Mercurial > repos > petr-novak > repeatexplorer2_cerit
comparison repex_full_clustering.xml @ 3:67964b619af8 draft
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author | petr-novak |
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date | Wed, 22 Apr 2020 02:29:47 -0400 |
parents | 968f0867acc5 |
children | d89d4fa8a17b |
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2:968f0867acc5 | 3:67964b619af8 |
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1 <tool id="repeatexplorer2" name="RepeatExplorer2 clustering: " version="2.3.7" > | 1 <tool id="repeatexplorer2" name="RepeatExplorer2 clustering: " version="2.3.8" > |
2 <stdio> | 2 <stdio> |
3 <regex match="lastdb: can't open file: NEAR" source="stderr" level="fatal" description="Version of last is too old, use ver 956 or higher\n" /> | 3 <regex match="lastdb: can't open file: NEAR" source="stderr" level="fatal" description="Version of last is too old, use ver 956 or higher\n" /> |
4 <regex match="Traceback" source="stderr" level="fatal" description="Unknown error" /> | 4 <regex match="Traceback" source="stderr" level="fatal" description="Unknown error" /> |
5 <regex match="error" source="stderr" level="fatal" description="Unknown error" /> | 5 <regex match="error" source="stderr" level="fatal" description="Unknown error" /> |
6 <regex match="Warning" source="stderr" level="warning" description="Unknown error" /> | 6 <regex match="Warning" source="stderr" level="warning" description="Unknown error" /> |
10 <requirements> | 10 <requirements> |
11 <requirement type="package">last</requirement> | 11 <requirement type="package">last</requirement> |
12 <requirement type="package">imagemagick</requirement> | 12 <requirement type="package">imagemagick</requirement> |
13 <requirement type="package">mafft</requirement> | 13 <requirement type="package">mafft</requirement> |
14 <requirement type="package">blast</requirement> | 14 <requirement type="package">blast</requirement> |
15 <requirement type="package">diamond</requirement> | 15 <requirement type="package" version="0.9.29" >diamond</requirement> |
16 <requirement type="package">blast-legacy</requirement> | 16 <requirement type="package">blast-legacy</requirement> |
17 <requirement type="package">r-igraph</requirement> | 17 <requirement type="package">r-igraph</requirement> |
18 <requirement type="package">r-data.tree</requirement> | 18 <requirement type="package">r-data.tree</requirement> |
19 <requirement type="package">r-stringr</requirement> | 19 <requirement type="package">r-stringr</requirement> |
20 <requirement type="package">r-r2html</requirement> | 20 <requirement type="package">r-r2html</requirement> |
28 <requirement type="package">r-optparse</requirement> | 28 <requirement type="package">r-optparse</requirement> |
29 <requirement type="package">r-dbi</requirement> | 29 <requirement type="package">r-dbi</requirement> |
30 <requirement type="package">r-rsqlite</requirement> | 30 <requirement type="package">r-rsqlite</requirement> |
31 <requirement type="package">r-rserve</requirement> | 31 <requirement type="package">r-rserve</requirement> |
32 <requirement type="package">bioconductor-biostrings</requirement> | 32 <requirement type="package">bioconductor-biostrings</requirement> |
33 <requirement type="package" version="2.3.7">repex_tarean</requirement> | 33 <requirement type="package" version="2.3.8">repex_tarean</requirement> |
34 <requirement type="set_environment">REPEX</requirement> | 34 <requirement type="set_environment">REPEX</requirement> |
35 <requirement type="set_environment">REPEX_VERSION</requirement> | 35 <requirement type="set_environment">REPEX_VERSION</requirement> |
36 <requirement type="package" version="0.9.1" >pyrserve</requirement> | 36 <requirement type="package" version="0.9.1" >pyrserve</requirement> |
37 </requirements> | 37 </requirements> |
38 <command > | 38 <command > |
39 export PYTHONHASHSEED=0; | 39 export PYTHONHASHSEED=0; |
40 \${REPEX}/seqclust --sample ${sample} --output_dir=tarean_output --logfile=${log} --cleanup $paired --taxon $taxon | 40 \${REPEX}/seqclust --sample ${read_sampling.sample} --output_dir=tarean_output --logfile=${log} --cleanup $paired --taxon $taxon |
41 | 41 |
42 #if $advanced_options.advanced: | 42 #if $advanced_options.advanced: |
43 --mincl $advanced_options.size_threshold $advanced_options.keep_names $advanced_options.automatic_filtering -D $advanced_options.blastx.options_blastx | 43 --mincl $advanced_options.size_threshold $advanced_options.keep_names $advanced_options.automatic_filtering -D $advanced_options.blastx.options_blastx |
44 --assembly_min $advanced_options.assembly_min_cluster_size | 44 --assembly_min $advanced_options.assembly_min_cluster_size |
45 | 45 |
80 <inputs> | 80 <inputs> |
