Mercurial > repos > nml > staramr
comparison staramr_search.xml @ 18:3b22de20bd4b draft default tip
planemo upload for repository https://github.com/phac-nml/galaxy_tools/tree/master/tools/staramr commit 1a2d7c4c5b7c0d3aac10d1d5fbd8d33550f840ed
author | nml |
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date | Tue, 19 Sep 2023 18:41:35 +0000 |
parents | 141afacabcfa |
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17:141afacabcfa | 18:3b22de20bd4b |
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1 <tool id="staramr_search" name="staramr" version="@VERSION@+galaxy0" profile="16.04"> | 1 <tool id="staramr_search" name="staramr" version="@VERSION@+galaxy1" profile="16.04"> |
2 <description>Scans genome assemblies against the ResFinder, PlasmidFinder, and PointFinder databases searching for AMR genes.</description> | 2 <description>Scans genome assemblies against the ResFinder, PlasmidFinder, and PointFinder databases searching for AMR genes.</description> |
3 <macros> | 3 <macros> |
4 <token name="@VERSION@">0.10.0</token> | 4 <token name="@VERSION@">0.10.0</token> |
5 </macros> | 5 </macros> |
6 <requirements> | 6 <requirements> |
417 | 417 |
418 Usage | 418 Usage |
419 ----- | 419 ----- |
420 | 420 |
421 1. Select your genome contigs (in FASTA format). | 421 1. Select your genome contigs (in FASTA format). |
422 2. Select whether or not you wish to scan your genome for point mutations giving antimicrobial resistance using the PointFinder database. This requires you to specify the specific organism you are scanning (currently only *salmonella* and *campylobacter* is supported). | 422 2. Select whether or not you wish to scan your genome for point mutations giving antimicrobial resistance using the PointFinder database. This requires you to specify the specific organism you are scanning. |
423 3. Run the tool. | 423 3. Run the tool. |
424 | 424 |
425 Input | 425 Input |
426 ----- | 426 ----- |
427 | 427 |
439 | 439 |
440 #gene_id | 440 #gene_id |
441 aac(6')-Iaa_1_NC_003197 | 441 aac(6')-Iaa_1_NC_003197 |
442 ColpVC_1__JX133088 | 442 ColpVC_1__JX133088 |
443 | 443 |
444 Complex mutations file | |
445 `````````````````````` | |
446 | |
447 Complex mutations describe multiple point mutations that must be simultaneously present in order to confer resistance. Complex mutations may be specified by the user using a TSV-formatted file with the following format: | |
448 | |
449 :: | |
450 | |
451 positions mandatory phenotype | |
452 mutation(s) mutation(s) phenotype | |
453 | |
454 Where "positions" are all the point mutations to group into the complex mutation (optional and mandatory), "mandatory" are all the point mutations that must be present for the complex mutation to be reported (mandatory is a subset of positions), and phenotype is the phenotype that is conferred when this set of mutations is present. | |
455 | |
444 Output | 456 Output |
445 ------ | 457 ------ |
446 | 458 |
447 There are 6 different output files produced by `staramr` as well as a collection of additional files. | 459 There are 6 different output files produced by `staramr` as well as a collection of additional files. |
448 | 460 |
449 mlst.tsv | 461 mlst.tsv |
450 ```````````````````` | 462 ```````````````````` |
451 | 463 |
452 A tabular file of each multi-locus sequence type (MLST) and it's corresponding locus/alleles, one genome per line. | 464 A tabular file of each multi-locus sequence type (MLST) and it's corresponding locus/alleles, one genome per line. |
453 | 465 |
454 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+ | 466 +------------+---------------------+---------------+---------+---------+---------+---------+---------+---------+----------+ |
455 | Isolate ID | Scheme | Sequence Type | Locus 1 | Locus 2 | Locus 3 | Locus 4 | Locus 5 | Locus 6 | Locus 7 | | 467 | Isolate ID | Scheme | Sequence Type | Locus 1 | Locus 2 | Locus 3 | Locus 4 | Locus 5 | Locus 6 | Locus 7 | |
456 +============+==============+===================================+============+============+============+============+============+============+============+ | 468 +============+=====================+===============+=========+=========+=========+=========+=========+=========+==========+ |
457 | SRR1952908 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | | 469 | SRR1952908 | senterica_achtman_2 | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | |
