# HG changeset patch # User peterjc # Date 1431527750 14400 # Node ID 8945bad80f4af0f5f7711666c7651e2b69147274 # Parent d4412c04d7b13f33f543283dd168d5d64c65534b v0.0.4; internal changes for packaging diff -r d4412c04d7b1 -r 8945bad80f4a tools/samtools_idxstats/README.rst --- a/tools/samtools_idxstats/README.rst Wed Nov 20 12:27:33 2013 -0500 +++ b/tools/samtools_idxstats/README.rst Wed May 13 10:35:50 2015 -0400 @@ -1,7 +1,7 @@ Galaxy wrapper for samtools idxstats ==================================== -This wrapper is copyright 2013 by Peter Cock, The James Hutton Institute +This wrapper is copyright 2013-2015 by Peter Cock, The James Hutton Institute (formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. See the licence text below. @@ -26,19 +26,19 @@ To install the wrapper copy or move the following files under the Galaxy tools folder, e.g. in a ``tools/samtools_idxstats`` folder: -* samtools_idxstats.xml (the Galaxy tool definition) -* samtools_idxstats.py (the Python wrapper script) -* README.rst (this file) +* ``samtools_idxstats.xml`` (the Galaxy tool definition) +* ``samtools_idxstats.py`` (the Python wrapper script) +* ``README.rst`` (this file) You will also need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool. Just add the line, perhaps under the NGS tools section:: -If you wish to run the unit tests, also add this to tools_conf.xml.sample -and move/copy the test-data files under Galaxy's test-data folder. Then:: +If you wish to run the unit tests, also move/copy the ``test-data/`` files +under Galaxy's ``test-data/`` folder. Then:: - $ ./run_functional_tests.sh -id samtools_idxstats + $ ./run_tests.sh -id samtools_idxstats That's it. @@ -50,29 +50,43 @@ Version Changes ------- ---------------------------------------------------------------------- v0.0.1 - Initial public release +v0.0.2 - Use quoted filenames when calling samtools (in case of spaces etc) +v0.0.3 - Embed samtools citation in tool XML. +v0.0.4 - Reorder XML elements (internal change only). + - Planemo for Tool Shed upload (``.shed.yml``, internal change only). ======= ====================================================================== Developers ========== -Development is one this GitHub repository: +Development is on this GitHub repository: https://github.com/peterjc/pico_galaxy/tree/master/tools/samtools_idxstats -For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use -the following command from the Galaxy root folder:: - $ tar -czf samtools_idxstats.tar.gz tools/samtools_idxstats/README.rst tools/samtools_idxstats/samtools_idxstats.xml tools/samtools_idxstats/samtools_idxstats.py tools/samtools_idxstats/tool_dependencies.xml test-data/ex1.bam test-data/ex1.idxstats.tabular +For pushing a release to the test or main "Galaxy Tool Shed", use the following +Planemo commands (which requires you have set your Tool Shed access details in +``~/.planemo.yml`` and that you have access rights on the Tool Shed):: + + $ planemo shed_upload --shed_target testtoolshed --check_diff ~/repositories/pico_galaxy/tools/samtools_idxstats/ + ... -Check this worked:: +or:: + + $ planemo shed_upload --shed_target toolshed --check_diff ~/repositories/pico_galaxy/tools/samtools_idxstats/ + ... - $ tar -tzf samtools_idxstats.tar.gz - tools/samtools_idxstats/README.rst - tools/samtools_idxstats/samtools_idxstats.xml - tools/samtools_idxstats/samtools_idxstats.py - tools/samtools_idxstats/tool_dependencies.xml +To just build and check the tar ball, use:: + + $ planemo shed_upload --tar_only ~/repositories/pico_galaxy/tools/samtools_idxstats/ + ... + $ tar -tzf shed_upload.tar.gz test-data/ex1.bam test-data/ex1.idxstats.tabular + tools/samtools_idxstats/README.rst + tools/samtools_idxstats/samtools_idxstats.py + tools/samtools_idxstats/samtools_idxstats.xml + tools/samtools_idxstats/tool_dependencies.xml Licence (MIT) diff -r d4412c04d7b1 -r 8945bad80f4a tools/samtools_idxstats/samtools_idxstats.py --- a/tools/samtools_idxstats/samtools_idxstats.py Wed Nov 20 12:27:33 2013 -0500 +++ b/tools/samtools_idxstats/samtools_idxstats.py Wed May 13 10:35:50 2015 -0400 @@ -17,26 +17,26 @@ if "-v" in sys.argv or "--version" in sys.argv: #Galaxy seems to invert the order of the two lines - print "(Galaxy wrapper v0.0.1)" + print "(Galaxy wrapper v0.0.2)" cmd = "samtools 2>&1 | grep -i ^Version" sys.exit(os.system(cmd)) -def stop_err(msg, error_level=1): +def sys_exit(msg, error_level=1): """Print error message to stdout and quit with given error level.""" sys.stderr.write("%s\n" % msg) sys.exit(error_level) if len(sys.argv) != 4: - stop_err("Require three arguments: BAM, BAI, tabular filenames") + sys_exit("Require three arguments: BAM, BAI, tabular filenames") bam_filename, bai_filename, tabular_filename = sys.argv[1:] if not os.path.isfile(bam_filename): - stop_err("Input BAM file not found: %s" % bam_filename) + sys_exit("Input BAM file not found: %s" % bam_filename) if not os.path.isfile(bai_filename): if bai_filename == "None": - stop_err("Error: Galaxy did not index your BAM file") - stop_err("Input BAI file not found: %s" % bai_filename) + sys_exit("Error: Galaxy did not index your BAM file") + sys_exit("Input BAI file not found: %s" % bai_filename) #Assign sensible names with real extensions, and setup symlinks: tmp_dir = tempfile.mkdtemp() @@ -49,7 +49,7 @@ assert os.path.isfile(bam_file + ".bai"), bam_file #Run samtools idxstats: -cmd = "samtools idxstats %s > %s" % (bam_file, tabular_filename) +cmd = 'samtools idxstats "%s" > "%s"' % (bam_file, tabular_filename) return_code = os.system(cmd) #Remove the temp symlinks: @@ -58,4 +58,4 @@ os.rmdir(tmp_dir) if return_code: - stop_err("Return code %i from command:\n%s" % (return_code, cmd)) + sys_exit("Return code %i from command:\n%s" % (return_code, cmd)) diff -r d4412c04d7b1 -r 8945bad80f4a tools/samtools_idxstats/samtools_idxstats.xml --- a/tools/samtools_idxstats/samtools_idxstats.xml Wed Nov 20 12:27:33 2013 -0500 +++ b/tools/samtools_idxstats/samtools_idxstats.xml Wed May 13 10:35:50 2015 -0400 @@ -1,9 +1,14 @@ - + samtools idxstats samtools samtools + + + + + samtools_idxstats.py --version samtools_idxstats.py "$input_bam" "${input_bam.metadata.bam_index}" "$out_tabular" @@ -12,11 +17,6 @@ - - - - - @@ -73,4 +73,7 @@ This wrapper is available to install into other Galaxy Instances via the Galaxy Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/samtools_idxstats + + 10.1093/bioinformatics/btp352 + diff -r d4412c04d7b1 -r 8945bad80f4a tools/samtools_idxstats/tool_dependencies.xml --- a/tools/samtools_idxstats/tool_dependencies.xml Wed Nov 20 12:27:33 2013 -0500 +++ b/tools/samtools_idxstats/tool_dependencies.xml Wed May 13 10:35:50 2015 -0400 @@ -1,6 +1,6 @@ - +