Mercurial > repos > peterjc > seq_primer_clip
diff tools/seq_primer_clip/README.rst @ 2:ee5acea162a7 draft
Uploaded v0.0.10, README now using RST, MIT licence, automatic Biopython dependency
author | peterjc |
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date | Thu, 24 Oct 2013 09:37:25 -0400 |
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children | 708ce1e5ac94 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/seq_primer_clip/README.rst Thu Oct 24 09:37:25 2013 -0400 @@ -0,0 +1,120 @@ +Galaxy tool to primer clip (trim) FASTA, FASTQ or SFF reads +=========================================================== + +This tool is copyright 2011-2013 by Peter Cock, The James Hutton Institute +(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. +See the licence text below (MIT licence). + +This tool is a short Python script (using the Galaxy library functions and +Biopython). It is available from the Galaxy Tool Shed here: +http://toolshed.g2.bx.psu.edu/view/peterjc/seq_primer_clip + + +Automated Installation +====================== + +This should be straightforward using the Galaxy Tool Shed, which should be +able to automatically install the dependency on Biopython, and then install +this tool and run its unit tests. + + +Manual Installation +=================== + +There are just two files to install: + +* seq_primer_clip.py (the Python script) +* seq_primer_clip.xml (the Galaxy tool definition) + +The suggested location is a new tools/seq_primer_clip folder. You will also +need to modify the tools_conf.xml file to tell Galaxy to offer the tool:: + + <tool file="seq_primer_clip/seq_primer_clip.xml" /> + +If you wish to run the unit tests, also add this to tools_conf.xml.sample +and move/copy the test-data files under Galaxy's test-data folder. Then:: + + $ ./run_functional_tests.sh -id seq_primer_clip + +You will also need to install Biopython 1.54 or later. That's it. + + +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial version (not publicly released) +v0.0.2 - Sort primers by length (longest and therefore most specific first) +v0.0.3 - Consider missing bases at start/end of read as mismatches +v0.0.4 - Apply minimum length to sequences with no match too +v0.0.5 - Count clipped & non-matched short reads separately, length bug fixes +v0.0.6 - Added some functional tests +v0.0.7 - Added error check for bad filename arguments +v0.0.8 - Record version of Python script when run from Galaxy. + - Check for errors using Python script's return code. +v0.0.9 - Moved test data to workaround Galaxy Tool Shed limititation. +v0.0.10 - Include links to Tool Shed in help text and this README file. + - Use reStructuredText for this README file. + - Adopted standard MIT licence. + - Automated installation of Biopython dependency. + - Development moved to GitHub, https://github.com/peterjc/pico_galaxy + - Renamed folder and adopted README.rst naming. +======= ====================================================================== + + +Developers +========== + +This script and related tools were initially developed on the following hg branches: +http://bitbucket.org/peterjc/galaxy-central/src/fasta_filter +http://bitbucket.org/peterjc/galaxy-central/src/tools + +Development has now moved to a dedicated GitHub repository: +https://github.com/peterjc/pico_galaxy + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use +the following command from the Galaxy root folder:: + + $ tar -czf seq_primer_clip.tar.gz tools/seq_primer_clip/README.rst tools/seq_primer_clip/seq_primer_clip.* tools/seq_primer_clip/repository_dependencies.xml test-data/dop_primers.fasta test-data/MID4_GLZRM4E04_rnd30* + +Check this worked:: + + $ tar -tzf seq_primer_clip.tar.gz + tools/seq_primer_clip/README.rst + tools/seq_primer_clip/seq_primer_clip.xml + tools/seq_primer_clip/seq_primer_clip.py + tools/seq_primer_clip/repository_dependencies.xml + test-data/dop_primers.fasta + test-data/MID4_GLZRM4E04_rnd30.fasta + test-data/MID4_GLZRM4E04_rnd30.fastqsanger + test-data/MID4_GLZRM4E04_rnd30_fclip.fasta + test-data/MID4_GLZRM4E04_rnd30_fclip.fastqsanger + test-data/MID4_GLZRM4E04_rnd30_fclip.sff + test-data/MID4_GLZRM4E04_rnd30_frclip.fasta + test-data/MID4_GLZRM4E04_rnd30_frclip.fastqsanger + test-data/MID4_GLZRM4E04_rnd30_frclip.sff + test-data/MID4_GLZRM4E04_rnd30.sff + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE.