comparison junction_annotation.xml @ 51:09846d5169fa draft

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83

50:f242ee103277

<tool id="rseqc_junction_annotation" name="Junction Annotation" version="2.4galaxy1">
    <description>compares detected splice junctions to reference gene model</description>

    <macros>
        <import>rseqc_macros.xml</import>
    </macros>

    <requirements>
        <expand macro="requirement_package_r" />
        <expand macro="requirement_package_numpy" />
        <expand macro="requirement_package_rseqc" />
    </requirements>

    <expand macro="stdio" />

    <version_command><![CDATA[junction_annotation.py --version]]></version_command>

    <command><![CDATA[
        junction_annotation.py
            --input-file $input
            --refgene $refgene
            --out-prefix output
            --min-intron $min_intron
            --mapq $mapq
        ]]>
    </command>

    <inputs>
        <param name="input" type="data" format="bam,sam" label="input bam/sam file" help="(--input-file)"/>
        <param name="refgene" type="data" format="bed" label="reference gene model" help="(--refgene)"/>
        <param name="min_intron" type="integer" value="50" label="Minimum intron length (bp, default=50)" help="(--min-intron)" />
        <param name="mapq" type="integer" label="Minimum mapping quality (default=30)" help="Minimum phred scale mapping quality to consider a read 'uniquely mapped' (--mapq)" value="30" />
    </inputs>

    <outputs>
        <data format="xls" name="outputxls" from_work_dir="output.junction.xls" label="${tool.name} on ${on_string} (XLS)"/>
        <data format="txt" name="outputr" from_work_dir="output.junction_plot.r" label="${tool.name} on ${on_string} (R Script)" />
        <data format="pdf" name="outputpdf" from_work_dir="output.splice_events.pdf" label="${tool.name} on ${on_string} (Splice Events PDF)"/>
        <data format="pdf" name="outputjpdf" from_work_dir="output.splice_junction.pdf" label="${tool.name} on ${on_string} (Splice Junction PDF)" />
    </outputs>

    <tests>
        <test>
            <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/>
            <param name="refgene" value="hg19_RefSeq_chr1_1-100000.bed"/>
            <output name="outputxls" file="output.junction.xls"/>
            <output name="outputr" file="output.junction_plot.r"/>
            <output name="outputpdf" file="output.splice_events.pdf"/>
            <output name="outputjpdf" file="output.splice_junction.pdf"/>
        </test>
    </tests>

    <help><![CDATA[
junction_annotation.py

    - 'annotated', 'complete_novel' or 'partial_novel'.
2. output.anno.junction_plot.r: R script to generate pie chart
3. output.splice_junction.pdf: plot of splice junctions
4. output.splice_events.pdf: plot of splice events

.. image:: http://rseqc.sourceforge.net/_images/junction.png
   :height: 400 px
   :width: 850 px
   :scale: 80 %

-----

About RSeQC
+++++++++++

The RSeQC_ package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. "Basic modules" quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while "RNA-seq specific modules" investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation.

The RSeQC package is licensed under the GNU GPL v3 license.

.. image:: http://rseqc.sourceforge.net/_static/logo.png

.. _RSeQC: http://rseqc.sourceforge.net/
]]>
    </help>

    <expand macro="citations" />

51:09846d5169fa

<tool id="rseqc_junction_annotation" name="Junction Annotation" version="@WRAPPER_VERSION@">
    <description>compares detected splice junctions to reference gene model</description>

    <macros>
        <import>rseqc_macros.xml</import>
    </macros>

    <expand macro="requirements" />

    <expand macro="stdio" />

    <version_command><![CDATA[junction_annotation.py --version]]></version_command>

    <command><![CDATA[
        junction_annotation.py
            --input-file '${input}'
            --refgene '${refgene}'
            --out-prefix output
            --min-intron ${min_intron}
            --mapq ${mapq}
        ]]>
    </command>

    <inputs>
        <expand macro="bam_sam_param" />
        <expand macro="refgene_param" />
        <expand macro="min_intron_param" />
        <expand macro="mapq_param" />
        <expand macro="rscript_output_param" />
    </inputs>

    <outputs>
        <data format="pdf" name="outputpdf" from_work_dir="output.splice_events.pdf" label="${tool.name} on ${on_string} (Splice Events pdf)"/>
        <data format="pdf" name="outputjpdf" from_work_dir="output.splice_junction.pdf" label="${tool.name} on ${on_string} (Splice Junction pdf)" />
        <expand macro="xls_output_data" filename="output.junction.xls" />
        <expand macro="rscript_output_data" filename="output.junction_plot.r" />
    </outputs>

    <tests>
        <test>
            <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" />
            <param name="refgene" value="hg19_RefSeq_chr1_1-100000.bed" />
            <param name="rscript_output" value="true" />
            <output name="outputxls" file="output.junction.xls" />
            <output name="outputr" file="output.junction_plot.r" />
            <output name="outputpdf" file="output.splice_events.pdf" compare="sim_size" />
            <output name="outputjpdf" file="output.splice_junction.pdf" compare="sim_size" />
        </test>
    </tests>

    <help><![CDATA[
junction_annotation.py

    - 'annotated', 'complete_novel' or 'partial_novel'.
2. output.anno.junction_plot.r: R script to generate pie chart
3. output.splice_junction.pdf: plot of splice junctions
4. output.splice_events.pdf: plot of splice events

.. image:: $PATH_TO_IMAGES/junction.png
   :height: 400 px
   :width: 850 px
   :scale: 80 %

@ABOUT@

]]>
    </help>

    <expand macro="citations" />