458 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+---+--------+ | 470 | SRR1952926 | senterica_achtman_2 | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | |
459 | SRR1952926 | senterica | 11 | aroC(5) | dnaN(2) | hemD(3) | hisD(7) | purE(6) | sucA(6) | thrA(11) | | 471 +------------+---------------------+---------------+---------+---------+---------+---------+---------+---------+----------+ |
460 +------------+--------------+-----------------------------------+------------+------------+------------+------------+------------+------------+------------+ | 472 |
461 | 473 |
462 | 474 |
463 summary.tsv | 475 summary.tsv |
464 ``````````` | 476 ``````````` |
465 | 477 |
466 A summary of all detected AMR genes/mutations in each genome, one genome per line. | 478 A summary of all detected AMR genes/mutations in each genome, one genome per line. |
467 | 479 |
468 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ | 480 |
469 | Isolate ID | Quality Module | Genotype | Predicted Phenotype | Plasmid | Scheme | Sequence Type | Genome Length | N50 value | Number of Contigs Greater Than Or Equal To 300 bp | Quality Module Feedback | | 481 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------+----------------------------------------------+---------------------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ |
470 +============+================+===========================================================+===========================================================================================================+=====================================+===========+===============+===============+===========+===================================================+=========================+ | 482 | Isolate ID | Quality Module | Genotype | Predicted Phenotype | CGE Predicted Phenotype | Plasmid | Scheme | Sequence Type | Genome Length | N50 value | Number of Contigs Greater Than Or Equal To 300 bp | Quality Module Feedback | |
471 | SRR1952908 | Passed | aadA1, aadA2, blaTEM-57, cmlA1, gyrA (S83Y), sul3, tet(A) | streptomycin, ampicillin, chloramphenicol, ciprofloxacin I/R, nalidixic acid, sulfisoxazole, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4796082 | 225419 | 59 | | | 483 +============+================+===========================================================+===========================================================================================================+===========================================================================================================================================================================================+==============================================+=====================+===============+===============+===========+===================================================+=========================+ |
472 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ | 484 | SRR1952908 | Passed | aadA1, aadA2, blaTEM-57, cmlA1, gyrA (S83Y), sul3, tet(A) | streptomycin, ampicillin, chloramphenicol, ciprofloxacin I/R, nalidixic acid, sulfisoxazole, tetracycline | Spectinomycin, Streptomycin, Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin, Chloramphenicol, Nalidixic acid, Ciprofloxacin, Sulfamethoxazole, Doxycycline, Tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1-I(Alpha) | senterica_achtman_2 | 11 | 4785500 | 250423 | 41 | | |
473 | SRR1952926 | Passed | blaTEM-57, gyrA (S83Y), tet(A) | ampicillin, ciprofloxacin I/R, nalidixic acid, tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1 | senterica | 11 | 4794071 | 225380 | 50 | | | 485 | SRR1952926 | Passed | blaTEM-57, gyrA (S83Y), tet(A) | ampicillin, ciprofloxacin I/R, nalidixic acid, tetracycline | Amoxicillin, Ampicillin, Cephalothin, Piperacillin, Ticarcillin, Nalidixic acid, Ciprofloxacin, Doxycycline, Tetracycline | ColpVC, IncFIB(S), IncFII(S), IncI1-I(Alpha) | senterica_achtman_2 | 11 | 4785451 | 228311 | 40 | | |
474 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------+-----------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ | 486 +------------+----------------+-----------------------------------------------------------+-----------------------------------------------------------------------------------------------------------+-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------+----------------------------------------------+---------------------+---------------+---------------+-----------+---------------------------------------------------+-------------------------+ |
475 | 487 |
476 | 488 |
477 detailed_summary.tsv | 489 detailed_summary.tsv |
478 ```````````````````` | 490 ```````````````````` |
479 | 491 |
480 A detailed summary of all detected AMR genes/mutations/plasmids in each genome/type, one gene/type per line. | 492 A detailed summary of all detected AMR genes/mutations/plasmids in each genome/type, one gene/type per line. |
481 | 493 |
482 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | 494 +------------+----------------------------+------------+---------------------+-----------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+ |
483 | Isolate ID | Gene/Plasmid | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | Data Type | | 495 | Isolate ID | Data | Data Type | Predicted Phenotype | CGE Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | |
484 +============+==============+===================================+===========+==========+=========================+=============+========+========+===========+============+ | 496 +============+============================+============+=====================+=============================+===========+==========+=========================+=============+=======+======+===========+ |
485 | SRR1952908 | ColpVC | | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | Plasmid | | 497 | SRR1952908 | ST11 (senterica_achtman_2) | MLST | | | | | | | | | | |
486 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | 498 | SRR1952908 | ColpVC | Plasmid | | | 98.96 | 100.0 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | |
487 | SRR1952908 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | Plasmid | | 499 | SRR1952908 | aadA1 | Resistance | streptomycin | Spectinomycin, Streptomycin | 100.0 | 100.0 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 | |
488 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | 500 +------------+----------------------------+------------+---------------------+-----------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+ |
489 | SRR1952908 | IncFII(S) | | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 | Plasmid | | 501 |
490 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
491 | SRR1952908 | IncI1 | | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 | Plasmid | | |
492 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
493 | SRR1952908 | aadA1 | streptomycin | 100 | 100 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 | Resistance | | |
494 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
495 | SRR1952908 | aadA2 | streptomycin | 100 | 100 | 780/780 | contig00030 | 7748 | 6969 | X68227 | Resistance | | |
496 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
497 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 | Resistance | | |
498 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
499 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 | Resistance | | |
500 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
501 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00008 | 22801 | 20165 | | Resistance | | |
502 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
503 | SRR1952908 | sul3 | sulfisoxazole | 100 | 100 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | Resistance | | |
504 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
505 | SRR1952908 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00032 | 1476 | 2722 | AF534183 | Resistance | | |
506 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
507 | SRR1952926 | ColpVC | | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 | Plasmid | | |
508 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
509 | SRR1952926 | IncFIB(S) | | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 | Plasmid | | |
510 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
511 | SRR1952926 | IncFII(S) | | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 | Plasmid | | |
512 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
513 | SRR1952926 | IncI1 | | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 | Plasmid | | |
514 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
515 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 | Resistance | | |
516 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
517 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | 99.96 | 100 | 2637/2637 | contig00011 | 157768 | 160404 | | Resistance | | |
518 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
519 | SRR1952926 | tet(A) | tetracycline | 99.92 | 97.8 | 1247/1275 | contig00027 | 1405 | 2651 | AF534183 | Resistance | | |
