# HG changeset patch
# User nate
# Date 1441138538 14400
# Node ID d0c258caafaf7adf0e45ceb214e0b0d620b44c54

Uploaded

diff -r 000000000000 -r d0c258caafaf trinityrnaseq.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/trinityrnaseq.xml	Tue Sep 01 16:15:38 2015 -0400
@@ -0,0 +1,120 @@
+<tool id="trinity_psc" name="Trinity" version="0.0.1">
+
+    <!-- Written by Jeremy Goecks, now maintained here by bhaas and additional
+         modifications by Nate Coraor -->
+    <description>(Beta) De novo assembly of RNA-Seq data Using Trinity on PSC's Greenfield</description>
+    <requirements>
+        <!-- These are versions available as modules on Greenfield -->
+        <requirement type="package" version="1.1.1">bowtie</requirement>
+        <requirement type="package" version="1.1">samtools</requirement>
+        <requirement type="package" version="jre7">java</requirement>
+        <requirement type="package" version="2.0.6">trinity</requirement>
+    </requirements>
+    <command>
+        MEM=`expr "\${GALAXY_SLOTS:-15}" \* 50 - 16` ;
+        Trinity --max_memory "\${MEM}G"
+                --CPU "\${GALAXY_SLOTS:-16}"
+                --bflyHeapSpaceMax "\${MEM}G"
+        
+        ## Inputs.
+        #if str($inputs.paired_or_single) == "paired":
+            --left $inputs.left_input --right $inputs.right_input
+            #if  $inputs.left_input.ext == 'fa':
+                --seqType fa
+            #else:
+                --seqType fq
+            #end if
+            #if str($inputs.library_type) != "undefined":
+                --SS_lib_type $inputs.library_type
+            #end if
+            --group_pairs_distance $inputs.group_pairs_distance
+        #else:
+            --single $inputs.input
+            #if  str($inputs.input.ext) == 'fa':
+                --seqType fa
+            #else:
+                --seqType fq
+            #end if
+            #if str($inputs.library_type) != "undefined":
+                --SS_lib_type $inputs.library_type
+            #end if
+        #end if
+
+        ## Additional parameters.
+        #if str($additional_params.use_additional) == "yes":
+            --min_kmer_cov $additional_params.min_kmer_cov --max_reads_per_graph $additional_params.max_reads_per_graph
+            #if $additional_params.bfly_opts != 'None':
+                --bfly_opts " $additional_params.bfly_opts "
+            #end if
+        #end if
+
+        ## direct to output
+        > $trinity_log 2>&amp;1
+ 
+        ## if Trinity fails, output the end of the log to stderr for Galaxy, and touch the output file for Pulsar
+        || (ec=\$? ; cat $trinity_log >&amp;2 ; mkdir -p trinity_out_dir ; touch trinity_out_dir/Trinity.fasta ; exit \$ec)
+
+    </command>
+    <stdio>
+        <exit_code range="1:" level="fatal" description="Program failed" />
+        <exit_code range=":-1" level="fatal" description="DRM killed job" />
+    </stdio>
+    <inputs>
+        <conditional name="inputs">
+            <param name="paired_or_single" type="select" label="Paired or Single-end data?">
+                <option value="paired">Paired</option>
+                <option value="single">Single</option>
+            </param>
+            <when value="paired">
+                <param format="fasta,fastq" name="left_input" type="data" label="Left/Forward strand reads" help=""/>
+                <param format="fasta,fastq" name="right_input" type="data" label="Right/Reverse strand reads" help=""/>
+                <param name="library_type" type="select" label="Strand-specific Library Type">
+                    <option value="undefined">Not set</option>
+                    <option value="FR">FR</option>
+                    <option value="RF">RF</option>
+                </param>
+                <param name="group_pairs_distance" type="integer" value="500" min="1" label="Group pairs distance" help="Maximum length expected between fragment pairs"/>
+                <param name="path_reinforcement_distance" type="integer" value="75" min="1" label="Path reinforcement distance" help="Minimum read overlap required for path extension in the graph" />    
+            </when>
+            <when value="single">
+                <param format="fasta,fastq" name="input" type="data" label="Single-end reads" help=""/>
+                <param name="library_type" type="select" label="Strand-specific Library Type">
+                    <option value="undefined">Not set</option>
+                    <option value="F">F</option>
+                    <option value="R">R</option>
+                </param>
+                <param name="path_reinforcement_distance" type="integer" value="40" min="1" label="Path reinforcement distance" help="Minimum read overlap required for path extension in the graph" />    
+            </when>
+        </conditional>
+        
+        <conditional name="additional_params">
+            <param name="use_additional" type="select" label="Use Additional Params?">
+                <option value="no">No</option>
+                <option value="yes">Yes</option>
+            </param>
+            <when value="no">
+            </when>
+            <when value="yes">            
+                <param name="min_kmer_cov" type="integer" value="1" min="1" label="inchworm_min_kmer_cov" help="Minimum kmer coverage required by Inchworm for initial contig construction" />
+                <param name="max_reads_per_graph" type="integer" value="20000000" min="10000" label="chrysalis_max_reads_per_graph" help="Maximum number of reads to be anchored within each transcript graph by Chrysalis" />
+                <param name="bfly_opts" type="text" value="None" label="bfly_opts" help="Options to pass on to Butterfly" />
+                <param name="min_contig_length" type="integer" value="200" min="1" label="Minimum Contig Length" help=""/>
+            </when>
+        </conditional>
+    </inputs>
+    <outputs>
+        <data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" />
+        <data format="fasta" name="assembled_transcripts" label="${tool.name} on ${on_string}: Assembled Transcripts" from_work_dir="trinity_out_dir/Trinity.fasta"/>
+    </outputs>
+    <tests>
+    </tests>
+    <help>
+.. warning:: This version of Trinity, which runs on Greenfield_ at the `Pittsburgh Supercomputing Center`_, is a **beta** version. It may not be possible to rerun the same version of this tool, Trinity itself, or its other dependencies (Bowtie, SAMtools) in the future. **When rerunning this tool on the same data, it should, but cannot be guaranteed to reproduce the exact same results to the level of certainty as other Galaxy tools.**
+
+Trinity is a de novo transcript assembler that uses RNA-seq data as input. This tool runs all Trinity_ commands--Inchworm, Chrysalis, and Butterfly--in a single pass.
+        
+.. _Trinity: http://trinityrnaseq.github.io
+.. _Pittsburgh Supercomputing Center: http://www.psc.edu
+.. _Greenfield: http://www.psc.edu/index.php/resources-for-users/computing-resources/greenfield
+    </help>
+</tool>