view goseq.xml @ 13:ea84562a5115 draft

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<tool id="goseq" name="goseq gene ontology analyser" version="0.1.1">
    <description />
    <requirements>
        <requirement type="package" version="3.2.1">R</requirement>
        <requirement type="package" version="1.22.0">goseq</requirement>
    </requirements>
    <command interpreter="Rscript">
        goseq.r --dge_file "$dge_file"
        --p_adj_column "$p_adj_column"
        --cutoff "$p_adj_cutoff"
        --genome "$genome"
        --gene_id "$gene_id"
        --wallenius_tab "$wallenius_tab"
        --sampling_tab "$sampling_tab"
        --nobias_tab "$nobias_tab"
        --length_bias_plot "$length_bias_plot"
        --sample_vs_wallenius_plot "$sample_vs_wallenius_plot"
        --repcnt "$repcnt"
    </command>
    <inputs>
        <param help="deseq2/edger/limma differential gene expression list" label="DGE list" name="dge_file" type="data" format="tabular" />
        <param help="Select the column that contains the multiple-testing corrected p-value" label="p adjust column" name="p_adj_column" type="data_column" data_ref="dge_file"/>
        <param help="Typically 0.05 after multiple testing correction" max="1" label="Minimum p adjust value to consider gene differentially expressed" name="p_adj_cutoff" type="float" value="0.05" />
        <param help="Needed to retrive gene length for length correction" label="Select the genome source" name="genome" size="3" type="select">
            <options from_file="genomes.loc">
                <column name="value" index="0"/>
                <column name="name" index="1"/>
            </options>
        </param>
        <param help="Needed for GO analysis" label="Select gene identifier" name="gene_id" type="select">
            <options from_file="gene_ids.loc">
                <column name="value" index="0"/>
                <column name="name" index="1"/>
            </options>
        </param>
        <param help="Do this many random samplings. Larger values take a long time" label="Number of random sampling" name="repcnt" size="3" type="integer" min="100" max="10000" value="1000" />
    </inputs>
    <outputs>
        <data format="tabular" label="Ranked category list - wallenius" name="wallenius_tab" />
        <data format="tabular" label="Ranked category list - sampling" name="sampling_tab" />
        <data format="tabular" label="Ranked category list - no length bias correction" name="nobias_tab" />
        <data format="pdf" label="length bias plot" name="length_bias_plot" />
        <data format="pdf" label="Plot P-value from sampling against wallenius distribution" name="sample_vs_wallenius_plot" />
    </outputs>
    <tests>
        <test>

        </test>
    </tests>
    <help>

        **What it does**

        Detects Gene Ontology and/or other user defined categories which are over/under represented in RNA-seq data.

        Options map closely to the excellent manual_

        .. _manual: https://bioconductor.org/packages/release/bioc/vignettes/goseq/inst/doc/goseq.pdf


        </help>
    <citations>
            <citation type="doi">10.1186/gb-2010-11-2-r14</citation>
    </citations>
</tool>