view DESeq_results.xml @ 3:ab57855151b1 draft

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<tool id="DESeq_results" name="DESeq2: Results" version="1.0.0">
    <requirements>
        <requirement type="package" version="1.14.1">bioconductor-deseq2</requirement>
        <requirement type="package" version="1.20.0">r-getopt</requirement>
        <requirement type="package" version="1.2">r-rmarkdown</requirement>
        <requirement type="package" version="1.8.4">r-plyr</requirement>
        <requirement type="package" version="1.1.0">r-stringr</requirement>
        <requirement type="package" version="0.4.0">r-highcharter</requirement>
        <requirement type="package" version="0.2">r-dt</requirement>
        <requirement type="package" version="1.4.2">r-reshape2</requirement>
        <requirement type="package" version="4.5.6">r-plotly</requirement>
        <requirement type="package" version="0.2.0.1">r-formattable</requirement>
        <requirement type="package" version="0.3.5">r-htmltools</requirement>
    </requirements>
    <description>
        An R Markdown tool to display DESeq analysis.
    </description>
    <stdio>
        <regex match="Execution halted"
               source="both"
               level="fatal"
               description="Execution halted." />
        <regex match="Error in"
               source="both"
               level="fatal"
               description="An undefined error occured, please check your intput carefully and contact your administrator." />
        <regex match="Fatal error"
               source="both"
               level="fatal"
               description="An undefined error occured, please check your intput carefully and contact your administrator." />
    </stdio>
    <command>
        <![CDATA[
        ## Add tools to PATH
        export PATH=/opt/R-3.2.5/bin:\$PATH &&

        Rscript '${__tool_directory__}/DESeq_results_render.R'

            ## 1. input data
            -e $echo
            -w $deseq_workspace
            -c "$contrast_group"
            -t $treatment
            -k $condition

            #set $groups = []
            #for $c_group in $clustering_groups
                #if str($c_group.group)
                    #set $groups = $groups + [str($c_group.group)]
                #end if
            #end for
            #set $groups = ','.join($groups)
            -m "$groups"

            ## 2. output report and report site directory
		    -o $deseq_results
		    -d $deseq_results.files_path

		    ## 3. Rmd templates sitting in the tool directory

		        ## _site.yml and index.Rmd template files
                -D '${__tool_directory__}/DESeq_results.Rmd'



        ]]>
    </command>
    <inputs>
        <param type="data" name="deseq_workspace" format="rdata" multiple="false" label="Workspace from tool DESeq2: DESeq" />
        <param type="data" name="sample_table" format="tabular" multiple="false" label="Sample table file" />
        <param type="data_column" name="contrast_group" data_ref="sample_table" use_header_names="true"
               optional="false"
               label="Group for result contrast"
               help=""/>
        <param type="text" name="treatment" label="Treatment level" />
        <param type="text" name="condition" label="Condition level" />

        <repeat name="clustering_groups" title="Gene clustering groups" min="1">
            <param type="data_column" name="group" data_ref="sample_table" use_header_names="true"
                   optional="false"
                   label="A phenotype column from the sample table" />
        </repeat>

        <param type="boolean" name="echo" truevalue="TRUE" falsevalue="FALSE" checked="false" label="Display analysis code in report?" />
    </inputs>
    <outputs>
        <data name="deseq_results" format="html" label="DESeq Results" />
    </outputs>
</tool>