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1 <tool id="DESeq_visualization" name="DESeq2: Visualization" version="1.0.0">
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2 <requirements>
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3 <requirement type="package" version="1.14.1">bioconductor-deseq2</requirement>
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4 <requirement type="package" version="1.20.0">r-getopt</requirement>
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5 <requirement type="package" version="1.2">r-rmarkdown</requirement>
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6 <requirement type="package" version="1.8.4">r-plyr</requirement>
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7 <requirement type="package" version="1.1.0">r-stringr</requirement>
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8 <requirement type="package" version="0.4.0">r-highcharter</requirement>
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9 <requirement type="package" version="0.2">r-dt</requirement>
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10 <requirement type="package" version="1.4.2">r-reshape2</requirement>
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11 <requirement type="package" version="4.5.6">r-plotly</requirement>
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12 <requirement type="package" version="0.2.0.1">r-formattable</requirement>
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13 <requirement type="package" version="0.3.5">r-htmltools</requirement>
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13
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14 <requirement type="package" version="1.0.8">r-pheatmap</requirement>
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15 </requirements>
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16 <description>
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17 An R Markdown tool to visualize DESeq analysis results.
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18 </description>
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19 <stdio>
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20 <regex match="Execution halted"
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21 source="both"
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22 level="fatal"
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23 description="Execution halted." />
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24 <regex match="Error in"
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25 source="both"
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26 level="fatal"
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27 description="An undefined error occured, please check your intput carefully and contact your administrator." />
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28 <regex match="Fatal error"
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29 source="both"
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30 level="fatal"
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31 description="An undefined error occured, please check your intput carefully and contact your administrator." />
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32 </stdio>
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33 <command>
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34 <![CDATA[
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35
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36 Rscript '${__tool_directory__}/DESeq_visualization_render.R'
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37
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38 ## 1. input data
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39 -e $echo
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40 -w $deseq_workspace
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41
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42 #set $pca_groups = []
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43 #for $group in $intgroups_pca
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44 #if str($group.intgroup)
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45 #set $pca_groups = $pca_groups + [str($group.intgroup)]
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46 #end if
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47 #end for
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48 #set $pca_groups = ','.join($pca_groups)
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49 -p "$pca_groups"
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50
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51 #set $mds_groups = []
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52 #for $group in $intgroups_mds
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53 #if str($group.intgroup)
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54 #set $mds_groups = $mds_groups + [str($group.intgroup)]
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55 #end if
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56 #end for
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57 #set $mds_groups = ','.join($mds_groups)
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58 -m "$mds_groups"
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59
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60 ## 2. output report and report site directory
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61 -o $deseq_visualization
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62 -d $deseq_visualization.files_path
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63
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64
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65 ## 3. Rmd templates sitting in the tool directory
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66
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67 ## _site.yml and index.Rmd template files
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68 -D '${__tool_directory__}/DESeq_visualization.Rmd'
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69
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70
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71
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72 ]]>
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73 </command>
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74 <inputs>
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75 <param type="data" name="deseq_workspace" format="rdata" multiple="false" label="Workspace from tool DESeq2: DESeq" />
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76 <param type="data" name="sample_table" format="tabular" multiple="false" label="Sample table file" />
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77 <repeat name="intgroups_pca" title="Interest groups for PCA plot" min="1">
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78 <param type="data_column" name="intgroup" data_ref="sample_table" use_header_names="true"
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79 optional="false"
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80 label="Interest group for PCA plot"
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81 help=""/>
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82 </repeat>
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83 <repeat name="intgroups_mds" title="Interest groups for MDS plot" min="1">
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84 <param type="data_column" name="intgroup" data_ref="sample_table" use_header_names="true"
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85 optional="false"
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86 label="Interest group for MDS plot"
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87 help=""/>
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88 </repeat>
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89 <param type="boolean" name="echo" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Display analysis code in report?" />
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90 </inputs>
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91 <outputs>
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92 <data name="deseq_visualization" format="html" label="DESeq Visualization" />
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93 </outputs>
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94 </tool> |
