changeset 1:b434ba108e9b draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/soft-threshold.R	Mon Feb 27 22:36:54 2017 -0500
@@ -0,0 +1,98 @@
+#!/usr/binenv Rscript
+
+# A command-line interface to WGCNA for use with galaxy
+
+
+# setup R error handline to go to stderr
+options(show.error.messages=FALSE,
+        error=function(){
+          cat(geterrmessage(), file=stderr())
+          quit("no", 1, F)
+        })
+
+# we need that to not crash galaxy with an UTF8 error on German LC settings.
+loc = Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
+
+# suppress warning
+options(warn = -1)
+
+suppressPackageStartupMessages({
+  library(getopt)
+  library(tools)
+})
+
+options(stringsAsFactors=FALSE, useFancyQuotes=FALSE)
+args = commandArgs(trailingOnly=TRUE)
+
+# column 1: the long flag name
+# column 2: the short flag alias. A SINGLE character string
+# column 3: argument mask
+#           0: no argument
+#           1: argument required
+#           2: argument is optional
+# column 4: date type to which the flag's argument shall be cast.
+#           possible values: logical, integer, double, complex, character.
+spec_list=list()
+spec_list$help = c('help', 'h', '0', 'logical')
+spec_list$threads = c('threads', '-t', '2', 'integer')
+spec_list$expressionData = c('expressionData', 'f', '1', 'character')
+spec_list$betaMaximum = c('betaMaximum', 'b', 1, 'integer')
+spec_list$rPowerTableOutput = c('rPowerTableOutput', 'r', 1, 'character')
+spec_list$scaleFreeFitPlot = c('scaleFreeFitPlot', 'p', 1, 'character')
+spec = t(as.data.frame(spec_list))
+
+opt = getopt(spec)
+# arguments are accessed by long flag name (the first column in the spec matrix)
+#                        NOT by element name in the spec_list
+# example: opt$help, opt$expression_file
+
+suppressPackageStartupMessages({
+  library(MASS)
+  library(ggplot2)
+  library(ggdendro)
+  library(class)
+  library(cluster)
+  library(impute)
+  library(Hmisc)
+  library(WGCNA)
+})
+
+# allow multi-threads for WGCNA
+if(!is.null(opt$threads)){
+  allowWGCNAThreads(nThreads = opt$threads)
+}
+# disableWGCNAThreads()
+
+# read expression data
+#   column names are genes, rows are samples
+expressionData = read.csv(opt$expressionData, header = TRUE, row.names = 1)
+
+cat("Calculate R power table\n")
+powers = seq(1, opt$betaMaximum)
+RpowerTable = pickSoftThreshold(expressionData, powerVector = powers)[[2]]
+cat("write R power table into file\n")
+write.csv(RpowerTable, file=opt$rPowerTableOutput)
+
+
+pdf(file = opt$scaleFreeFitPlot)
+
+Rpower = RpowerTable[,1]
+# plot scale free fit R^2 versus different soft threshold beta
+signedRSq = -sign(RpowerTable[, 3]) * RpowerTable[, 2]
+Rpower_df = data.frame(Rpower, signedRSq)
+
+p = ggplot(aes(x = Rpower, y = signedRSq), data = Rpower_df)
+p + geom_label(label = Rpower, color = "red") +
+  xlab("R power") +
+  ylab(expression("Scale Free Topology Model Fit, Signed "~R^2)) +
+  geom_hline(yintercept = 0.95, color = "blue")
+
+# mean connectivity versus different soft threshold beta
+meanConn = RpowerTable[,5]
+meanConn_df = data.frame(Rpower, meanConn)
+p = ggplot(aes(x = Rpower, y = meanConn), data = meanConn_df)
+p + geom_label(label = Rpower, color = "red") +
+  xlab("R power") +
+  ylab("Mean connectivity")
+
+dev.off()
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