Mercurial > repos > plus91-technologies > softsearch
comparison README @ 29:79f80a903c8f draft default tip
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| author | plus91-technologies |
|---|---|
| date | Thu, 19 Jun 2014 02:43:07 -0400 |
| parents | da3c9b9fb992 |
| children |
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| 28:da3c9b9fb992 | 29:79f80a903c8f |
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| 1 SoftSearch: | 1 SoftSearch: |
| 2 | 2 |
| 3 --SoftSearch is a tools that uses soft-masked reads and discordant read pairs to identify structural variation(SV) in | 3 --SoftSearch is a tool that uses soft-masked reads and discordant read pairs to identify structural variants(SV) in |
| 4 illumina next-generation sequencing data. | 4 Illumina paired-end next-generation sequencing data. |
| 5 --It is a Integration of Multiple Sequence Features to Identify Breakpoints of Structural Variations. | 5 --Using Soft Clipped reads in which one part of the read maps to the reference genome but the other part does not SoftSearch |
| 6 --Breakpoint is a place or time at which an interruption or change is made. | 6 can identify and call SVs including deletions, insertions, translocations, inversions, and tandem duplications |
| 7 --SoftSearch requires only two inputs: a BAM file of aligned reads and fasta file of the genome to which the reads were aligned. | 7 --SoftSearch requires two inputs: a BAM file of aligned reads and fasta file of the reference genome to which the reads |
| 8 were aligned. | |
| 8 | 9 |
| 9 Google Code Link: | 10 Google Code Link: |
| 10 | 11 |
| 11 https://code.google.com/p/softsearch/ | 12 https://code.google.com/p/softsearch/ |
| 12 | 13 |
| 14 Pre-Requirement: | 15 Pre-Requirement: |
| 15 | 16 |
| 16 -Perl | 17 -Perl |
| 17 -Bedtools | 18 -Bedtools |
| 18 -SamTools | 19 -SamTools |
| 19 Must have an entry in environment variable | 20 Must be accessible from the PATH environment variable |
| 20 | 21 |
| 21 ------------------------------------------------------------------------------------------------------------------ | 22 ------------------------------------------------------------------------------------------------------------------ |
| 22 How To Selecte fasta file from Database (Additional Configuration): | 23 How To Select fasta file from Database (Additional Configuration): |
| 23 | 24 |
| 24 --Select fasta_indexes.loc file for that go to shed_tools/toolshed.g2.bx.psu.edu/repos/plus91-technologies/softsearch/ | 25 --A fasta_indexes.loc file must be located in shed_tools/toolshed.g2.bx.psu.edu/repos/plus91-technologies/softsearch/ |
| 25 8578d978014c/softsearch/tool-data/ | 26 8578d978014c/softsearch/tool-data/ |
| 26 | 27 |
| 27 --open the fasta_indexes.loc file and make changes like | 28 --open the fasta_indexes.loc file and add an entry according to the example below |
| 28 | 29 |
| 29 <unique_build_id> <dbkey> <display_name> <file_base_path> | 30 <unique_build_id> <dbkey> <display_name> <file_base_path> |
| 30 | 31 |
| 31 eg: hg19full hg19 Human (Homo sapiens): hg19 Full /depot/data2/galaxy/hg19/sam/hg19full.fa | 32 eg: hg19full hg19 Human (Homo sapiens): hg19 Full /depot/data2/galaxy/hg19/sam/hg19full.fa |
| 32 | 33 |
| 33 --open shed_tool_data_table_conf.xml file and add following. | 34 --open shed_tool_data_table_conf.xml file and add the following |
| 34 | 35 |
| 35 <tables> | 36 <tables> |
| 36 <table name="fasta_indexes" comment_char="#"> | 37 <table name="fasta_indexes" comment_char="#"> |
| 37 <columns>value, dbkey, name, path</columns> | 38 <columns>value, dbkey, name, path</columns> |
| 38 <file path="/path/to/fasta_indexes.loc" /> | 39 <file path="/path/to/fasta_indexes.loc" /> |
