comparison staramr_search.xml @ 18:3b22de20bd4b draft default tip

planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 1a2d7c4c5b7c0d3aac10d1d5fbd8d33550f840ed
author nml
date Tue, 19 Sep 2023 18:41:35 +0000
parents 141afacabcfa
children
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17:141afacabcfa 18:3b22de20bd4b
1 <tool id="staramr_search" name="staramr" version="@VERSION@+galaxy0" profile="16.04"> 1 <tool id="staramr_search" name="staramr" version="@VERSION@+galaxy1" profile="16.04">
2 <description>Scans genome assemblies against the ResFinder, PlasmidFinder, and PointFinder databases searching for AMR genes.</description> 2 <description>Scans genome assemblies against the ResFinder, PlasmidFinder, and PointFinder databases searching for AMR genes.</description>
3 <macros> 3 <macros>
4 <token name="@VERSION@">0.10.0</token> 4 <token name="@VERSION@">0.10.0</token>
5 </macros> 5 </macros>
6 <requirements> 6 <requirements>
417 417
418 Usage 418 Usage
419 ----- 419 -----
420 420
421 1. Select your genome contigs (in FASTA format). 421 1. Select your genome contigs (in FASTA format).
422 2. Select whether or not you wish to scan your genome for point mutations giving antimicrobial resistance using the PointFinder database. This requires you to specify the specific organism you are scanning (currently only *salmonella* and *campylobacter* is supported). 422 2. Select whether or not you wish to scan your genome for point mutations giving antimicrobial resistance using the PointFinder database. This requires you to specify the specific organism you are scanning.
423 3. Run the tool. 423 3. Run the tool.
424 424
425 Input 425 Input
426 ----- 426 -----
427 427
439 439
440 #gene_id 440 #gene_id
441 aac(6')-Iaa_1_NC_003197 441 aac(6')-Iaa_1_NC_003197
442 ColpVC_1__JX133088 442 ColpVC_1__JX133088
443 443
444 Complex mutations file
445 ``````````````````````
446
447 Complex mutations describe multiple point mutations that must be simultaneously present in order to confer resistance. Complex mutations may be specified by the user using a TSV-formatted file with the following format:
448
449 ::
450
451 positions mandatory phenotype
452 mutation(s) mutation(s) phenotype
453
454 Where "positions" are all the point mutations to group into the complex mutation (optional and mandatory), "mandatory" are all the point mutations that must be present for the complex mutation to be reported (mandatory is a subset of positions), and phenotype is the phenotype that is conferred when this set of mutations is present.
455
444 Output 456 Output
445 ------ 457 ------
446 458
447 There are 6 different output files produced by `staramr` as well as a collection of additional files. 459 There are 6 different output files produced by `staramr` as well as a collection of additional files.
448 460
449 mlst.tsv 461 mlst.tsv
450 ```````````````````` 462 ````````````````````
451 463
452 A tabular file of each multi-locus sequence type (MLST) and it's corresponding locus/alleles, one genome per line. 464 A tabular file of each multi-locus sequence type (MLST) and it's corresponding locus/alleles, one genome per line.
453 465
454 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+ 466 +------------+---------------------+---------------+---------+---------+---------+---------+---------+---------+----------+
455 | Isolate ID | Scheme | Sequence Type | Locus 1 | Locus 2 | Locus 3 | Locus 4 | Locus 5 | Locus 6 | Locus 7 | 467 | Isolate ID | Scheme | Sequence Type | Locus 1 | Locus 2 | Locus 3 | Locus 4 | Locus 5 | Locus 6 | Locus 7 |
456 +============+==============+===================================+============+============+============+============+============+============+============+ 468 +============+=====================+===============+=========+=========+=========+=========+=========+=========+==========+
457 | SRR1952908 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | 469 | SRR1952908 | senterica_achtman_2 | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) |
458 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+---+--------+ 470 | SRR1952926 | senterica_achtman_2 | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) |
459 | SRR1952926 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | 471 +------------+---------------------+---------------+---------+---------+---------+---------+---------+---------+----------+
460 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+ 472
461 473
462 474
463 summary.tsv 475 summary.tsv
464 ``````````` 476 ```````````
465 477
466 A summary of all detected AMR genes/mutations in each genome, one genome per line. 478 A summary of all detected AMR genes/mutations in each genome, one genome per line.
