Mercurial > repos > nml > fasta_extract
view fa-extract-few.pl @ 4:c96a4314df39 draft
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author | nml |
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date | Mon, 06 Feb 2017 16:35:50 -0500 |
parents | 75e70a6d8d60 |
children | 21888a4371d1 |
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#!/usr/bin/perl -w use strict; use Bio::SeqIO; my(@Options, $verbose, $inverse, $file,$list,$exact); setOptions(); my $in = Bio::SeqIO->new(-file=>$file, -format=>'Fasta'); my $out = Bio::SeqIO->new(-fh=>\*STDOUT, -format=>'Fasta'); my $nread=0; my $nwrote=0; my $pattern = join('|', @ARGV); if ( $list) { my @list; open my $in,'<',$list; while ( <$in>) { chomp; push @list,$_; } close $in; $pattern = join ('|',@list); } while (my $seq = $in->next_seq) { $nread++; my $match = ($seq->description =~ m/($pattern)/ or $seq->display_id =~ m/($pattern)/); if ($exact) { $match = ($seq->display_id =~ m/^($pattern)$/); } #print STDERR "Found match: ",$seq->display_id, " ", $seq->description, "\n" if $verbose; if ($match ^ $inverse) { # rare use for XOR ! $out->write_seq($seq); $nwrote++; } } #print STDERR "Read $nread sequences, wrote $nwrote, with pattern: $pattern\n"; exit(0); #---------------------------------------------------------------------- # Option setting routines sub setOptions { use Getopt::Long; @Options = ( {OPT=>"h|help", VAR=>\&usage, DESC=>"This help"}, {OPT=>"verbose!", VAR=>\$verbose, DEFAULT=>0, DESC=>"Verbose"}, {OPT=>"v|inverse!", VAR=>\$inverse, DEFAULT=>0, DESC=>"Output NON-matching sequences instead"}, {OPT=>"f|file=s", VAR=>\$file, DEFAULT=>"", DESC=>"The fasta file to extract sequences from"}, {OPT=>"exact", VAR=>\$exact, DEFAULT=>"", DESC=>"Exact matches for display id only"}, {OPT=>"l|list=s", VAR=>\$list, DEFAULT=>"", DESC=>"List of pattern to look from"}, ); (!@ARGV) && (usage()); &GetOptions(map {$_->{OPT}, $_->{VAR}} @Options) || usage(); # Now setup default values. foreach (@Options) { if (defined($_->{DEFAULT}) && !defined(${$_->{VAR}})) { ${$_->{VAR}} = $_->{DEFAULT}; } } } sub usage { print "Usage: $0 [options] id1 [id2 ...] < input.fasta > output.fasta\n"; foreach (@Options) { printf " --%-13s %s%s.\n",$_->{OPT},$_->{DESC}, defined($_->{DEFAULT}) ? " (default '$_->{DEFAULT}')" : ""; } exit(1); } #----------------------------------------------------------------------