# HG changeset patch # User nml # Date 1655755596 0 # Node ID 18aad692772aec5304294af20e6652e4c9a6c812 # Parent 9def47f3c1e48dc8b7131ec50f69ca8a66ee009a planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e diff -r 9def47f3c1e4 -r 18aad692772a aacoverage.xml --- a/aacoverage.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/aacoverage.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,13 +1,15 @@ - + Builds an aa census and returns its coverage - - quasitools - + + macros.xml + + @@ -44,14 +46,5 @@ A file with one entry per line with the AA position and the coverage at the position. ]]> - - - @misc{GitHubquasitoolsaacoverage, - title = {quasitools aacoverage}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a callaavar.xml --- a/callaavar.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/callaavar.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,12 +1,15 @@ - + Identifies amino acid mutations - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolscallaavar, - title = {quasitools callaavar}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a callcodonvar.xml --- a/callcodonvar.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/callcodonvar.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,14 +1,17 @@ - + Identifies codon variants and non-synonymous/synonymous mutations - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolscallcodonvar, - title = {quasitools callcodonvar}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a callntvar.xml --- a/callntvar.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/callntvar.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,12 +1,15 @@ - + Identifies nucleotide variants - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolscallntvar, - title = {quasitools callntvar}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a complexity_bam.xml --- a/complexity_bam.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/complexity_bam.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,12 +1,14 @@ - - - quasitools - + + + macros.xml + + @@ -44,14 +46,5 @@ ]]> - - - @misc{GitHubquasitoolscomplexity, - title = {quasitools complexity}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a complexity_fasta.xml --- a/complexity_fasta.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/complexity_fasta.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,7 +1,8 @@ - - - quasitools - + + + macros.xml + + - - - @misc{GitHubquasitoolscomplexity, - title = {quasitools complexity}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a consensus.xml --- a/consensus.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/consensus.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,12 +1,15 @@ - + Generate a consensus sequence from a BAM file - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolsconsensus, - title = {quasitools consensus}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a distance.xml --- a/distance.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/distance.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,8 +1,9 @@ - + Calculate the evolutionary distance between viral quasispecies. - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolsdistance, - title = {quasitools distance}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a dnds.xml --- a/dnds.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/dnds.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,8 +1,9 @@ - + Calculate the dN/dS value for each region in a bed file - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolsdnds, - title = {quasitools dnds}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a drmutations.xml --- a/drmutations.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/drmutations.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,12 +1,15 @@ - + - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolsdrmutations, - title = {quasitools drmutations}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a hydra.xml --- a/hydra.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/hydra.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,8 +1,9 @@ - + Identifies drug resistance within an NGS dataset - - quasitools - + + macros.xml + + - + consensus['consensus_bool'] == "true_consensus" @@ -221,9 +222,9 @@ - - - + + + @@ -280,7 +281,7 @@ * dr_report.csv * filtered.fastq * hydra.vcf - * mutation_report.hmcf + * mutation_report.aavf * stats.txt The description of each of these directories/files are as follows: @@ -292,7 +293,7 @@ * __dr_report.csv__: A report detailing the drug resistant mutations found, above the reporting threshold (default: 1%). * __filtered.fastq__: The reads remaining after the filtering stage. * __hydra.vcf__: The variants found by the pipeline. - * __mutation_report.hmcf__: The AA mutations found by the pipeline. + * __mutation_report.aavf__: The AA mutations found by the pipeline. * __stats.txt__: A log file detailing size after filtering and major stages. The __dr_report.csv__ file lists all found drug resistant mutations (mutations included in the mutation database) which have frequency greater than the reporting threshold. An example of this file is given below. @@ -302,30 +303,23 @@ Gene,Category,Surveillance,Wildtype,Position,Mutation,Mutation Frequency,Coverage RT,NNRTI,Yes,K,103,N,9.03,155 -The __mutation_report.hmcf__ files is our custom VCF like file which details all of the AA mutations found by the pipeline. An example if this file is given below. +The __mutation_report.aavf__ file is in AAVF format (https://github.com/winhiv/aavf-spec), an amino acid variant format inspired by the VCF format. The __mutation_report.aavf__ file details all of the AA mutations found by the pipeline. An example if this file is given below. -Example: __mutation_report.hmcf__ +Example: __mutation_report.aavf__ - ##fileformat=HMCFv1 - ##fileDate=20150008 - ##source=HyDRA - ##reference=/home/ericenns/hydra/var/hxb2_pol.fas - ##INFO= - ##INFO= - ##INFO= - ##FILTER= - #GENE CATEGORY SURVEILLANCE TYPE WILDTYPE POS MUTANT FILTER MUTANT_FREQ COVERAGE INFO - RT NNRTI Yes mutation K 103 N PASS 0.0903 155 WC=aaa;MC=aaC;MCF=0.0903 + ##fileformat=AAVFv1.0 + ##fileDate=20220615 + ##source=quasitools:hydra + ##reference=hxb2_pol.fas + ##INFO= + ##INFO= + ##INFO= + ##INFO= + ##INFO= + ##FILTER= + #CHROM GENE POS REF ALT FILTER ALT_FREQ COVERAGE INFO + hxb2_pol RT 101 K P PASS 0.1423 1574 RC=aaa;AC=CCa;ACF=0.1423;CAT=NNRTI;SRVL=Yes ]]> - - - @misc{GitHubquasitoolshydra, - title = {quasitools hydra}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - + diff -r 9def47f3c1e4 -r 18aad692772a macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Mon Jun 20 20:06:36 2022 +0000 @@ -0,0 +1,22 @@ + + + + + quasitools + + + 0.7.0 + 1 + + + + @misc{GitHubquasitools, + title = {quasitools}, + publisher = {phac-nml}, + journal = {GitHub repository}, + url = {https://github.com/phac-nml/quasitools}, + } + + + + diff -r 9def47f3c1e4 -r 18aad692772a quality.xml --- a/quality.xml Tue Aug 06 08:50:28 2019 -0400 +++ b/quality.xml Mon Jun 20 20:06:36 2022 +0000 @@ -1,8 +1,9 @@ - + Performs quality control on FASTQ reads. - - quasitools - + + macros.xml + + - - - @misc{GitHubquasitoolsquality, - title = {quasitools quality}, - publisher = {phac-nml}, - journal = {GitHub repository}, - url = {https://github.com/phac-nml/quasitools}, - } - - +