Mercurial > repos > pavlo-lutsik > rnbeads
changeset 12:73e7d00a9a19 draft
Deleted selected files
author | pavlo-lutsik |
---|---|
date | Sun, 21 Apr 2013 18:03:56 -0400 (2013-04-21) |
parents | ce411254e4a9 |
children | d6cdc73fd57e |
files | RnBeadsGalaxy.R automated.settings.xml.txt install.rnbeads.R rnbeads.xml rnbeads_galaxy_wrapper.sh rnbeads_repos.txt.sample tool_data_table_conf.xml.sample tool_dependencies.xml |
diffstat | 8 files changed, 0 insertions(+), 872 deletions(-) [+] |
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line diff
--- a/RnBeadsGalaxy.R Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,173 +0,0 @@ -msg.file<-file("RnBeads.messages.out") -sink(file=open(msg.file, "w")) - -suppressWarnings(suppressPackageStartupMessages(library(RnBeads))) -suppressWarnings(suppressPackageStartupMessages(library(getopt))) - -#all.opts<-names(rnb.options()) -opt.class<-RnBeads:::OPTION.TYPES[-28] -all.opts<-names(opt.class) -#all.opts<-paste("--", all.opts, sep="") -#all.opts<-gsub("\\.([a-z])", "\\U\\1", all.opts, perl=TRUE) -all.opts<-gsub("\\.","-", all.opts) -#opt.class<-sapply(rnb.options(), class) - -rnb.opt.spec<-data.frame( - Long=all.opts, - Short=as.character(1:length(all.opts)), - Mask=c(1,2)[as.integer((opt.class=="logical"))+1], - Type=opt.class) - -### automated xml file preparation -#xml.strings<-apply(rnb.opt.spec,1, function(row){ -# -# opt.lab<-gsub("-", ".", row[1]) -# opt.def.val<-rnb.getOption(opt.lab) -# opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE) -# tf.opt<-"\t\t\t<option value=\"True\">True</option>\n\t\t\t<option value=\"False\">False</option>" -# opt.lab<-paste(opt.lab, gsub("\\."," ", row[4]), sep=", ") -# if(row[4]=="logical"){ -# opt.type<-'select' -# if(!is.null(opt.def.val) && opt.def.val) -# opt.def.val<-"1" else -# opt.def.val<-"0" -# string<-sprintf("\t\t<param name=\"%s\" type=\"%s\" label=\"%s\" value=\"%s\">\n%s\n\t\t</param>\n", opt.name, opt.type, opt.lab, opt.def.val, tf.opt) -# }else{ -# opt.type<-'text' -# if(!is.null(opt.def.val) && opt.def.val!="") -# opt.def.val<-paste(opt.def.val, collapse=",") else -# opt.def.val<-"" -# string<-sprintf("\t\t<param name=\"%s\" type=\"%s\" label=\"%s\" value=\"%s\"/>\n", opt.name, opt.type, opt.lab, opt.def.val) -# } -# string -# }) -# -#cat(xml.strings, sep="", file="automated.settings.xml.txt") -# -#opt.def.strings<-apply(rnb.opt.spec,1, function(row){ -# -# opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE) -# opt.long<-row[1] -# opt.short<-row[2] -# -# if(row[4]=="logical"){ -# def.string<-sprintf("#if str( $options.%s ) == \"True\"\n\t--%s\n#end if\n", opt.name, opt.long) -# }else{ -# def.string<-sprintf("#if str( $options.%s ) != \"\"\n\t--%s=\"$options.%s\" \n#end if\n", opt.name, opt.long, opt.name) -# } -# def.string -# -# }) -#cat(opt.def.strings, sep="", file="automated.option.assignments.txt") - - -rnb.opt.spec$Type<-gsub("\\.vector", "", rnb.opt.spec$Type) -rnb.opt.spec$Type<-gsub("numeric", "double", rnb.opt.spec$Type) -rnb.opt.spec<-rbind(data.frame( - Long=c("data-type", "pheno", "idat-dir","idat-files","bed-files", "gs-report", "geo-series", "betas", "pvals","output-file", "report-dir"), - Short=c("d","s","a","i","f","g","e","b","p","r","o"), - Mask=c(1,2,2,2,2,2,2,2,2,1,1), - Type=c("character","character","character","character","character","character","character","character","character","character", "character")), - rnb.opt.spec) - -opts<-getopt(as.matrix(rnb.opt.spec)) -#opts<-getopt(as.matrix(rnb.opt.spec), opt=list("--data-type=idats","--report-dir=dir", "--idats=file1\tfile2")) -print(opts) - -if(opts[["data-type"]]=="idatDir"){ - - data.source<-list() - data.type<-"idat.dir" - data.source[["idat.dir"]]<-opts[["idat-dir"]] - data.source[["sample.sheet"]]<-opts[["pheno"]] - -}else if(opts[["data-type"]]=="idatFiles"){ - - data.type<-"idat.dir" - files<-strsplit(opts[["idat-files"]], "\t")[[1]] - idat.dir<-mkdir(file.path(opts[["report-dir"]], "idats")) - file.copy(as.list(files), idat.dir) - data.source<-list() - data.source[["idat.dir"]]<-idat.dir - data.source[["sample.sheet"]]<-opts[["pheno"]] - - -}else if(opts[["data-type"]]=="GS.report"){ - - data.type<-"GS.report" - data.source<-opts[["gs-report"]] - -}else if(opts[["data-type"]]=="GEO"){ - - data.type<-"GEO" - data.source<-opts[["geo-series"]] - -}else if(opts[["data-type"]]=="data.files"){ - - data.type<-"GEO" - data.source<-opts[["geo-series"]] - -}else