diff README.rst @ 1:2bd7cdbb6228 draft

Add README and citation tags.
author pjbriggs
date Tue, 09 Dec 2014 09:41:33 -0500
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+Trimmomatic: flexible read trimming tool for Illumina NGS data
+==============================================================
+
+Galaxy tool wrapper for the Trimmomatic program, which provides various functions for
+manipluating Illumina FASTQ files (both single and paired-end).
+
+Trimmomatic has been developed within Bjorn Usadel's group at RWTH Aachen university
+http://www.usadellab.org/cms/index.php?page=trimmomatic
+
+The reference for Trimmomatic is:
+
+- Bolger, A.M., Lohse, M., & Usadel, B. (2014). Trimmomatic: A flexible trimmer
+  for Illumina Sequence Data. Bioinformatics, btu170.
+
+Automated installation
+======================
+
+Installation via the Galaxy Tool Shed will take of installing the tool wrapper and
+the trimmomatic program and data, and setting the appropriate environment variables.
+
+Manual Installation
+===================
+
+There are two files to install:
+
+- ``trimmomatic.xml`` (the Galaxy tool definition)
+- ``trimmomatic.sh`` (the shell script wrapper)
+
+The suggested location is in a ``tools/trimmomatic/`` folder. You will then
+need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool
+by adding the line:
+
+    <tool file="trimmomatic/trimmomatic.xml" />
+
+You will also need to install trimmomatic 0.32:
+
+- http://www.usadellab.org/cms/uploads/supplementary/Trimmomatic/Trimmomatic-0.32.zip
+
+The tool wrapper uses the following environment variables in order to find the
+appropriate files:
+
+- ``TRIMMOMATIC_DIR`` should point to the directory holding the
+  ``trimmomatic-0.32.jar`` file
+- ``TRIMMOMATIC_ADAPTERS_DIR`` should point to the directory holding the adapter
+  sequence files (used by the ``ILLUMINACLIP`` option).
+
+If you want to run the functional tests, copy the sample test files under
+sample test files under Galaxy's ``test-data/`` directory. Then:
+
+    ./run_tests.sh -id trimmomatic
+
+You will need to have set the environment variables above.
+
+History
+=======
+
+========== ======================================================================
+Version    Changes
+---------- ----------------------------------------------------------------------
+0.32.1     - Remove ``trimmomatic_adapters.loc.sample`` and hard-code adapter files
+             into the XML wrapper.
+0.32.0     - Add tool_dependencies.xml to install Trimmomatic 0.32 automatically and
+             set the environment.
+           - Update tool versioning to use Trimmomatic version number (i.e. ``0.32``)
+             with tool iteration appended (i.e. ``.1``).
+0.0.4      - Specify '-threads 6' in <command> section.
+0.0.3      - Added MINLEN, LEADING, TRAILING, CROP and HEADCROP options of trimmomatic.
+0.0.2      - Updated ILLUMINACLIP option to use standard adapter sequences (requires
+             the trimmomatic_adapters.loc file; sample version is supplied) plus
+             cosmetic updates to wording and help text for some options.
+0.0.1      - Initial version
+========== ======================================================================
+
+
+Developers
+==========
+
+This tool is developed on the following GitHub repository:
+https://github.com/fls-bioinformatics-core/galaxy-tools/tree/master/trimmomatic
+
+For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use
+the ``package_trimmomatic.sh`` script.
+
+
+Licence (MIT)
+=============
+
+Permission is hereby granted, free of charge, to any person obtaining a copy
+of this software and associated documentation files (the "Software"), to deal
+in the Software without restriction, including without limitation the rights
+to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
+copies of the Software, and to permit persons to whom the Software is
+furnished to do so, subject to the following conditions:
+
+The above copyright notice and this permission notice shall be included in
+all copies or substantial portions of the Software.
+
+THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
+IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
+FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
+AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
+LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
+OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN
+THE SOFTWARE.