81 <param name="FastaFile" label="NGS reads" type="data" format="fasta" | 81 <param name="FastaFile" label="NGS reads" type="data" format="fasta" |
82 help="Input file must contain FASTA-formatted NGS reads. Illumina paired-end reads are recommended."/> | 82 help="Input file must contain FASTA-formatted NGS reads. Illumina paired-end reads are recommended."/> |
83 <param name="paired" type="boolean" truevalue="--paired" falsevalue="" checked="True" label="Paired-end reads" help="If paired-end reads are used, left- and right-hand reads must be interlaced and all pairs must be complete. Example of the correct format is provided in the help below." /> | 83 <param name="paired" type="boolean" truevalue="--paired" falsevalue="" checked="True" label="Paired-end reads" help="If paired-end reads are used, left- and right-hand reads must be interlaced and all pairs must be complete. Example of the correct format is provided in the help below." /> |
84 | 84 |
85 <param name="sample" label="Sample size" type="integer" value="500000" min="10000"/> | 85 <conditional name="read_sampling"> |
86 <param name="do_sampling" type="boolean" truevalue="true" falsevalue="false" checked="True" label="Use maximum processable sequence reads" /> | |
87 <when value="true"> | |
88 <!-- pass --> | |
89 <param name="sample" label="Sample size" hidden="True" type="integer" value="0" help="Number of sequence reads for clustering"/> | |
90 </when> | |
91 <when value="false"> | |
92 <param name="sample" label="Sample size" type="integer" value="500000" min="10000" help="Number of reads for clustering"/> | |
93 </when> | |
94 </conditional> | |
95 | |
96 | |
86 <param name="taxon" label="Select taxon and protein domain database version (REXdb)" type="select" help="Reference database of transposable element protein domains - REXdb - is used for annotation of repeats"> | 97 <param name="taxon" label="Select taxon and protein domain database version (REXdb)" type="select" help="Reference database of transposable element protein domains - REXdb - is used for annotation of repeats"> |
87 <option value="VIRIDIPLANTAE3.0" selected="true">Viridiplantae version 3.0 </option> | 98 <option value="VIRIDIPLANTAE3.0" selected="true">Viridiplantae version 3.0 </option> |
88 <option value="VIRIDIPLANTAE2.2" selected="true">Viridiplantae version 2.2</option> | 99 <option value="VIRIDIPLANTAE2.2" selected="true">Viridiplantae version 2.2</option> |
89 <option value="METAZOA3.0" >Metazoa version 3.0</option> | 100 <option value="METAZOA3.0" >Metazoa version 3.0</option> |
90 <option value="METAZOA2.0" >Metazoa version 2.0</option> | 101 <option value="METAZOA2.0" >Metazoa version 2.0</option> |
115 </param> | 126 </param> |
116 </conditional> | 127 </conditional> |
117 | 128 |
118 <conditional name="options"> | 129 <conditional name="options"> |
119 <param name="options" type="select" label="Similarity search options" help="Different similarity search parameters are used depending on the input data to adjust for differences in read length and error rate"> | 130 <param name="options" type="select" label="Similarity search options" help="Different similarity search parameters are used depending on the input data to adjust for differences in read length and error rate"> |
120 <option value="ILLUMINA" selected="true">Illumina reads, read length 100nt or more </option> | 131 <option value="ILLUMINA" selected="true">Illumina reads, optimized for read length 100 nt or more (search parameters: mgblast, min PID 90, W18) </option> |
121 <option value="ILLUMINA_SHORT" selected="false">Illumina reads, shorter than 100nt (Do not use reads shorter than 50nt!) </option> | 132 <option value="ILLUMINA_DUST_OFF" selected="false">Illumina reads, no masking of low complexity repeats (search parameters: mgblast, min PID 90, -W18, -F F) </option> |
122 <option value="ILLUMINA_DUST_OFF" selected="false">Illumina reads, no masking of low complexity repeats </option> | 133 <option value="ILLUMINA_SENSITIVE_MGBLAST" selected="false">Illumina reads, sensitive search (search parameters: mgblast, min PID 80, -W8) slow, experimental feature!</option> |
134 <option value="ILLUMINA_SENSITIVE_BLASTPLUS" selected="false">Illumina reads, more sensitive search (search parameters: blastn, min PID 80, -W6) extremely slow, experimental feature!</option> | |
123 <option value="OXFORD_NANOPORE" selected="false"> | 135 <option value="OXFORD_NANOPORE" selected="false"> |
124 Pseudo short reads simulated from Oxford Nanopore data (experimental feature) | 136 Pseudo short reads simulated from Oxford Nanopore data, experimental feature! |
125 </option> | 137 </option> |
126 </param> | 138 </param> |
127 </conditional> | 139 </conditional> |
128 | 140 |
129 <conditional name="custom_library"> | 141 <conditional name="custom_library"> |