if(opts[["data-type"]]=="data.files"){ - - data.type<-"data.files" - data.source<-c(opts[["pheno"]], opts[["betas"]]) - if(!is.null(opts[["pvals"]])) - data.source<-c(data.source, opts[["pvals"]]) - -}else if(opts[["data-type"]]=="beds"){ - - data.type<-"idat.dir" - files<-strsplit(opts[["bed-files"]], "\t")[[1]] - bed.dir<-mkdir(file.path(opts[["report-dir"]], "beds")) - file.copy(as.list(files), bed.dir) - data.source<-list() - data.source[["bed.dir"]]<-bed.dir - data.source[["sample.sheet"]]<-opts[["pheno"]] - -} - -dump<-sapply(names(opt.class), function(on){ - getoptname<-gsub("-", "\\.","-",on) - if(getoptname %in% names(opts)){ - if(opt.class[on]=="logical"){ - ov<-TRUE - }else if(opt.class %in% c("character","character.vector")){ - ov<-opts[[getoptname]] - ov<-gsub("\"", "", ov) - if(opt.class=="character.vector"){ - ov<-as.character(strsplit(ov,",")) - } - - }else if(opt.class %in% c("integer","numeric","integer.vector","numeric.vector")){ - ov<-opts[[getoptname]] - ov<-gsub("\"", "", ov) - if(opt.class %in% c("integer.vector","numeric.vector")){ - ov<-as.character(strsplit(ov,",")) - } - } - eval(parse(text=sprintf("rnb.options(%s=ov)",on))) - } -}) - - -logical.opts<-names(opt.class[opt.class=="logical"]) -logical.opts.false<-logical.opts[!logical.opts %in% gsub("-",".",names(opts))] - -dump<-sapply(logical.opts.false, function(on){ - eval(parse(text=sprintf("rnb.options(%s=FALSE)",on))) - }) - -#report.out.dir<-sprintf("%s_rnbReport", tempdir()) -report.out.dir<-opts[["report-dir"]] -suppressMessages(suppressWarnings(rnb.run.analysis(data.source=data.source, dir.report=report.out.dir, data.type=data.type))) - -#file.symlink(paste(report.out.dir, "index.html", sep="/"), opts[["output-file"]]) - -outfile<-file(opts[["output-file"]],open="w") -cat(sprintf("<html>\n<body>\n<a href=\"index.html\">RnBeads report</a>\n</body>\n</html>",report.out.dir),file=outfile) -close(outfile) - -sink(file=NULL) -flush(msg.file) -close(msg.file) -
--- a/automated.settings.xml.txt Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,163 +0,0 @@ - <param name="analysisName" type="text" label="analysis.name, character" value=""/> - <param name="logging" type="select" label="logging, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="email" type="text" label="email, character" value=""/> - <param name="assembly" type="text" label="assembly, character" value="hg19"/> - <param name="analyzeSites" type="select" label="analyze.sites, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="regionTypes" type="text" label="region.types, character vector" value="tiling,genes,promoters,cpgislands"/> - <param name="identifiersColumn" type="text" label="identifiers.column, character" value=""/> - <param name="pointsCategory" type="text" label="points.category, integer vector" value="16,17,3,15,4,7,8"/> - <param name="colorsCategory" type="text" label="colors.category, character vector" value="#1B9E77,#D95F02,#7570B3,#E7298A,#66A61E,#E6AB02,#A6761D,#666666"/> - <param name="colorsGradient" type="text" label="colors.gradient, character vector" value="#132B43,#56B1F7"/> - <param name="minGroupSize" type="text" label="min.group.size, integer" value="2"/> - <param name="maxGroupCount" type="text" label="max.group.count, integer" value="8"/> - <param name="gzLargeFiles" type="select" label="gz.large.files, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="strandSpecific" type="select" label="strand.specific, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="replicateIdColumn" type="text" label="replicate.id.column, character" value=""/> - <param name="loadingNormalization" type="select" label="loading.normalization, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="loadingDefaultDataType" type="text" label="loading.default.data.type, character" value="idat.dir"/> - <param name="loadingTableSeparator" type="text" label="loading.table.separator, character" value=" "/> - <param name="loadingBedStyle" type="text" label="loading.bed.style, character" value="BisSNP"/> - <param name="loadingBedColumns" type="text" label="loading.bed.columns, integer vector" value="1,2,3,6,4,5"/> - <param name="loadingBedFrameShift" type="text" label="loading.bed.frame.shift, integer" value="1"/> - <param name="normalizationMethod" type="text" label="normalization.method, character" value="illumina"/> - <param