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date | Fri, 25 Mar 2022 17:35:43 +0000 |
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<tool id="trimal" name="trimAl: a tool for automated alignment trimming" version="0.1.0" python_template_version="3.5"> <requirements> <requirement type="package" version="1.4.1">trimal</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ trimal -in $input1 -out $trimmed_output -htmlout $html_summary ${out_format_selector} #if $trimming_mode.mode_selector == "custom" -gapthreshold $trimming_mode.gapthreshold -simthreshold $trimming_mode.simthreshold -cons $trimming_mode.cons #else: $trimming_mode.mode_selector #end if ; ]]></command> <inputs> <param name="input1" type="data" format="fasta,clustal,pir,phylip,nexus" multiple="false" label="Alignment file (clustal, fasta, NBRF/PIR, nexus, phylip3.2, phylip)" optional="false" /> <conditional name="trimming_mode"> <param name="mode_selector" type="select" label="Select trimming mode from the list"> <option value="-nogaps">nogaps. Remove all positions with gaps in the alignment.</option> <option value="-noallgaps">noallgaps. Remove columns composed only by gaps.</option> <option value="-gappyout">gappyout. Only uses information based on gaps' distribution. (see User Guide)</option> <option value="-strict">strict. (see User Guide)</option> <option value="-strictplus">strictplus. (see User Guide)</option> <option value="custom">Custom mode</option> </param> <when value="custom"> <param argument="-gapthreshold" type="float" optional="true" value="0.9" min="0.0" max="1.0" label="gap threshold" help="1 - (fraction of sequences with a gap allowed). Range: [0 - 1]" multiple="false"/> <param argument="-simthreshold" type="float" optional="true" value="0.9" min="0.0" max="1.0" label="similarity threshold" help="Minimum average similarity allowed. Range: [0 - 1]" multiple="false"/> <param argument="-cons" type="integer" optional="true" value="50" min="0" max="100" label="min conservance percentage" help="Minimum percentage of the positions in the original alignment to conserve." multiple="false"/> </when> </conditional> <param name="out_format_selector" type="select" label="Select trimmed alignment output format from the list"> <option value="-clustal">CLUSTAL format</option> <option value="-fasta">FASTA format</option> <option value="-fasta_m10">FASTA format. Sequences name length up to 10 characters.</option> <option value="-nbrf">NBRF/PIR format</option> <option value="-nexus">NEXUS format</option> <option value="-mega">MEGA format</option> <option value="-phylip">PHYLIP/PHYLIP4 format</option> <option value="-phylip_m10">PHYLIP/PHYLIP4 format. Sequences name length up to 10 characters</option> <option value="-phylip_paml">PHYLIP format compatible with PAML</option> <option value="-phylip_paml_m10">PHYLIP format compatible with PAML. Sequences name length up to 10 characters.</option> <option value="-phylip3.2">PHYLIP3.2 format</option> <option value="-phylip3.2_m10">PHYLIP3.2 format. Sequences name length up to 10 characters.</option> </param> </inputs> <outputs> <data name="trimmed_output" format="fasta" label="Trimmed alignment."/> <data name="html_summary" format="html" label="trimal html summary."/> </outputs> <tests> <test> <param name="input1" value="example.009.AA.fasta"/> <param name="mode_selector" value="-gappyout" /> <param name="out_format_selector" value="-mega" /> <output name="trimmed_output" file="trimmed_example.009.AA.mega" lines_diff="2"/> <output name="html_summary" file="trimmed_example.009.AA.html"/> </test> <test> <param name="input1" value="example.009.AA.fasta"/> <param name="mode_selector" value="custom" /> <param name="gapthreshold" value="0.5" /> <param name="simthreshold" value="0.5" /> <param name="cons" value="5" /> <param name="out_format_selector" value="-phylip_paml_m10" /> <output name="trimmed_output" file="custom_trimmed_example.009.AA.phy" /> <output name="html_summary" file="custom_trimmed_example.009.AA.html"/> </test> </tests> <help><![CDATA[ Modes -nogaps Remove all positions with gaps in the alignment. -noallgaps Remove columns composed only by gaps. -gappyout Use automatic selection on "gappyout" mode. This method only uses information based on gaps' distribution. (see User Guide). -strict Use automatic selection on "strict" mode. (see User Guide). -strictplus Use automatic selection on "strictplus" mode. (see User Guide). trimAl v1.4.rev15 build[2013-12-17]. 2009-2013. Salvador Capella-Gutierrez and Toni Gabaldón. Custom mode parameters -gt -gapthreshold <n> 1 - (fraction of sequences with a gap allowed). -st -simthreshold <n> Minimum average similarity allowed. -cons <n> Minimum percentage of the positions in the original alignment to conserve. Output formats -nbrf Output file in NBRF/PIR format -mega Output file in MEGA format -nexus Output file in NEXUS format -clustal Output file in CLUSTAL format -fasta Output file in FASTA format -fasta_m10 Output file in FASTA format. Sequences name length up to 10 characters. -phylip Output file in PHYLIP/PHYLIP4 format -phylip_m10 Output file in PHYLIP/PHYLIP4 format. Sequences name length up to 10 characters. -phylip_paml Output file in PHYLIP format compatible with PAML -phylip_paml_m10 Output file in PHYLIP format compatible with PAML. Sequences name length up to 10 characters. -phylip3.2 Output file in PHYLIP3.2 format -phylip3.2_m10 Output file in PHYLIP3.2 format. Sequences name length up to 10 characters. trimAl webpage: http://trimal.cgenomics.org This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, the last available version. Please cite: trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Salvador Capella-Gutierrez; Jose M. Silla-Martinez; Toni Gabaldon. Bioinformatics 2009, 25:1972-1973. ]]></help> <citations> <citation type="bibtex"> @misc{githubTrimAl, author = {LastTODO, FirstTODO}, year = {TODO}, title = {TrimAl}, publisher = {GitHub}, journal = {GitHub repository}, url = {https://github.com/inab/trimal}, }</citation> </citations> </tool>