467 479
468 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ 480
469 | Isolate ID | Quality Module | Genotype | Predicted Phenotype | Plasmid | Scheme | Sequence Type | Genome Length | N50 value | Number of Contigs Greater Than Or Equal To 300 bp | Quality Module Feedback | 481 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------+----------------------------------------------+---------------------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+
470 +============+================+===========================================================+===========================================================================================================+=====================================+===========+===============+===============+===========+===================================================+=========================+ 482 | Isolate ID | Quality Module | Genotype | Predicted Phenotype | CGE Predicted Phenotype | Plasmid | Scheme | Sequence Type | Genome Length | N50 value | Number of Contigs Greater Than Or Equal To 300 bp | Quality Module Feedback |
471 | SRR1952908 | Passed | aadA1, aadA2, blaTEM-57, cmlA1, gyrA (S83Y), sul3, tet(A) | streptomycin, ampicillin, chloramphenicol, ciprofloxacin I/R, nalidixic acid, sulfisoxazole, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4796082 | 225419 | 59 | | 483 +============+================+===========================================================+===========================================================================================================+===========================================================================================================================================================================================+==============================================+=====================+===============+===============+===========+===================================================+=========================+
472 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ 484 | SRR1952908 | Passed | aadA1, aadA2, blaTEM-57, cmlA1, gyrA (S83Y), sul3, tet(A) | streptomycin, ampicillin, chloramphenicol, ciprofloxacin I/R, nalidixic acid, sulfisoxazole, tetracycline | Spectinomycin, Streptomycin, Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin, Chloramphenicol, Nalidixic acid, Ciprofloxacin, Sulfamethoxazole, Doxycycline, Tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1-I(Alpha) | senterica_achtman_2 | 11 | 4785500 | 250423 | 41 | |
473 | SRR1952926 | Passed | blaTEM-57, gyrA (S83Y), tet(A) | ampicillin, ciprofloxacin I/R, nalidixic acid, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4794071 | 225380 | 50 | | 485 | SRR1952926 | Passed | blaTEM-57, gyrA (S83Y), tet(A) | ampicillin, ciprofloxacin I/R, nalidixic acid, tetracycline | Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin, Nalidixic acid, Ciprofloxacin, Doxycycline, Tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1-I(Alpha) | senterica_achtman_2 | 11 | 4785451 | 228311 | 40 | |
474 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ 486 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------+----------------------------------------------+---------------------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+
475 487
476 488
477 detailed_summary.tsv 489 detailed_summary.tsv
478 ```````````````````` 490 ````````````````````
479 491
480 A detailed summary of all detected AMR genes/mutations/plasmids in each genome/type, one gene/type per line. 492 A detailed summary of all detected AMR genes/mutations/plasmids in each genome/type, one gene/type per line.