name="normalizationSubtractBackground" type="select" label="normalization.subtract.background, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qc" type="select" label="qc, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcBoxplots" type="select" label="qc.boxplots, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcBarplots" type="select" label="qc.barplots, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcNegativeBoxplot" type="select" label="qc.negative.boxplot, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpHeatmap" type="select" label="qc.snp.heatmap, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpBoxplot" type="select" label="qc.snp.boxplot, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpBarplot" type="select" label="qc.snp.barplot, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSampleBatchSize" type="text" label="qc.sample.batch.size, integer" value="50"/> - <param name="filteringContextRemoval" type="text" label="filtering.context.removal, character vector" value="CC,CAG,CAH,CTG,CTH,Other"/> - <param name="filteringSnp" type="select" label="filtering.snp, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringSnpFrequency" type="text" label="filtering.snp.frequency, double" value="0.01"/> - <param name="filteringSnpAccepted" type="text" label="filtering.snp.accepted, integer" value="2"/> - <param name="filteringSexChromosomesRemoval" type="select" label="filtering.sex.chromosomes.removal, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringMissingValueQuantile" type="text" label="filtering.missing.value.quantile, double" value="1"/> - <param name="filteringCoverageThreshold" type="text" label="filtering.coverage.threshold, integer" value="5"/> - <param name="filteringLowCoverageMasking" type="select" label="filtering.low.coverage.masking, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringHighCoverageOutliers" type="select" label="filtering.high.coverage.outliers, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringGreedycut" type="select" label="filtering.greedycut, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringGreedycutPvalueThreshold" type="text" label="filtering.greedycut.pvalue.threshold, double" value="0.05"/> - <param name="filteringGreedycutRcTies" type="text" label="filtering.greedycut.rc.ties, character" value="row"/> - <param name="filteringDeviationThreshold" type="text" label="filtering.deviation.threshold, double" value="0"/> - <param name="batch" type="select" label="batch, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="batchDreductionColumns" type="text" label="batch.dreduction.columns, integer vector" value=""/> - <param name="batchPrincipalComponents" type="text" label="batch.principal.components, integer" value="8"/> - <param name="batchCorrelationColumns" type="text" label="batch.correlation.columns, integer vector" value=""/> - <param name="batchCorrelationPvalueThreshold" type="text" label="batch.correlation.pvalue.threshold, double" value="0.01"/> - <param name="batchCorrelationPermutations" type="text" label="batch.correlation.permutations, integer" value="10000"/> - <param name="batchCorrelationQc" type="select" label="batch.correlation.qc, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profiles" type="select" label="profiles, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesBetaDistribution" type="select" label="profiles.beta.distribution, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesIntersample" type="select" label="profiles.intersample, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesDeviationPlots" type="select" label="profiles.deviation.plots, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesColumns" type="text" label="profiles.columns, integer vector" value=""/> - <param name="profilesClustering" type="select" label="profiles.clustering, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesClusteringTopProbes" type="text" label="profiles.clustering.top.probes, integer vector" value="1000"/> - <param name="regionProfilesTypes" type="text" label="region.profiles.types, character vector" value="genes,promoters,cpgislands"/> - <param name="differential" type="select" label="differential, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="differentialPermutations" type="text" label="differential.permutations, integer" value="0"/> - <param name="differentialComparisonColumns" type="text" label="differential.comparison.columns, integer vector" value=""/> - <param