view dante_ltr_search.xml @ 17:d1c3b29b1478 draft

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<tool id="dante_ltr_search" name="DANTE_LTR retrotransposon identification" version="@TOOL_VERSION@" python_template_version="3.5">
    <macros>
        <import>macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <command detect_errors="exit_code"><![CDATA[
        echo \$PATH
        &&
        dante_ltr --gff3 '$dante'
          --reference_sequence '$reference' -M $max_missing --output output --cpu \${GALAXY_SLOTS:-1}
        &&
        mv output.gff3 $te_ltr_gff
        &&
        mv output_statistics.csv $statistics
    ]]></command>
    <inputs>
        <param type="data" name="dante" format="gff3" label="GFF3 output from DANTE pipeline - full output"/>
        <param type="data" name="reference" format="fasta" label="Reference sequence matching DANTE output" />
        <param type="integer" name="max_missing" min="0" max="3" value="1" label="Maximum number of missing protein domains to tolerate in full length retrotransposon" />
    </inputs>
    <outputs>
        <data name="te_ltr_gff" format="gff3" label="LTR retrotransposons annotation (GFF3)
        based on DANTE annotation $dante.hid and reference $reference.hid" />
        <data name="statistics" format="tabular" label="LTR retrotransposons detection
        summary based on $dante.hid and reference $reference.hid" />
    </outputs>
    <help><![CDATA[
        This tool uses output from DANTE annotation pipeline to identify full length LTR
        transposable elements. Output is in the GFF3 format and include annotation of
        5' and 3' Long Terminal Repeats, Target Site Duplication (TSD) and primer binding site (PBS).

        All identified elements contains set of protein domains as defined in
        REXdb_. Based on the results of detection of structural features,
        elements falls into five categories:

        - elements with domains, 5'LTR, 3'LTR, TSD and PBS - rank DLTP
        - elements with domains, 5'LTR, 3'LTR and PBS (TSD was not found) rank DLP
        - elements with domains, 5' LTR, 3'LTR, TSD (PBS was not found) - rank DTL
        - elements with protein domains, 5'LTR and 3'LTR (PBS and LDS were not found)  - rank DL
        - elements as cluster of proteins domains with same classification, no LTRs - rank D

       .. _REXdb: https://doi.org/10.1186/s13100-018-0144-1

      Principles of detection of LTR retrotransposons is describet in github_ repository of this tool.

      .. _github: https://github.com/kavonrtep/dante_ltr


    ]]></help>
</tool>