520 +------------+--------------+-----------------------------------+-----------+----------+-------------------------+-------------+--------+--------+-----------+------------+ | |
521 | 502 |
522 | 503 |
523 resfinder.tsv | 504 resfinder.tsv |
524 ````````````` | 505 ````````````` |
525 | 506 |
526 A tabular file of each AMR gene and additional BLAST information from the **ResFinder** database, one gene per line. | 507 A tabular file of each AMR gene and additional BLAST information from the **ResFinder** database, one gene per line. |
527 | 508 |
528 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | 509 +------------+--------+---------------------+---------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+-----------+-----------+ |
529 | Isolate ID | Gene | Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | | 510 | Isolate ID | Gene | Predicted Phenotype | CGE Predicted Phenotype | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | Sequence | CGE Notes | |
530 +============+============+======================+============+===========+==========================+==============+========+=======+===========+ | 511 +============+========+=====================+===========================+===========+==========+=========================+=============+=======+======+===========+===========+===========+ |
531 | SRR1952908 | sul3 | sulfisoxazole | 100.00 | 100.00 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | | 512 | SRR1952908 | sul3 | sulfisoxazole | Sulfamethoxazole | 100.00 | 100.00 | 792/792 | contig00030 | 2091 | 2882 | AJ459418 | ATGA[...] | | |
532 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | 513 | SRR1952908 | tet(A) | tetracycline | Doxycycline, Tetracycline | 99.92 | 97.80 | 1247/1275 | contig00032 | 1476 | 2722 | AF534183 | ATGT[...] | | |
533 | SRR1952908 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00032 | 1551 | 2750 | AJ517790 | | 514 +------------+--------+---------------------+---------------------------+-----------+----------+-------------------------+-------------+-------+------+-----------+-----------+-----------+ |
534 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | 515 |
535 | SRR1952908 | cmlA1 | chloramphenicol | 99.92 | 100.00 | 1260/1260 | contig00030 | 6707 | 5448 | M64556 | | |
536 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
537 | SRR1952908 | aadA1 | streptomycin | 100.00 | 100.00 | 792/792 | contig00030 | 5355 | 4564 | JQ414041 | | |
538 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
539 | SRR1952908 | aadA2 | streptomycin | 99.75 | 100.00 | 792/792 | contig00030 | 7760 | 6969 | JQ364967 | | |
540 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
541 | SRR1952908 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00032 | 6247 | 5387 | FJ405211 | | |
542 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
543 | SRR1952926 | tet(A) | tetracycline | 99.92 | 100.00 | 1200/1200 | contig00027 | 1480 | 2679 | AJ517790 | | |
544 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
545 | SRR1952926 | blaTEM-57 | ampicillin | 99.88 | 100.00 | 861/861 | contig00027 | 6176 | 5316 | FJ405211 | | |
546 +------------+------------+----------------------+------------+-----------+--------------------------+--------------+--------+-------+-----------+ | |
547 | 516 |
548 plasmidfinder.tsv | 517 plasmidfinder.tsv |
549 ````````````````` | 518 ````````````````` |
550 | 519 |
551 A tabular file of each AMR plasmid type and additional BLAST information from the **PlasmidFinder** database, one plasmid type per line. | 520 A tabular file of each AMR plasmid type and additional BLAST information from the **PlasmidFinder** database, one plasmid type per line. |
552 | 521 |
553 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | 522 +------------+-----------+-----------+----------+-------------------------+-------------+-------+------+-----------+ |
554 | Isolate ID | Plasmid | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | | 523 | Isolate ID | Plasmid | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Accession | |
555 +============+===========+===========+==========+=========================+=============+=======+=======+===========+ | 524 +============+===========+===========+==========+=========================+=============+=======+======+===========+ |
556 | SRR1952908 | ColpVC | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | | 525 | SRR1952908 | ColpVC | 98.96 | 100 | 193/193 | contig00038 | 1618 | 1426 | JX133088 | |