name="differentialEnrichment" type="select" label="differential.enrichment, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportToUcsc" type="text" label="export.to.ucsc, character vector" value="bigBed,bigWig"/> - <param name="exportToBed" type="select" label="export.to.bed, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportToCsv" type="select" label="export.to.csv, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportTypes" type="text" label="export.types, character vector" value="sites"/> - <param name="colors3Gradient" type="text" label="colors.3.gradient, character vector" value="#832424,#FFFFFF,#3A3A98"/> - <param name="loggingMemory" type="select" label="logging.memory, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="usePstoimg" type="select" label="use.pstoimg, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param>
--- a/install.rnbeads.R Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1 +0,0 @@ -source("http://rnbeads.mpi-inf.mpg.de/install.R") \ No newline at end of file
--- a/rnbeads.xml Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,502 +0,0 @@ -<?xml version="0.9"?> -<tool id="rnbeads" name="RnBeads" version="0.9"> - <description>Performs RnBeads analysis for the selected set of </description> - <requirements> - <requirement type="set_environment" version="0.9">R_SCRIPTS_PATH</requirement> - <requirement type="package" version="3.0.0">R</requirement> - </requirements> - <command interpreter="bash">rnbeads_galaxy_wrapper.sh - #if str( $inputDataSelector.dataType ) == "idat" - --data-type="idat.dir" - --pheno="$inputDataSelector.sampleAnnotations" - #if str( $idatSelector.idatSelector.idatSource ) == "history" - --idat-files ==" - #for $input_files in $inputDataSelector.input_series: - ${input_files.input_files}\t - #end for - " - #else: - --idat-files == "$idatRepo" - #end if - #end if - #if str( $inputDataSelector.dataType ) == "gsreport" - --data-type="GS.report" - --gs-report="$inputDataSelector.gsReportFile" - #end if - #if str( $inputDataSelector.dataType ) == "geo" - --data-type="GEO" - --geo-series=$inputDataSelector.geoSeries - #end if - #if str( $inputDataSelector.dataType ) == "tables" - --data-type="data.files" - --pheno="$inputDataSelector.sampleAnnotations" - #end if - #if str( $inputDataSelector.dataType ) == "tables" - --data-type="data.files" - --pheno="$inputDataSelector.sampleAnnotations" - --betas="$inputDataSelector.betaTable" - --pvals="$inputDataSelector.pvalTable" - #end if - #if str( $inputDataSelector.dataType ) == "bed" - --data-type="bed.dir" - --pheno="$inputDataSelector.sampleAnnotations" - --beds ==" - #for $input_files in $inputDataSelector.input_series: - ${input_files.input_files}\t - #end for - #end if - --report-dir="$html_file.extra_files_path" - --output-file="$html_file" - #if str( $options.optionSet ) == "full" - #if str( $options.analysisName ) != "" - --analysis-name="$options.analysisName" - #end if - #if str( $options.logging ) == "True" - --logging - #end if - #if str( $options.email ) != "" - --email="$options.email" - #end if - #if str( $options.assembly ) != "" - --assembly="$options.assembly" - #end if - #if str( $options.analyzeSites ) == "True" - --analyze-sites - #end if - #if str( $options.regionTypes ) != "" - --region-types="$options.regionTypes" - #end if - #if str( $options.identifiersColumn ) != "" - --identifiers-column="$options.identifiersColumn" - #end if - #if str( $options.pointsCategory ) != "" - --points-category="$options.pointsCategory" - #end if - #if str( $options.colorsCategory ) != "" - --colors-category="$options.colorsCategory" - #end if - #if str( $options.colorsGradient ) != "" - --colors-gradient="$options.colorsGradient" - #end if - #if str( $options.minGroupSize ) != "" - --min-group-size="$options.minGroupSize" - #end if - #if str( $options.maxGroupCount ) != "" - --max-group-count="$options.maxGroupCount" - #end if - #if str( $options.gzLargeFiles ) == "True" - --gz-large-files - #end if - #if str( $options.strandSpecific ) == "True" - --strand-specific - #end if - #if str( $options.replicateIdColumn ) != "" - --replicate-id-column="$options.replicateIdColumn" - #end if - #if str( $options.loadingNormalization ) == "True" - --loading-normalization - #end if - #if str( $options.loadingDefaultDataType ) != "" - --loading-default-data-type="$options.loadingDefaultDataType" - #end if - #if str( $options.loadingTableSeparator ) != "" - --loading-table-separator="$options.loadingTableSeparator" - #end if - #if str( $options.loadingBedStyle ) != "" - --loading-bed-style="$options.loadingBedStyle" - #end if - #if str( $options.loadingBedColumns ) != "" - --loading-bed-columns="$options.loadingBedColumns" - #end if - #if str( $options.loadingBedFrameShift ) != "" - --loading-bed-frame-shift="$options.loadingBedFrameShift" - #end if - #if str( $options.normalizationMethod ) != "" - --normalization-method="$options.normalizationMethod" - #end if - #if str( $options.normalizationSubtractBackground ) == "True" - --normalization-subtract-background - #end if - #if str( $options.qc ) == "True" - --qc - #end if - #if str( $options.qcBoxplots ) == "True" - --qc-boxplots - #end if - #if str( $options.qcBarplots ) == "True" - --qc-barplots - #end if - #if str( $options.qcNegativeBoxplot ) == "True" - --qc-negative-boxplot - #end if - #if str( $options.qcSnpHeatmap ) == "True" - --qc-snp-heatmap - #end if - #if str( $options.qcSnpBoxplot ) == "True" - --qc-snp-boxplot - #end if - #if str( $options.qcSnpBarplot ) == "True" - --qc-snp-barplot - #end if - #if str( $options.qcSampleBatchSize ) != "" - --qc-sample-batch-size="$options.qcSampleBatchSize" - #end if - #if str( $options.filteringContextRemoval ) != "" - --filtering-context-removal="$options.filteringContextRemoval" - #end if - #if str( $options.filteringSnp ) == "True" - --filtering-snp - #end if - #if str( $options.filteringSnpFrequency ) != "" - --filtering-snp-frequency="$options.filteringSnpFrequency" - #end if - #if str( $options.filteringSnpAccepted ) != "" - --filtering-snp-accepted="$options.filteringSnpAccepted" - #end if - #if str( $options.filteringSexChromosomesRemoval ) == "True" - --filtering-sex-chromosomes-removal - #end if - #if str( $options.filteringMissingValueQuantile ) != "" - --filtering-missing-value-quantile="$options.filteringMissingValueQuantile" - #end if - #if str( $options.filteringCoverageThreshold ) != "" - --filtering-coverage-threshold="$options.filteringCoverageThreshold" - #end if - #if str( $options.filteringLowCoverageMasking ) == "True" - --filtering-low-coverage-masking - #end if - #if str( $options.filteringHighCoverageOutliers ) == "True" - --filtering-high-coverage-outliers - #end if - #if str( $options.filteringGreedycut ) == "True" - --filtering-greedycut - #end if - #if str( $options.filteringGreedycutPvalueThreshold ) != "" - --filtering-greedycut-pvalue-threshold="$options.filteringGreedycutPvalueThreshold" - #end if - #if str( $options.filteringGreedycutRcTies ) != "" - --filtering-greedycut-rc-ties="$options.filteringGreedycutRcTies" - #end if - #if str( $options.filteringDeviationThreshold ) != "" - --filtering-deviation-threshold="$options.filteringDeviationThreshold" - #end if - #if str( $options.batch ) == "True" - --batch - #end if - #if str( $options.batchDreductionColumns ) != "" - --batch-dreduction-columns="$options.batchDreductionColumns" - #end if - #if str( $options.batchPrincipalComponents ) != "" - --batch-principal-components="$options.batchPrincipalComponents" - #end if - #if str( $options.batchCorrelationColumns ) != "" - --batch-correlation-columns="$options.batchCorrelationColumns" - #end if - #if str( $options.batchCorrelationPvalueThreshold ) != "" - --batch-correlation-pvalue-threshold="$options.batchCorrelationPvalueThreshold" - #end if - #if str( $options.batchCorrelationPermutations ) != "" - --batch-correlation-permutations="$options.batchCorrelationPermutations" - #end if - #if str( $options.batchCorrelationQc ) == "True" - --batch-correlation-qc - #end if - #if str( $options.profiles ) == "True" - --profiles - #end if - #if str( $options.profilesBetaDistribution ) == "True" - --profiles-beta-distribution - #end if - #if str( $options.profilesIntersample ) == "True" - --profiles-intersample - #end if - #if str( $options.profilesDeviationPlots ) == "True" - --profiles-deviation-plots - #end if - #if str( $options.profilesColumns ) != "" - --profiles-columns="$options.profilesColumns" - #end if - #if str( $options.profilesClustering ) == "True" - --profiles-clustering - #end if - #if str( $options.profilesClusteringTopProbes ) != "" - --profiles-clustering-top-probes="$options.profilesClusteringTopProbes" - #end if - #if str( $options.regionProfilesTypes ) != "" - --region-profiles-types="$options.regionProfilesTypes" - #end if - #if str( $options.differential ) == "True" - --differential - #end if - #if str( $options.differentialPermutations ) != "" - --differential-permutations="$options.differentialPermutations" - #end if - #if str( $options.differentialComparisonColumns ) != "" - --differential-comparison-columns="$options.differentialComparisonColumns" - #end if - #if str( $options.differentialEnrichment ) == "True" - --differential-enrichment - #end if - #if str( $options.exportToUcsc ) != "" - --export-to-ucsc="$options.exportToUcsc" - #end if - #if str( $options.exportToBed ) == "True" - --export-to-bed - #end if - #if str( $options.exportToCsv ) == "True" - --export-to-csv - #end if - #if str( $options.exportTypes ) != "" - --export-types="$options.exportTypes" - #end if - #if str( $options.colors3Gradient ) != "" - --colors-3-gradient="$options.colors3Gradient" - #end if - #if str( $options.loggingMemory ) == "True" - --logging-memory - #end if - #if str( $options.usePstoimg ) == "True" - --use-pstoimg - #end if - #end if - </command> - <inputs> - <conditional name="inputDataSelector"> - <param name="dataType" type="select" label="Data Type"> - <option value="tables">Tabular data</option> - <option value="idats">IDAT files</option> - <option value="gsreport">GenomeStudio report</option> - <option value="geo">Gene Expression Omnibus series</option> - <option value="bed">BED files</option> - </param> - <when value="tables"> - <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/> - <param name="betaTable" type="data" format="tabular" label="Table with beta-values"/> - <param name="pvalTable" type="data" format="tabular" label="Table with detection p\-values"/> - </when> - <when value="idats"> - <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/> - <conditional name="idatSelector"> - <param name="idatSource" type="select" label="IDAT source"> - <option value="history">Select from history</option> - <option value="repo">Repository on the server</option> - </param> - <when value="repo"> - <param name="idatRepo" type="select" label="Select an IDAT repository"> - <options from_data_table="rnbeads_repos"> - <filter type="sort_by" column="2" /> - <validator type="no_options" message="No repositories are available" /> - </options> - </param> - </when> - <when value="history"> - <repeat min="1" name="idatSeries" title="Input IDAT file"> - <param name="idatFiles" type="data" format="idat" label="IDAT file"/> - </repeat> - </when> - </conditional> - </when> - <when value="gsreport"> - <param name="gsReportFile" type="data" format="tabular" label="GenomeStudio report" /> - </when> - <when value="geo"> - <param name="geoSeries" type="text" label="GEO series" /> - </when> - <when value="bed"> - <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File" /> - <repeat min="1" name="bedSeries" title="Input BED file"> - <param name="idatFiles" type="data" format="idat"/> - </repeat> - </when> - </conditional> - <conditional name="options"> - <param name="optionSet" type="select" label="Options Set"> - <option value="default">Default options</option> - <option value="full">Full option set</option> - </param> - <when value="default" /> - <when value="full"> - <param name="analysisName" type="text" label="analysis.name, character" value=""/> - <param name="logging" type="select" label="logging, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="email" type="text" label="email, character" value=""/> - <param name="assembly" type="text" label="assembly, character" value="hg19"/> - <param name="analyzeSites" type="select" label="analyze.sites, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="regionTypes" type="text" label="region.types, character vector" value="tiling,genes,promoters,cpgislands"/> - <param name="identifiersColumn" type="text" label="identifiers.column, character" value=""/> - <param name="pointsCategory" type="text" label="points.category, integer vector" value="16,17,3,15,4,7,8"/> - <param name="colorsCategory" type="text" label="colors.category, character vector" value="#1B9E77,#D95F02,#7570B3,#E7298A,#66A61E,#E6AB02,#A6761D,#666666"/> - <param name="colorsGradient" type="text" label="colors.gradient, character vector" value="#132B43,#56B1F7"/> - <param name="minGroupSize" type="text" label="min.group.size, integer" value="2"/> - <param name="maxGroupCount" type="text" label="max.group.count, integer" value="8"/> - <param