481 493
482 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ 494 +------------+----------------------------+------------+---------------------+-----------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+
483 | Isolate ID | Gene/Plasmid | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | Data Type | 495 | Isolate ID | Data | Data Type | Predicted Phenotype | CGE Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession |
484 +============+==============+===================================+===========+==========+=========================+=============+========+========+===========+============+ 496 +============+============================+============+=====================+=============================+===========+==========+=========================+=============+=======+======+===========+
485 | SRR1952908 | ColpVC | | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | Plasmid | 497 | SRR1952908 | ST11 (senterica_achtman_2) | MLST | | | | | | | | | |
486 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ 498 | SRR1952908 | ColpVC | Plasmid | | | 98.96 | 100.0 | 193/193 | contig00038 | 1618 | 1426 | JX133088 |
487 | SRR1952908 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | Plasmid | 499 | SRR1952908 | aadA1 | Resistance | streptomycin | Spectinomycin, Streptomycin | 100.0 | 100.0 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 |
488 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ 500 +------------+----------------------------+------------+---------------------+-----------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+
489 | SRR1952908 | IncFII(S) | | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 | Plasmid | 501
490 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
491 | SRR1952908 | IncI1 | | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 | Plasmid |
492 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
493 | SRR1952908 | aadA1 | streptomycin | 100 | 100 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 | Resistance |
494 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
495 | SRR1952908 | aadA2 | streptomycin | 100 | 100 | 780/780 | contig00030 | 7748 | 6969 | X68227 | Resistance |
496 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
497 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 | Resistance |
498 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
499 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 | Resistance |
500 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
501 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00008 | 22801 | 20165 | | Resistance |
502 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
503 | SRR1952908 | sul3 | sulfisoxazole | 100 | 100 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | Resistance |
504 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
505 | SRR1952908 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00032 | 1476 | 2722 | AF534183 | Resistance |
506 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
507 | SRR1952926 | ColpVC | | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 | Plasmid |
508 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
509 | SRR1952926 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 | Plasmid |
510 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
511 | SRR1952926 | IncFII(S) | | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 | Plasmid |
512 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
513 | SRR1952926 | IncI1 | | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 | Plasmid |
514 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
515 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 | Resistance |
516 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
517 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00011 | 157768 | 160404 | | Resistance |
518 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
519 | SRR1952926 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00027 | 1405 | 2651 | AF534183 | Resistance |
520 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+
521 502
522 503
523 resfinder.tsv 504 resfinder.tsv
524 ````````````` 505 `````````````
525 506
526 A tabular file of each AMR gene and additional BLAST information from the **ResFinder** database, one gene per line. 507 A tabular file of each AMR gene and additional BLAST information from the **ResFinder** database, one gene per line.
527 508
528 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ 509 +------------+--------+---------------------+---------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+-----------+-----------+
529 | Isolate ID | Gene | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | 510 | Isolate ID | Gene | Predicted Phenotype | CGE Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | Sequence | CGE Notes |
530 +============+============+======================+============+===========+==========================+==============+========+=======+===========+ 511 +============+========+=====================+===========================+===========+==========+=========================+=============+=======+======+===========+===========+===========+
531 | SRR1952908 | sul3 | sulfisoxazole | 100.00 | 100.00 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | 512 | SRR1952908 | sul3 | sulfisoxazole | Sulfamethoxazole | 100.00 | 100.00 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | ATGA[...] | |
532 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ 513 | SRR1952908 | tet(A) | tetracycline | Doxycycline, Tetracycline | 99.92 | 97.80 | 1247/1275 | contig00032 | 1476 | 2722 | AF534183 | ATGT[...] | |
533 | SRR1952908 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00032 | 1551 | 2750 | AJ517790 | 514 +------------+--------+---------------------+---------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+-----------+-----------+
534 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ 515
535 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100.00 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 |
536 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
537 | SRR1952908 | aadA1 | streptomycin | 100.00 | 100.00 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 |
538 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
539 | SRR1952908 | aadA2 | streptomycin | 99.75 | 100.00 | 792/792 | contig00030 | 7760 | 6969 | JQ364967 |
540 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
541 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 |
542 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
543 | SRR1952926 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00027 | 1480 | 2679 | AJ517790 |
544 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
545 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 |
546 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+
547 516
548 plasmidfinder.tsv 517 plasmidfinder.tsv
549 ````````````````` 518 `````````````````
550 519
551 A tabular file of each AMR plasmid type and additional BLAST information from the **PlasmidFinder** database, one plasmid type per line. 520 A tabular file of each AMR plasmid type and additional BLAST information from the **PlasmidFinder** database, one plasmid type per line.
