Mercurial > repos > peterjc > tmhmm_and_signalp

This package contains Galaxy wrappers for two standalone command line protein
analysis tools (SignalP 3.0 and THMHMM 2.0) from the Center for Biological
Sequence Analysis at the Technical University of Denmark,
http://www.cbs.dtu.dk/cbs/

To use these Galaxy wrappers you must first install the CBS command line
tools. At the time of writing both SignalP 3.0 and TMHMM 2.0 are free for
academic use.

These wrappers are copyright 2010 by Peter Cock, SCRI, UK. All rights
reserved. See the included LICENCE file for details.

Installation
============

1. Install the command line version of SignalP 3.0 and ensure it is on the
   PATH, see: http://www.cbs.dtu.dk/services/SignalP/

2. Install the command line version of TMHMM 2.0 and ensure it is on the
   PATH, see: http://www.cbs.dtu.dk/services/TMHMM/

3. Create a folder tools/protein_analysis under your Galaxy installation.

4. Copy/move the following files (from this archive) there:

tmhmm2.xml (Galaxy tool definition)
tmhmm2.py (Python wrapper script)
signalp3.xml (Galaxy tool definition)
signalp3.py (Python wrapper script)
seq_analysis_utils.py (shared Python code)
README (optional)

5. Edit your Galaxy conjuration file tool_conf.xml (to use the tools) AND
   also tool_conf.xml.sample (to run the tests) to include the two new tools
   by adding:

  <section name="Protein sequence analysis" id="protein_analysis">
    <tool file="protein_analysis/tmhmm2.xml" />
    <tool file="protein_analysis/signalp3.xml" />
  </section>

6. Copy/move the following test files (from these archive) to Galaxy
   subfolder test-data:

four_human_proteins.fasta
four_human_proteins.signalp3.tsv
four_human_proteins.tmhmm2.tsv

7. Run the Galaxy functional tests for these new wrappers with:

./run_functional_tests.sh -id tmhmm2
./run_functional_tests.sh -id signalp3

Alternatively, this should work (assuming you left the name and id as shown in
the XML file tool_conf.xml.sample):

./run_functional_tests.sh -sid Protein_sequence_analysis-protein_analysis

8. Restart Galaxy and check the new tools are shown and work.
author	peterjc
date	Tue, 07 Jun 2011 18:03:34 -0400
parents
children	3ff1dcbb9440