name="gzLargeFiles" type="select" label="gz.large.files, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="strandSpecific" type="select" label="strand.specific, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="replicateIdColumn" type="text" label="replicate.id.column, character" value=""/> - <param name="loadingNormalization" type="select" label="loading.normalization, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="loadingDefaultDataType" type="text" label="loading.default.data.type, character" value="idat.dir"/> - <param name="loadingTableSeparator" type="text" label="loading.table.separator, character" value=","/> - <param name="loadingBedStyle" type="text" label="loading.bed.style, character" value="BisSNP"/> - <param name="loadingBedColumns" type="text" label="loading.bed.columns, integer vector" value="1,2,3,6,4,5"/> - <param name="loadingBedFrameShift" type="text" label="loading.bed.frame.shift, integer" value="1"/> - <param name="normalizationMethod" type="text" label="normalization.method, character" value="illumina"/> - <param name="normalizationSubtractBackground" type="select" label="normalization.subtract.background, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qc" type="select" label="qc, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcBoxplots" type="select" label="qc.boxplots, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcBarplots" type="select" label="qc.barplots, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcNegativeBoxplot" type="select" label="qc.negative.boxplot, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpHeatmap" type="select" label="qc.snp.heatmap, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpBoxplot" type="select" label="qc.snp.boxplot, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSnpBarplot" type="select" label="qc.snp.barplot, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="qcSampleBatchSize" type="text" label="qc.sample.batch.size, integer" value="50"/> - <param name="filteringContextRemoval" type="text" label="filtering.context.removal, character vector" value="CC,CAG,CAH,CTG,CTH,Other"/> - <param name="filteringSnp" type="select" label="filtering.snp, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringSnpFrequency" type="text" label="filtering.snp.frequency, double" value="0.01"/> - <param name="filteringSnpAccepted" type="text" label="filtering.snp.accepted, integer" value="2"/> - <param name="filteringSexChromosomesRemoval" type="select" label="filtering.sex.chromosomes.removal, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringMissingValueQuantile" type="text" label="filtering.missing.value.quantile, double" value="1"/> - <param name="filteringCoverageThreshold" type="text" label="filtering.coverage.threshold, integer" value="5"/> - <param name="filteringLowCoverageMasking" type="select" label="filtering.low.coverage.masking, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringHighCoverageOutliers" type="select" label="filtering.high.coverage.outliers, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringGreedycut" type="select" label="filtering.greedycut, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="filteringGreedycutPvalueThreshold" type="text" label="filtering.greedycut.pvalue.threshold, double" value="0.05"/> - <param name="filteringGreedycutRcTies" type="text" label="filtering.greedycut.rc.ties, character" value="row"/> - <param name="filteringDeviationThreshold" type="text" label="filtering.deviation.threshold, double" value="0"/> - <param name="batch" type="select" label="batch, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="batchDreductionColumns" type="text" label="batch.dreduction.columns, integer vector" value=""/> - <param name="batchPrincipalComponents" type="text" label="batch.principal.components, integer" value="8"/> - <param name="batchCorrelationColumns" type="text" label="batch.correlation.columns, integer vector" value=""/> - <param name="batchCorrelationPvalueThreshold" type="text" label="batch.correlation.pvalue.threshold, double" value="0.01"/> - <param name="batchCorrelationPermutations" type="text" label="batch.correlation.permutations, integer" value="10000"/> - <param