view trtr.xml @ 19:ee55e3a4b53c

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<tool id="trtr" name="TRTR">
	<requirements>
		    <requirement type="package" version="1.0">trtr</requirement>
	</requirements>

	<description>Trim Reads of Tandem Repeat in a fastq file. </description>
	
	<command>
		trtr $input $max_repeat $aggressive > $output
	</command>
	
	<inputs>
		<param format="fastq" name="input" type="data" label="Source file"/>
		<param name="max_repeat" type="integer" value="10" label="Maximum repeat length" />
		<param name="aggressive" type="integer" value="1" label="Aggressive? See description."/>
	</inputs>
	
	<outputs>
		<data format="fastq" name="output" />
	</outputs>

  <tests>
    <test>
      <param name="input" value="small.fastq"/>
      <output name="output" file="smallTrimmed.fastq"/>
    </test>
    <test>
      <param name="input" value="medium.fastq"/>
      <output name="output" file="mediumTrimmed.fastq"/>
    </test>
  </tests>

  <help>
This tool removes tandem repeats from ends of unaligned sequencing reads (leaving one copy). This prevents reads that don't span the repeated region from overlapping and leading to innaccurate SNPs calls.

The maximimum repeat length is adjustable (use 1 to trim only homopolymers).

The "aggressive" option should not be touched in general. Setting to 0 will prevent the program from trimming to exactly 1 copy of the repeat, instead leaving between 1 and 2 copies.

This could also be a useful first step before assembly. More testing needs to be done.
  </help>

</tool>