Mercurial > repos > mingchen0919 > aurora_fastqc_site
annotate 04_per_sequence_quality_score.Rmd @ 2:c64267b9f754 draft default tip
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author | mingchen0919 |
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date | Sun, 30 Dec 2018 12:48:14 -0500 |
parents | b7c115edd970 |
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0 | 1 --- |
2 output: html_document | |
3 --- | |
4 | |
5 ```{r setup, include=FALSE, warning=FALSE, message=FALSE} | |
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6 knitr::opts_knit$set(progress = FALSE) |
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7 knitr::opts_chunk$set(error = TRUE, echo = FALSE) |
0 | 8 ``` |
9 | |
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10 ### Per sequence quality score |
0 | 11 |
12 ```{r 'Per sequence quality score', fig.width=10} | |
13 ## reads 1 | |
14 psqs_1 = extract_data_module(paste0(opt$X_d, '/read_1_fastqc/fastqc_data.txt'), 'Per sequence quality scores') | |
15 psqs_1$trim = 'before' | |
16 | |
17 ## reads 2 | |
18 psqs_2 = extract_data_module(paste0(opt$X_d, '/read_2_fastqc/fastqc_data.txt'), 'Per sequence quality scores') | |
19 psqs_2$trim = 'after' | |
20 | |
21 comb_psqs = rbind(psqs_1, psqs_2) | |
22 comb_psqs$trim = factor(levels = c('before', 'after'), comb_psqs$trim) | |
23 | |
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24 p = ggplot(data = comb_psqs) + |
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25 geom_line(mapping = aes(x = X.Quality, y = Count), color = 'red') + |
0 | 26 facet_grid(. ~ trim) + |
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27 scale_x_continuous(name = 'Mean Sequence Qaulity (Phred Score)', |
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28 limits = c(min(comb_psqs$X.Quality), max(comb_psqs$X.Quality))) + |
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29 scale_y_continuous(name = '') + |
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30 theme(panel.background = element_rect(fill = NA), |
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31 axis.line = element_line(), |
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32 panel.grid.major.y = element_line(color = 'blue', size = 0.1)) |
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33 p |
0 | 34 ``` |
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35 |