# HG changeset patch # User modencode-dcc # Date 1358537828 18000 # Node ID e207763f91cbca834248baaa8eb3f62ee9d93d64 # Parent 390a709f0eca415553345d871f7873e657a10713 Deleted selected files diff -r 390a709f0eca -r e207763f91cb ranger_wrapper.xml --- a/ranger_wrapper.xml Fri Jan 18 14:15:20 2013 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,254 +0,0 @@ - - multi-purpose, ultrafast ChIP Seq peak caller - ranger_wrapper.py $options_file $outputs_file - - peakranger - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - major_command['major_command_selector'] == 'nr' or major_command['major_command_selector'] == 'lc' or major_command['major_command_selector'] == 'ccat' - - - major_command['major_command_selector'] == 'wig' or major_command['major_command_selector'] == 'wigpe' - - - major_command['major_command_selector'] == 'ranger' or major_command['major_command_selector'] == 'ccat' - - - major_command['major_command_selector'] == 'ranger' or major_command['major_command_selector'] == 'ccat' - - - major_command['major_command_selector'] == 'ranger' or major_command['major_command_selector'] == 'ccat' - - - major_command['major_command_selector'] == 'ranger' or major_command['major_command_selector'] == 'ccat' - major_command['gene_annotate_file']['gene_annotate_file_selector'] != 'None' - - - - - <% -import simplejson -%> -##======================================================================================= -#set $__outputs = { 'command':str( $major_command.major_command_selector ) } -#if str( $major_command.major_command_selector ) == 'nr' or str( $major_command.major_command_selector ) == 'lc': - #set $__outputs['output_ranger_file'] = str( $output_ranger_file ) -#end if -##======================================================================================= -#if str( $major_command.major_command_selector ) == 'wig' or str( $major_command.major_command_selector ) == 'wigpe': - #set $__outputs['output_wigzip_file'] = str( $output_wigzip_file ) -#end if -##======================================================================================= -#if str( $major_command.major_command_selector ) == 'ranger' or str( $major_command.major_command_selector ) == 'ccat': - #set $__outputs['output_summit_file'] = str( $output_summit_file ) - #set $__outputs['output_region_file'] = str( $output_region_file ) - #set $__outputs['output_details_file'] = str( $output_details_file ) - #set $__outputs['output_report_file'] = str( $output_report_file ) - #set $__outputs['output_ranger_file'] = str( $output_ranger_file ) -#end if -##======================================================================================= -${ simplejson.dumps( __outputs ) } - - <% -import simplejson -%> -#set $__options = { 'experiment_name':str($experiment_name), 'action':str( $major_command.major_command_selector ) } - -##============================================================================= -#if str($major_command.major_command_selector) == 'nr': - #set $__options['chip_file'] = str( $major_command.input_chipseq_file1 ) - #set $__options['input_file'] = str( $major_command.input_control_file1 ) - - #set $__options['extension'] = str( $major_command.extension ) -#end if -##============================================================================= -##if str($major_command.major_command_selector) == 'lc': - #set $__options['chip_file'] = str( $major_command.input_chipseq_file1 ) -##end if -##============================================================================= -#if str($major_command.major_command_selector) == 'wig' or str($major_command.major_command_selector) == 'wigpe': - #set $__options['chip_file'] = str( $major_command.input_chipseq_file1 ) - - #set $__options['extension'] = str( $major_command.extension ) - #set $__options['split'] = str( $major_command.split ) - #set $__options['strand'] = str( $major_command.strand) - #set $__options['gzip'] = str( $major_command.gzip ) -#end if -##============================================================================= -#if str($major_command.major_command_selector) == 'ranger': - #set $__options['chip_file'] = str( $major_command.input_chipseq_file1 ) - #set $__options['input_file'] = str( $major_command.input_control_file1 ) - - #set $__options['gene_annotate_file'] = str( $major_command.gene_annotate_file.gene_annotate_file_selector ) - #if str( $major_command.gene_annotate_file.gene_annotate_file_selector ) == 'Upload' - #set $__options['usr_annot_file'] = str( $major_command.gene_annotate_file.usr_annot_file ) - #end if - - #set $__options['plot_region'] = str( $major_command.plot_region ) - #set $__options['pvalue'] = str( $major_command.pvalue ) - #set $__options['fdr'] = str( $major_command.fdr ) - #set $__options['extension'] = str( $major_command.extension ) - #set $__options['delta'] = str( $major_command.delta ) - #set $__options['threads'] = str( $major_command.threads ) - #set $__options['bandwith'] = str( $major_command.bandwith ) - #set $__options['pad'] = str( $major_command.pad ) -#end if -##============================================================================= -#if str($major_command.major_command_selector) == 'ccat': - #set $__options['chip_file'] = str( $major_command.input_chipseq_file1 ) - #set $__options['input_file'] = str( $major_command.input_control_file1 ) - - #set $__options['gene_annotate_file'] = str( $major_command.gene_annotate_file.gene_annotate_file_selector ) - #if str( $major_command.gene_annotate_file.gene_annotate_file_selector ) == 'Upload' - #set $__options['usr_annot_file'] = str( $major_command.gene_annotate_file.usr_annot_file ) - #end if - - #set $__options['plot_region'] = str( $major_command.plot_region ) - #set $__options['fdr'] = str( $major_command.fdr ) - #set $__options['extension'] = str( $major_command.extension ) - #set $__options['winsize'] = str( $major_command.winsize ) - #set $__options['winstep'] = str( $major_command.winstep ) - #set $__options['mincount'] = str( $major_command.mincount ) - #set $__options['minscore'] = str( $major_command.minscore ) -#end if -##============================================================================= -${ simplejson.dumps( __options ) } - - - - - - -**What it does** - -PeakRanger is a multi-purporse software suite for analyzing next-generation sequencing (NGS) data. The suite contains the following tools: - -View the modified PeakRanger documentation: http://ranger.sourceforge.net/manualv116.html#introduction - ------- - -**Usage** - -**nr:** noise rate estimator. Estimates signal to noise ratio which is an indicator for ChIP enrichment - -**lc:** library complexity calculator. Calculates the ratio of unique reads over total reads. Only accepts bam files. - -**wig:** coverage file generator. Generates variable step format wiggle file - -**wigpe:** coverage file generator. Generates bedGraph format wiggle file and supports spliced alignments and thus only supports bam files - - wigpe can also generate coverage files for bam files containing spliced reads, such as those from RNA-Seq experiments. - -**ranger:** ChIP-Seq peak caller. It is able to identify enriched genomic regions while at the same time discover summits within these regions. - -**ccat:** ChIP-Seq peak caller. Tuned for the discovery of broad peaks - - Both ranger and ccat supports generating HTML-based annotation reports. - ------- - -**Citation** - -If you use PeakRanger in your research, please cite: -Feng X, Grossman R, Stein L: PeakRanger:A cloud-enabled peak caller for ChIP-seq data.BMC Bioinformatics 2011, 12(1):139. - -if you use the ccat tool, please also cite: -Xu, H., L. Handoko, et al. (2010).A signal-noise model for significance analysis of ChIP-seq with negative control.Bioinformatics 26(9): 1199-1204. - - -Integration of PeakRanger with Galaxy performed by Ziru Zhou ( ziruzhou@gmail.com ). Please send your comments/questions to help@modencode.org. - -