diff dunovo.xml @ 0:f875256c722e draft

planemo upload for repository https://github.com/galaxyproject/dunovo commit b'd00f828e5768c5fac3e382b9d12f34bbdf9019e9\n'-dirty

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/dunovo.xml	Sat Feb 18 05:58:44 2017 -0500
@@ -0,0 +1,75 @@
+<?xml version="1.0"?>
+<tool id="dunovo" name="Du Novo: Make consensus reads" version="0.7">
+  <description>from duplex sequencing alignments</description>
+  <requirements>
+    <requirement type="package" version="0.7">dunovo</requirement>
+    <!-- TODO: require Python 2.7 -->
+  </requirements>
+  <command detect_errors="exit_code">dunovo.sh -r $min_reads -q $qual_thres -F $qual_format '$input' '$dcs1' '$dcs2'
+    #if $keep_sscs:
+      '$sscs1' '$sscs2'
+    #end if
+  </command>
+  <inputs>
+    <param name="input" type="data" format="tabular" label="Aligned input reads" />
+    <param name="min_reads" type="integer" value="3" min="1" label="Minimum reads per family" help="Single-strand families with fewer than this many reads will be skipped."/>
+    <param name="qual_thres" type="integer" value="25" min="1" label="Minimum base quality" help="Bases with a PHRED score less than this will not be counted in the consensus making."/>
+    <param name="qual_format" type="select" label="FASTQ format" help="Solexa should also work for Illumina 1.3+ and 1.5+, and Sanger should work for Illumina 1.8+">
+      <option value="sanger" selected="true">Sanger (PHRED 0 = &quot;!&quot;)</option>
+      <option value="solexa">Solexa (PHRED 0 = &quot;@&quot;)</option>
+    </param>
+    <param name="keep_sscs" type="boolean" truevalue="true" falsevalue="" label="Output single-strand consensus sequences as well" />
+  </inputs>
+  <outputs>
+    <data name="dcs1" format="fasta" label="$tool.name on $on_string (mate 1)"/>
+    <data name="dcs2" format="fasta" label="$tool.name on $on_string (mate 2)"/>
+    <data name="sscs1" format="fasta" label="$tool.name on $on_string (SSCS mate 1)">
+      <filter>keep_sscs</filter>
+    </data>
+    <data name="sscs2" format="fasta" label="$tool.name on $on_string (SSCS mate 2)">
+      <filter>keep_sscs</filter>
+    </data>
+  </outputs>
+  <tests>
+    <test>
+      <param name="input" value="families.msa.tsv"/>
+      <output name="dcs1" file="families.cons_1.fa"/>
+      <output name="dcs2" file="families.cons_2.fa"/>
+    </test>
+  </tests>
+  <citations>
+    <citation type="bibtex">@article{Stoler2016,
+      author = {Stoler, Nicholas and Arbeithuber, Barbara and Guiblet, Wilfried and Makova, Kateryna D and Nekrutenko, Anton},
+      doi = {10.1186/s13059-016-1039-4},
+      issn = {1474-760X},
+      journal = {Genome biology},
+      number = {1},
+      pages = {180},
+      pmid = {27566673},
+      publisher = {Genome Biology},
+      title = {{Streamlined analysis of duplex sequencing data with Du Novo.}},
+      url = {http://www.ncbi.nlm.nih.gov/pubmed/27566673},
+      volume = {17},
+      year = {2016}
+    }</citation>
+  </citations>
+  <help>
+
+**What it does**
+
+This is for processing duplex sequencing data. It creates single-strand and duplex consensus reads from aligned read families.
+
+-----
+
+**Input**
+
+This expects the output format of the "Align families" tool.
+
+-----
+
+**Output**
+
+This will output final, duplex consensus reads in two FASTA files (first and second reads in the pairs). Optionally, you can save the single-strand reads too, in a separate FASTA file.
+
+    </help>
+</tool>