Mercurial > repos > mingchen0919 > rmarkdown_fastqc_site
comparison 08_sequence_length_distribution.Rmd @ 11:507eec497730 draft
update fastqc site
author | mingchen0919 |
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date | Tue, 07 Nov 2017 16:52:24 -0500 |
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1 --- | |
2 title: 'Sequence Length Distribution' | |
3 output: | |
4 html_document: | |
5 number_sections: true | |
6 toc: true | |
7 theme: cosmo | |
8 highlight: tango | |
9 --- | |
10 | |
11 ```{r setup, include=FALSE, warning=FALSE, message=FALSE} | |
12 knitr::opts_chunk$set( | |
13 echo = ECHO, | |
14 error = TRUE | |
15 ) | |
16 ``` | |
17 | |
18 ### Sequence Length Distribution | |
19 | |
20 ```{r 'Sequence Length Distribution', fig.width=10} | |
21 ## reads 1 | |
22 sld_1 = extract_data_module('REPORT_DIR/reads_1_fastqc_data.txt', 'Sequence Length Distribution') | |
23 sld_1$id = 1:length(sld_1$X.Length) | |
24 sld_1$trim = 'before' | |
25 | |
26 ## reads 2 | |
27 sld_2 = extract_data_module('REPORT_DIR/reads_2_fastqc_data.txt', 'Sequence Length Distribution') | |
28 sld_2$id = 1:length(sld_2$X.Length) | |
29 sld_2$trim = 'after' | |
30 | |
31 comb_sld = rbind(sld_1, sld_2) | |
32 comb_sld$trim = factor(levels = c('before', 'after'), comb_sld$trim) | |
33 | |
34 p = ggplot(data = comb_sld, aes(x = id, y = Count)) + | |
35 geom_line(color = 'red') + | |
36 scale_x_continuous(breaks = sld_2$id, labels = sld_2$X.Length) + | |
37 facet_grid(. ~ trim) + | |
38 xlab('Sequence Length (bp)') + | |
39 ylab('') + | |
40 theme(axis.text.x = element_text(angle=45)) | |
41 ggplotly(p) | |
42 ``` |