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1 <tool id="mmseq" name="MMSEQ">
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2 <description>Estimation of transcript expression</description>
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3 <command interpreter="bash">mmseq.sh $input1 $input2 $output1 $output2 $output3</command>
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4 <inputs>
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5 <param format="fasta" name="input1" type="data" label="Reference genome"/>
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6 <param format="bam" name="input2" type="data" label="Sorted bam file"/>
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7 </inputs>
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8 <outputs>
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9 <data format="tabular" name="output1"/>
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10 <data format="tabular" name="output2"/>
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11 <data format="tabular" name="output3"/>
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12 </outputs>
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13 <help>
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14
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15 **About MMSEQ**
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16
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17 MMSEQ_ is a novel RNA seq analysis method for estimating haplotype, isoform and gene specific expression.
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18
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19 .. _MMSEQ: http://www.bgx.org.uk/software/mmseq.html
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20
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21 --------
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22
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23 **Input formats**::
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24
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25 MMSEQ accepts sorted BAM file as input. The SAM files obtained as a result of Bowtie alignment can be converted to BAM files and sorted using the SAMTools.
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26
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27 --------
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28
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29 **Outputs**::
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30
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31 </help>
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32 </tool>
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