.</description>
    <macros>
        <import>va_macros.xml</import>
    </macros>
    <expand macro="requirements">

        --bamFile bam_input.bam
        --inputJson '$file3'
        --sscsJson '$file4'
        --thresh '$thresh'
        --phred '$phred'
        --trim '$trim'
        $chimera_correction
        --outputFile '$output_xlsx'
        --outputFile_csv '$outputFile_csv'
        --outputFile2 '$output_xlsx2'
        --outputFile3 '$output_xlsx3'

        <param name="file2" type="data" format="bam" label="BAM File of raw reads" optional="false" help="BAM file with aligned raw reads of selected tags."/>
        <param name="file3" type="data" format="json" label="JSON File with DCS tag stats" optional="false" help="JSON file generated by DCS mutations to tags/reads"/>
        <param name="file4" type="data" format="json" label="JSON File with SSCS tag stats" optional="false" help="JSON file generated by DCS mutations to SSCS stats."/>
        <param name="thresh" type="integer" label="Tag count threshold" value="0" help="Integer threshold for displaying mutations. Only mutations occuring in DCS of less than thresh tags are displayed. Default of 0 displays all."/>
        <param name="phred" type="integer" label="Phred quality score threshold" min="0" max="41" value="20" help="Integer threshold for Phred quality score. Only reads higher than this threshold are considered. Default = 20."/>
        <param name="trim" type="integer" label="Trimming threshold" value="10" help="Integer threshold for assigning mutations at start and end of reads to lower tier. Default 10."/>
        <param name="chimera_correction" type="boolean" label="Apply chimera correction?" truevalue="--chimera_correction" falsevalue="" checked="False" help="Count chimeric variants and correct the variant frequencies."/>
    </inputs>
    <outputs>
        <data name="output_xlsx" format="xlsx" label="${tool.name} on ${on_string}: XLSX summary"/>
        <data name="outputFile_csv" format="csv" label="${tool.name} on ${on_string}: CSV summary"/>

            <param name="file2" value="Interesting_Reads_test.trim.bam"/>
            <param name="file3" value="tag_count_dict_test.json"/>
            <param name="file4" value="SSCS_counts_test.json"/>
            <param name="thresh" value="0"/>
            <param name="phred" value="20"/>
            <param name="trim" value="10"/>
            <param name="delim_csv" value=","/>
            <output name="output_xlsx" file="Variant_Analyzer_summary_test.xlsx" decompress="true" lines_diff="10"/>
            <output name="outputFile_csv" file="Variant_Analyzer_summary_test.csv" decompress="true" lines_diff="10"/>
            <output name="output_xlsx2" file="Variant_Analyzer_allele_frequencies_test.xlsx" decompress="true" lines_diff="10"/>
            <output name="output_xlsx3" file="Variant_Analyzer_tiers_test.xlsx" decompress="true" lines_diff="10"/>

19:858ca8b7ad43

<?xml version="1.0" encoding="UTF-8"?>
<tool id="read2mut" name="Call specific mutations in reads:" version="2.0.4" profile="17.01">
    <description>Looks for reads with mutation at known positions and calculates frequencies and stats.</description>
    <macros>
        <import>va_macros.xml</import>
    </macros>
    <expand macro="requirements">

        --bamFile bam_input.bam
        --inputJson '$file3'
        --sscsJson '$file4'
        --thresh '$thresh'
        --phred '$phred'
        --trim5 '$trim5'
        --trim3 '$trim3'
        $chimera_correction
        --outputFile '$output_xlsx'
        --outputFile_csv '$outputFile_csv'
        --outputFile2 '$output_xlsx2'
        --outputFile3 '$output_xlsx3'

        <param name="file2" type="data" format="bam" label="BAM File of raw reads" optional="false" help="BAM file with aligned raw reads of selected tags."/>
        <param name="file3" type="data" format="json" label="JSON File with DCS tag stats" optional="false" help="JSON file generated by DCS mutations to tags/reads"/>
        <param name="file4" type="data" format="json" label="JSON File with SSCS tag stats" optional="false" help="JSON file generated by DCS mutations to SSCS stats."/>
        <param name="thresh" type="integer" label="Tag count threshold" value="0" help="Integer threshold for displaying mutations. Only mutations occuring in DCS of less than thresh tags are displayed. Default of 0 displays all."/>
        <param name="phred" type="integer" label="Phred quality score threshold" min="0" max="41" value="20" help="Integer threshold for Phred quality score. Only reads higher than this threshold are considered. Default = 20."/>
        <param name="trim5" type="integer" label="Trimming threshold at 5' end" value="10" help="Integer threshold for assigning mutations at start of reads to lower tier. Default 10."/>
        <param name="trim3" type="integer" label="Trimming threshold at 3' end" value="10" help="Integer threshold for assigning mutations at end of reads to lower tier. Default 10."/>
        <param name="chimera_correction" type="boolean" label="Apply chimera correction?" truevalue="--chimera_correction" falsevalue="" checked="False" help="Count chimeric variants and correct the variant frequencies."/>
    </inputs>
    <outputs>
        <data name="output_xlsx" format="xlsx" label="${tool.name} on ${on_string}: XLSX summary"/>
        <data name="outputFile_csv" format="csv" label="${tool.name} on ${on_string}: CSV summary"/>

            <param name="file2" value="Interesting_Reads_test.trim.bam"/>
            <param name="file3" value="tag_count_dict_test.json"/>
            <param name="file4" value="SSCS_counts_test.json"/>
            <param name="thresh" value="0"/>
            <param name="phred" value="20"/>
            <param name="trim5" value="10"/>
            <param name="trim3" value="10"/>
            <param name="delim_csv" value=","/>
            <output name="output_xlsx" file="Variant_Analyzer_summary_test.xlsx" decompress="true" lines_diff="10"/>
            <output name="outputFile_csv" file="Variant_Analyzer_summary_test.csv" decompress="true" lines_diff="10"/>
            <output name="output_xlsx2" file="Variant_Analyzer_allele_frequencies_test.xlsx" decompress="true" lines_diff="10"/>
            <output name="output_xlsx3" file="Variant_Analyzer_tiers_test.xlsx" decompress="true" lines_diff="10"/>