557 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | 526 | SRR1952908 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | |
558 | SRR1952908 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00024 | 10302 | 9660 | FN432031 | | 527 +------------+-----------+-----------+----------+-------------------------+-------------+-------+------+-----------+ |
559 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | 528 |
560 | SRR1952908 | IncFII(S) | 100 | 100 | 262/262 | contig00024 | 54294 | 54555 | CP000858 | | |
561 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
562 | SRR1952908 | IncI1 | 100 | 100 | 142/142 | contig00020 | 3907 | 3766 | AP005147 | | |
563 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
564 | SRR1952926 | ColpVC | 98.96 | 100 | 193/193 | contig00037 | 657 | 849 | JX133088 | | |
565 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
566 | SRR1952926 | IncFIB(S) | 98.91 | 100 | 643/643 | contig00021 | 10302 | 9660 | FN432031 | | |
567 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
568 | SRR1952926 | IncFII(S) | 100 | 100 | 262/262 | contig00021 | 54294 | 54555 | CP000858 | | |
569 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
570 | SRR1952926 | IncI1 | 100 | 100 | 142/142 | contig00017 | 3907 | 3766 | AP005147 | | |
571 +------------+-----------+-----------+----------+-------------------------+-------------+-------+-------+-----------+ | |
572 | 529 |
573 pointfinder.tsv | 530 pointfinder.tsv |
574 ``````````````` | 531 ``````````````` |
575 | 532 |
576 A tabular file of each AMR point mutation and additional BLAST information from the **PointFinder** database, one gene per line. | 533 A tabular file of each AMR point mutation and additional BLAST information from the **PointFinder** database, one gene per line. |
577 | 534 |
578 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ | 535 +------------+-------------+-----------------------------------+------------------------------+-------+----------+---------------------+-----------+----------+-------------------------+-------------+--------+--------+----------------------+-----------+-----------------------+---------------------+------------------+ |
579 | Isolate ID | Gene | Predicted Phenotype | Type | Position | Mutation | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | | 536 | Isolate ID | Gene | Predicted Phenotype | CGE Predicted Phenotype | Type | Position | Mutation | %Identity | %Overlap | HSP Length/Total Length | Contig | Start | End | Pointfinder Position | CGE Notes | CGE Required Mutation | CGE Mechanism | CGE PMID | |
580 +=============+==============+====================================+========+===========+======================+============+===========+==========================+==============+=========+========+ | 537 +============+=============+===================================+==============================+=======+==========+=====================+===========+==========+=========================+=============+========+========+======================+===========+=======================+=====================+==================+ |
581 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00008 | 22801 | 20165 | | 538 | SRR1952908 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | Nalidixic acid,Ciprofloxacin | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00008 | 22801 | 20165 | S83Y | | | Target modification | 7492118,10471553 | |
582 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ | 539 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | Nalidixic acid,Ciprofloxacin | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00011 | 157768 | 160404 | S83Y | | | Target modification | 7492118,10471553 | |
583 | SRR1952926 | gyrA (S83Y) | ciprofloxacin I/R, nalidixic acid | codon | 83 | TCC -> TAC (S -> Y) | 99.96 | 100.00 | 2637/2637 | contig00011 | 157768 | 160404 | | 540 +------------+-------------+-----------------------------------+------------------------------+-------+----------+---------------------+-----------+----------+-------------------------+-------------+--------+--------+----------------------+-----------+-----------------------+---------------------+------------------+ |
584 +-------------+--------------+------------------------------------+--------+-----------+----------------------+------------+-----------+--------------------------+--------------+---------+--------+ | 541 |
