comparison 08_sequence_length_distribution.Rmd @ 11:507eec497730 draft

update fastqc site
author mingchen0919
date Tue, 07 Nov 2017 16:52:24 -0500
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10:600c39b11913 11:507eec497730
1 ---
2 title: 'Sequence Length Distribution'
3 output:
4 html_document:
5 number_sections: true
6 toc: true
7 theme: cosmo
8 highlight: tango
9 ---
10
11 ```{r setup, include=FALSE, warning=FALSE, message=FALSE}
12 knitr::opts_chunk$set(
13 echo = ECHO,
14 error = TRUE
15 )
16 ```
17
18 ### Sequence Length Distribution
19
20 ```{r 'Sequence Length Distribution', fig.width=10}
21 ## reads 1
22 sld_1 = extract_data_module('REPORT_DIR/reads_1_fastqc_data.txt', 'Sequence Length Distribution')
23 sld_1$id = 1:length(sld_1$X.Length)
24 sld_1$trim = 'before'
25
26 ## reads 2
27 sld_2 = extract_data_module('REPORT_DIR/reads_2_fastqc_data.txt', 'Sequence Length Distribution')
28 sld_2$id = 1:length(sld_2$X.Length)
29 sld_2$trim = 'after'
30
31 comb_sld = rbind(sld_1, sld_2)
32 comb_sld$trim = factor(levels = c('before', 'after'), comb_sld$trim)
33
34 p = ggplot(data = comb_sld, aes(x = id, y = Count)) +
35 geom_line(color = 'red') +
36 scale_x_continuous(breaks = sld_2$id, labels = sld_2$X.Length) +
37 facet_grid(. ~ trim) +
38 xlab('Sequence Length (bp)') +
39 ylab('') +
40 theme(axis.text.x = element_text(angle=45))
41 ggplotly(p)
42 ```