name="batchCorrelationQc" type="select" label="batch.correlation.qc, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profiles" type="select" label="profiles, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesBetaDistribution" type="select" label="profiles.beta.distribution, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesIntersample" type="select" label="profiles.intersample, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesDeviationPlots" type="select" label="profiles.deviation.plots, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesColumns" type="text" label="profiles.columns, integer vector" value=""/> - <param name="profilesClustering" type="select" label="profiles.clustering, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="profilesClusteringTopProbes" type="text" label="profiles.clustering.top.probes, integer vector" value="1000"/> - <param name="regionProfilesTypes" type="text" label="region.profiles.types, character vector" value="genes,promoters,cpgislands"/> - <param name="differential" type="select" label="differential, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="differentialPermutations" type="text" label="differential.permutations, integer" value="0"/> - <param name="differentialComparisonColumns" type="text" label="differential.comparison.columns, integer vector" value=""/> - <param name="differentialEnrichment" type="select" label="differential.enrichment, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportToUcsc" type="text" label="export.to.ucsc, character vector" value="bigBed,bigWig"/> - <param name="exportToBed" type="select" label="export.to.bed, logical" value="1"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportToCsv" type="select" label="export.to.csv, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="exportTypes" type="text" label="export.types, character vector" value="sites"/> - <param name="colors3Gradient" type="text" label="colors.3.gradient, character vector" value="#832424,#FFFFFF,#3A3A98"/> - <param name="loggingMemory" type="select" label="logging.memory, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - <param name="usePstoimg" type="select" label="use.pstoimg, logical" value="0"> - <option value="True">True</option> - <option value="False">False</option> - </param> - </when> - </conditional> - </inputs> - - <outputs> - <data format="html" name="html_file" label="index.html" /> - </outputs> - - <!-- - <tests> - <test> - <param name="input" value="fa_gc_content_input.fa"/> - <output name="out_file1" file="fa_gc_content_output.txt"/> - </test> - </tests> --> - - <help> - RnBeads is an R-package for comprehensive analysis of Illumina Infinium HumanMethylation450 BeadChip data. It extends previous approaches for this type of data analysis by high throughput capabilities, as well as presenting results in a comprehensive, highly interpretable fashion. - </help> - -</tool> \ No newline at end of file
--- a/rnbeads_galaxy_wrapper.sh Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ -#!/bin/bash -Rscript --no-save $(dirname $(readlink -f $0))/RnBeadsGalaxy.R $* > /dev/null 2>stderr.out -#Rscript --no-save \$R_SCRIPTS_PATH/RnBeadsGalaxy.R $* -exit 0 \ No newline at end of file
--- a/rnbeads_repos.txt.sample Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1 +0,0 @@ -repo1 Repository1 /data/iscan/idats \ No newline at end of file
--- a/tool_data_table_conf.xml.sample Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,6 +0,0 @@ -<tables> - <table name="rnbeads_repos" comment_char="#"> - <columns>id, name, value</columns> - <file path="tool-data/rnbeads_repos.txt" /> - </table> -</tables> \ No newline at end of file
--- a/tool_dependencies.xml Sat Apr 20 07:14:22 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,22 +0,0 @@ -<?xml version="1.0"?> -<tool_dependency> - <set_environment version="0.9"> - <environment_variable name="R_SCRIPTS_PATH" action="set_to">$REPOSITORY_INSTALL_DIR</environment_variable> - </set_environment> - <package name="R" version="3.0.0"> - <install version="0.9"> - <actions> - <action type="download_by_url">http://CRAN.R-project.org/src/base/R-3/R-3.0.0.tar.gz</action> - <action type="shell_command">./configure --prefix=$INSTALL_DIR --with-x=no</action> - <action type="shell_command">make</action> - <action type="set_environment"> - <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable> - </action> - <action type="shell_command">R --no-save <<< "install.packages("getopt"); source('http://rnbeads.mpi-inf.mpg.de')"</action> - </actions> - </install> - <readme> - You need a FORTRAN compiler or perhaps f2c in addition to a C compiler to build R. - </readme> - </package> -</tool_dependency> \ No newline at end of file