Mercurial > repos > pjbriggs > macs21
diff README.rst @ 0:06cb587a5e87 draft
Uploaded initial version 2.1.0-4.
author | pjbriggs |
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date | Tue, 30 Jun 2015 08:16:18 -0400 |
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children | 02a01ea54722 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Tue Jun 30 08:16:18 2015 -0400 @@ -0,0 +1,99 @@ +MACS21: Model-based Analysis of ChIP-Seq (MACS 2.1.0) peak calling +================================================================== + +Galaxy tool wrapper for the peak calling function of MACS 2.1.0. MACS has been +developed by Tao Lui +https://github.com/taoliu/MACS/ + +The reference for MACS is: + +- Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers + RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. + 2008;9(9):R137 + +Automated installation +====================== + +Installation via the Galaxy Tool Shed will take of installing the tool wrapper and +the MACS 2.1.0 program. + +Manual Installation +=================== + +There are two files to install: + +- ``macs21_wrapper.xml`` (the Galaxy tool definition) +- ``macs21_wrapper.py.sh`` (the Python script wrapper) + +The suggested location is in a ``tools/macs21/`` folder. You will then +need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool +by adding the line: + + <tool file="macs21/macs21_wrapper.xml" /> + +You will also need to install MACS 2.1.0 and its dependencies: + +- https://pypi.python.org/pypi/MACS2 + +and ensure that it's on your Galaxy user's ``PATH`` when running the tool. + +If you want to run the functional tests, copy the sample test files under +sample test files under Galaxy's ``test-data/`` directory. Then: + + ./run_tests.sh -id macs2_wrapper + + +History +======= + +This tool was originally based on the ``modencode-dcc`` MACS2 tool developed +by Ziru Zhou (ziruzhou@gmail.com), specifically the ``16:14f378e35191`` +revision of the tool available via + +- http://toolshed.g2.bx.psu.edu/view/modencode-dcc/macs2 + +This version has been substantially modified both to adapt it to MACS 2.1.0, and +to re-implement the internal workings of the tool to conform with current +practices in invoking commands from Galaxy, and to add new functionality. + +========== ====================================================================== +Version Changes +---------- ---------------------------------------------------------------------- +2.1.0-4 - Remove 'bdgcmp' functionality. +2.1.0-3 - Add tool tests +2.1.0-2 - Add option to create bigWig file from bedGraphs; fix bug with -B + option; make --mfold defaults consistent. +2.1.0-1 - Initial version +========== ====================================================================== + + +Developers +========== + +This tool is developed on the following GitHub repository: +https://github.com/fls-bioinformatics-core/galaxy-tools/tree/master/macs21 + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use +the ``package_macs21_wrapper.sh`` script. + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE.