Mercurial > repos > peterjc > fastq_paired_unpaired
diff tools/fastq/fastq_paired_unpaired.xml @ 1:7ed81e36fc1c
Uploaded v0.0.5 which handles Illumina 1.8 style pair naming.
author | peterjc |
---|---|
date | Mon, 12 Dec 2011 11:33:10 -0500 |
parents | 72e9fcaec61f |
children | 95a632a71951 |
line wrap: on
line diff
--- a/tools/fastq/fastq_paired_unpaired.xml Tue Jun 07 17:21:17 2011 -0400 +++ b/tools/fastq/fastq_paired_unpaired.xml Mon Dec 12 11:33:10 2011 -0500 @@ -1,4 +1,4 @@ -<tool id="fastq_paired_unpaired" name="Divide FASTQ file into paired and unpaired reads" version="0.0.4"> +<tool id="fastq_paired_unpaired" name="Divide FASTQ file into paired and unpaired reads" version="0.0.5"> <description>using the read name suffices</description> <command interpreter="python"> fastq_paired_unpaired.py $input_fastq.extension $input_fastq @@ -52,29 +52,34 @@ Any reads where the forward/reverse naming suffix used is not recognised are treated as orphan reads. The tool supports the /1 and /2 convention -used by Illumina, the .f and .r convention, and the Sanger convention -(see http://staden.sourceforge.net/manual/pregap4_unix_50.html for details). +originally used by Illumina, .f and .r convention, the Sanger convention +(see http://staden.sourceforge.net/manual/pregap4_unix_50.html for details), +and the current Illumina convention where the reads get the same identifier +with the fragment number in the description, for example: + + * @HWI-ST916:79:D04M5ACXX:1:1101:10000:100326 1:N:0:TGNCCA + * @HWI-ST916:79:D04M5ACXX:1:1101:10000:100326 2:N:0:TGNCCA Note that this does support multiple forward and reverse reads per template (which is quite common with Sanger sequencing), e.g. this which is sorted alphabetically: -WTSI_1055_4p17.p1kapIBF -WTSI_1055_4p17.p1kpIBF -WTSI_1055_4p17.q1kapIBR -WTSI_1055_4p17.q1kpIBR + * WTSI_1055_4p17.p1kapIBF + * WTSI_1055_4p17.p1kpIBF + * WTSI_1055_4p17.q1kapIBR + * WTSI_1055_4p17.q1kpIBR or this where the reads already come in pairs: -WTSI_1055_4p17.p1kapIBF -WTSI_1055_4p17.q1kapIBR -WTSI_1055_4p17.p1kpIBF -WTSI_1055_4p17.q1kpIBR + * WTSI_1055_4p17.p1kapIBF + * WTSI_1055_4p17.q1kapIBR + * WTSI_1055_4p17.p1kpIBF + * WTSI_1055_4p17.q1kpIBR both become: -WTSI_1055_4p17.p1kapIBF paired with WTSI_1055_4p17.q1kapIBR -WTSI_1055_4p17.p1kpIBF paired with WTSI_1055_4p17.q1kpIBR + * WTSI_1055_4p17.p1kapIBF paired with WTSI_1055_4p17.q1kapIBR + * WTSI_1055_4p17.p1kpIBF paired with WTSI_1055_4p17.q1kpIBR </help> </tool>