552 521
553 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ 522 +------------+-----------+-----------+----------+-------------------------+-------------+-------+------+-----------+
554 | Isolate ID | Plasmid | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | 523 | Isolate ID | Plasmid | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession |
555 +============+===========+===========+==========+=========================+=============+=======+=======+===========+ 524 +============+===========+===========+==========+=========================+=============+=======+======+===========+
556 | SRR1952908 | ColpVC | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | 525 | SRR1952908 | ColpVC | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 |
557 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ 526 | SRR1952908 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 |
558 | SRR1952908 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | 527 +------------+-----------+-----------+----------+-------------------------+-------------+-------+------+-----------+
559 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ 528
560 | SRR1952908 | IncFII(S) | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 |
561 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
562 | SRR1952908 | IncI1 | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 |
563 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
564 | SRR1952926 | ColpVC | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 |
565 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
566 | SRR1952926 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 |
567 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
568 | SRR1952926 | IncFII(S) | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 |
569 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
570 | SRR1952926 | IncI1 | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 |
571 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+
572 529
573 pointfinder.tsv 530 pointfinder.tsv
574 ``````````````` 531 ```````````````
575 532
576 A tabular file of each AMR point mutation and additional BLAST information from the **PointFinder** database, one gene per line. 533 A tabular file of each AMR point mutation and additional BLAST information from the **PointFinder** database, one gene per line.
577 534
578 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ 535 +------------+-------------+-----------------------------------+------------------------------+-------+----------+---------------------+-----------+----------+-------------------------+-------------+--------+--------+----------------------+-----------+-----------------------+---------------------+------------------+
579 | Isolate ID | Gene | Predicted Phenotype | Type | Position | Mutation | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | 536 | Isolate ID | Gene | Predicted Phenotype | CGE Predicted Phenotype | Type | Position | Mutation | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Pointfinder Position | CGE Notes | CGE Required Mutation | CGE Mechanism | CGE PMID |
580 +=============+==============+====================================+========+===========+======================+============+===========+==========================+==============+=========+========+ 537 +============+=============+===================================+==============================+=======+==========+=====================+===========+==========+=========================+=============+========+========+======================+===========+=======================+=====================+==================+
581 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00008 | 22801 | 20165 | 538 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | Nalidixic acid,Ciprofloxacin | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00008 | 22801 | 20165 | S83Y | | | Target modification | 7492118,10471553 |
582 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ 539 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | Nalidixic acid,Ciprofloxacin | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00011 | 157768 | 160404 | S83Y | | | Target modification | 7492118,10471553 |
583 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00011 | 157768 | 160404 | 540 +------------+-------------+-----------------------------------+------------------------------+-------+----------+---------------------+-----------+----------+-------------------------+-------------+--------+--------+----------------------+-----------+-----------------------+---------------------+------------------+
584 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ 541
585 542
586 settings.txt 543 settings.txt
587 ```````````` 544 ````````````
588 545
589 The command-line, database versions, and other settings used to run `staramr`. 546 The command-line, database versions, and other settings used to run `staramr`.