585 | 542 |
586 settings.txt | 543 settings.txt |
587 ```````````` | 544 ```````````` |
588 | 545 |
589 The command-line, database versions, and other settings used to run `staramr`. | 546 The command-line, database versions, and other settings used to run `staramr`. |
590 | 547 |
591 :: | 548 :: |
592 | 549 |
593 command_line = staramr search -o out --pointfinder-organism salmonella SRR1952908.fasta SRR1952926.fasta | 550 command_line = staramr search --pointfinder-organism enterococcus_faecium -o out pbp5.fa |
594 version = 0.7.1 | 551 version = 0.10.0 |
595 start_time = 2019-04-25 16:01:32 | 552 start_time = 2023-08-23 13:37:41 |
596 end_time = 2019-04-25 16:01:34 | 553 end_time = 2023-08-23 13:37:42 |
597 total_minutes = 0.04 | 554 total_minutes = 0.02 |
598 resfinder_db_dir = staramr/databases/data/dist/resfinder | 555 resfinder_db_dir = staramr/databases/data/dist/resfinder |
599 resfinder_db_url = https://bitbucket.org/genomicepidemiology/resfinder_db.git | 556 resfinder_db_url = https://bitbucket.org/genomicepidemiology/resfinder_db.git |
600 resfinder_db_commit = e8f1eb2585cd9610c4034a54ce7fc4f93aa95535 | 557 resfinder_db_commit = fa32d9a3cf0c12ec70ca4e90c45c0d590ee810bd |
601 resfinder_db_date = Mon, 16 Jul 2018 16:58 | 558 resfinder_db_date = Tue, 24 May 2022 06:51 |
602 pointfinder_db_dir = staramr/databases/data/dist/pointfinder | 559 pointfinder_db_dir = staramr/databases/data/dist/pointfinder |
603 pointfinder_db_url = https://bitbucket.org/genomicepidemiology/pointfinder_db.git | 560 pointfinder_db_url = https://bitbucket.org/genomicepidemiology/pointfinder_db.git |
604 pointfinder_db_commit = 8706a6363bb29e47e0e398c53043b037c24b99a7 | 561 pointfinder_db_commit = 8c694b9f336153e6d618b897b3b4930961521eb8 |
605 pointfinder_db_date = Wed, 04 Jul 2018 14:27 | 562 pointfinder_db_date = Mon, 01 Feb 2021 15:46 |
563 pointfinder_organisms_all = campylobacter, enterococcus_faecalis, enterococcus_faecium, escherichia_coli, helicobacter_pylori, klebsiella, mycobacterium_tuberculosis, neisseria_gonorrhoeae, plasmodium_falciparum, salmonella, staphylococcus_aureus | |
564 pointfinder_organisms_valid = campylobacter, enterococcus_faecalis, enterococcus_faecium, escherichia_coli, helicobacter_pylori, salmonella | |
606 plasmidfinder_db_dir = staramr/databases/data/dist/plasmidfinder | 565 plasmidfinder_db_dir = staramr/databases/data/dist/plasmidfinder |
607 plasmidfinder_db_url = https://bitbucket.org/genomicepidemiology/plasmidfinder_db.git | 566 plasmidfinder_db_url = https://bitbucket.org/genomicepidemiology/plasmidfinder_db.git |
608 plasmidfinder_db_commit = 81919954cbedaff39056610ab584ab4c06011ed8 | 567 plasmidfinder_db_commit = c18e08c17a5988d4f075fc1171636e47546a323d |
609 plasmidfinder_db_date = Tue, 20 Nov 2018 08:51 | 568 plasmidfinder_db_date = Wed, 18 Jan 2023 09:45 |
610 pointfinder_gene_drug_version = 050218 | 569 mlst_version = 2.23.0 |
611 resfinder_gene_drug_version = 050218.1 | 570 pointfinder_organism = enterococcus_faecium |
571 pointfinder_gene_drug_version = 072621.2 | |
572 resfinder_gene_drug_version = 072621 | |
612 | 573 |
613 results.xlsx | 574 results.xlsx |
614 ```````````` | 575 ```````````` |
615 | 576 |
616 An Excel spreadsheet containing the previous 5 files as separate worksheets. | 577 An Excel spreadsheet containing the previous 5 files as separate worksheets. |
618 BLAST Hits | 579 BLAST Hits |
619 `````````` | 580 `````````` |
620 | 581 |
621 The dataset collection **hits** stores fasta files of the specific blast hits. | 582 The dataset collection **hits** stores fasta files of the specific blast hits. |
622 | 583 |
623 Galaxy wrapper written by Aaron Petkau at the National Microbiology Laboratory, Public Health Agency of Canada. | 584 Galaxy wrapper written by Aaron Petkau and Eric Marinier at the National Microbiology Laboratory, Public Health Agency of Canada. |
624 | 585 |
625 .. _staramr: https://github.com/phac-nml/staramr | 586 .. _staramr: https://github.com/phac-nml/staramr |
626 .. _ResFinder: https://bitbucket.org/genomicepidemiology/resfinder_db | 587 .. _ResFinder: https://bitbucket.org/genomicepidemiology/resfinder_db |
627 .. _PlasmidFinder: https://bitbucket.org/genomicepidemiology/plasmidfinder_db | 588 .. _PlasmidFinder: https://bitbucket.org/genomicepidemiology/plasmidfinder_db |
628 .. _PointFinder: https://bitbucket.org/genomicepidemiology/pointfinder_db | 589 .. _PointFinder: https://bitbucket.org/genomicepidemiology/pointfinder_db |