Mercurial > repos > peterjc > fastq_filter_by_id
diff tools/fastq/fastq_filter_by_id.xml @ 3:e0041942a12d draft default tip
v0.0.5 - galaxy_sequence_utils dependency and other cleanups inc using MIT license
author | peterjc |
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date | Fri, 03 Feb 2017 05:34:18 -0500 |
parents | d570cc324779 |
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--- a/tools/fastq/fastq_filter_by_id.xml Tue Jun 07 17:24:08 2011 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,81 +0,0 @@ -<tool id="fastq_filter_by_id" name="Filter FASTQ by ID" version="0.0.4" hidden="true"> - <description>from a tabular file</description> - <command interpreter="python"> -fastq_filter_by_id.py $input_tabular $columns $input_fastq -#if $output_choice_cond.output_choice=="both" - $output_pos $output_neg -#elif $output_choice_cond.output_choice=="pos" - $output_pos - -#elif $output_choice_cond.output_choice=="neg" - - $output_neg -#end if - </command> - <inputs> - <param name="input_fastq" type="data" format="fastq" label="FASTQ file to filter on the identifiers"/> - <param name="input_tabular" type="data" format="tabular" label="Tabular file containing FASTQ identifiers"/> - <param name="columns" type="data_column" data_ref="input_tabular" multiple="True" numerical="False" label="Column(s) containing FASTA identifiers" help="Multi-select list - hold the appropriate key while clicking to select multiple columns"> - <validator type="no_options" message="Pick at least one column"/> - </param> - <conditional name="output_choice_cond"> - <param name="output_choice" type="select" label="Output positive matches, negative matches, or both?"> - <option value="both">Both positive matches (ID on list) and negative matches (ID not on list), as two FASTA files</option> - <option value="pos">Just positive matches (ID on list), as a single FASTA file</option> - <option value="neg">Just negative matches (ID not on list), as a single FASTA file</option> - </param> - <!-- Seems need these dummy entries here, compare this to indels/indel_sam2interval.xml --> - <when value="both" /> - <when value="pos" /> - <when value="neg" /> - </conditional> - </inputs> - <outputs> - <data name="output_pos" format="fastq" label="With matched ID"> - <!-- TODO - Replace this with format="input:input_fastq" if/when that works --> - <change_format> - <when input_dataset="input_fastq" attribute="extension" value="fastqsanger" format="fastqsanger" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqsolexa" format="fastqsolexa" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqillumina" format="fastqillumina" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqcssanger" format="fastqcssanger" /> - </change_format> - <filter>output_choice_cond["output_choice"] != "neg"</filter> - </data> - <data name="output_neg" format="fastq" label="Without matched ID"> - <!-- TODO - Replace this with format="input:input_fastq" if/when that works --> - <change_format> - <when input_dataset="input_fastq" attribute="extension" value="fastqsanger" format="fastqsanger" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqsolexa" format="fastqsolexa" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqillumina" format="fastqillumina" /> - <when input_dataset="input_fastq" attribute="extension" value="fastqcssanger" format="fastqcssanger" /> - </change_format> - <filter>output_choice_cond["output_choice"] != "pos"</filter> - </data> - </outputs> - <tests> - </tests> - <help> - -**Deprecated** - -This tool is now obsolete, and should not be used in future. It has been -replaced by a more general version covering FASTA, FASTQ and SFF in one -single tool. - -**What it does** - -By default it divides a FASTQ file in two, those sequences with or without an -ID present in the tabular file column(s) specified. You can opt to have a -single output file of just the matching records, or just the non-matching ones. - -Note that the order of sequences in the original FASTA file is preserved. -Also, if any sequences share an identifier, duplicates are not removed. - -**Example Usage** - -You may have performed some kind of contamination search, for example running -BLASTN against a database of cloning vectors or bacteria, giving you a tabular -file containing read identifiers. You could use this tool to extract only the -reads without BLAST matches (i.e. those which do not match your contaminant -database). - - </help> -</tool>