590 547
591 :: 548 ::
592 549
593 command_line = staramr search -o out --pointfinder-organism salmonella SRR1952908.fasta SRR1952926.fasta 550 command_line = staramr search --pointfinder-organism enterococcus_faecium -o out pbp5.fa
594 version = 0.7.1 551 version = 0.10.0
595 start_time = 2019-04-25 16:01:32 552 start_time = 2023-08-23 13:37:41
596 end_time = 2019-04-25 16:01:34 553 end_time = 2023-08-23 13:37:42
597 total_minutes = 0.04 554 total_minutes = 0.02
598 resfinder_db_dir = staramr/databases/data/dist/resfinder 555 resfinder_db_dir = staramr/databases/data/dist/resfinder
599 resfinder_db_url = https://bitbucket.org/genomicepidemiology/resfinder_db.git 556 resfinder_db_url = https://bitbucket.org/genomicepidemiology/resfinder_db.git
600 resfinder_db_commit = e8f1eb2585cd9610c4034a54ce7fc4f93aa95535 557 resfinder_db_commit = fa32d9a3cf0c12ec70ca4e90c45c0d590ee810bd
601 resfinder_db_date = Mon, 16 Jul 2018 16:58 558 resfinder_db_date = Tue, 24 May 2022 06:51
602 pointfinder_db_dir = staramr/databases/data/dist/pointfinder 559 pointfinder_db_dir = staramr/databases/data/dist/pointfinder
603 pointfinder_db_url = https://bitbucket.org/genomicepidemiology/pointfinder_db.git 560 pointfinder_db_url = https://bitbucket.org/genomicepidemiology/pointfinder_db.git
604 pointfinder_db_commit = 8706a6363bb29e47e0e398c53043b037c24b99a7 561 pointfinder_db_commit = 8c694b9f336153e6d618b897b3b4930961521eb8
605 pointfinder_db_date = Wed, 04 Jul 2018 14:27 562 pointfinder_db_date = Mon, 01 Feb 2021 15:46
563 pointfinder_organisms_all = campylobacter, enterococcus_faecalis, enterococcus_faecium, escherichia_coli, helicobacter_pylori, klebsiella, mycobacterium_tuberculosis, neisseria_gonorrhoeae, plasmodium_falciparum, salmonella, staphylococcus_aureus
564 pointfinder_organisms_valid = campylobacter, enterococcus_faecalis, enterococcus_faecium, escherichia_coli, helicobacter_pylori, salmonella
606 plasmidfinder_db_dir = staramr/databases/data/dist/plasmidfinder 565 plasmidfinder_db_dir = staramr/databases/data/dist/plasmidfinder
607 plasmidfinder_db_url = https://bitbucket.org/genomicepidemiology/plasmidfinder_db.git 566 plasmidfinder_db_url = https://bitbucket.org/genomicepidemiology/plasmidfinder_db.git
608 plasmidfinder_db_commit = 81919954cbedaff39056610ab584ab4c06011ed8 567 plasmidfinder_db_commit = c18e08c17a5988d4f075fc1171636e47546a323d
609 plasmidfinder_db_date = Tue, 20 Nov 2018 08:51 568 plasmidfinder_db_date = Wed, 18 Jan 2023 09:45
610 pointfinder_gene_drug_version = 050218 569 mlst_version = 2.23.0
611 resfinder_gene_drug_version = 050218.1 570 pointfinder_organism = enterococcus_faecium
571 pointfinder_gene_drug_version = 072621.2
572 resfinder_gene_drug_version = 072621
612 573
613 results.xlsx 574 results.xlsx
614 ```````````` 575 ````````````
615 576
616 An Excel spreadsheet containing the previous 5 files as separate worksheets. 577 An Excel spreadsheet containing the previous 5 files as separate worksheets.
618 BLAST Hits 579 BLAST Hits
619 `````````` 580 ``````````
620 581
621 The dataset collection **hits** stores fasta files of the specific blast hits. 582 The dataset collection **hits** stores fasta files of the specific blast hits.
622 583
623 Galaxy wrapper written by Aaron Petkau at the National Microbiology Laboratory, Public Health Agency of Canada. 584 Galaxy wrapper written by Aaron Petkau and Eric Marinier at the National Microbiology Laboratory, Public Health Agency of Canada.
624 585
625 .. _staramr: https://github.com/phac-nml/staramr 586 .. _staramr: https://github.com/phac-nml/staramr
626 .. _ResFinder: https://bitbucket.org/genomicepidemiology/resfinder_db 587 .. _ResFinder: https://bitbucket.org/genomicepidemiology/resfinder_db
627 .. _PlasmidFinder: https://bitbucket.org/genomicepidemiology/plasmidfinder_db 588 .. _PlasmidFinder: https://bitbucket.org/genomicepidemiology/plasmidfinder_db
628 .. _PointFinder: https://bitbucket.org/genomicepidemiology/pointfinder_db 589 .. _PointFinder: https://bitbucket.org/genomicepidemiology/pointfinder_db