Mercurial > repos > portiahollyoak > temp
annotate scripts/pickUniqPos.pl @ 21:9672fe07a232 draft default tip
planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
author | mvdbeek |
---|---|
date | Mon, 05 Dec 2016 09:58:47 -0500 |
parents | e19d9742c99b |
children |
rev | line source |
---|---|
0
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
1 #!/share/bin/perl |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
2 use Bio::Seq; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
3 use List::Util qw(sum); |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
4 |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
5 die "perl $0 <sam>\n" if @ARGV<1; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
6 open in,$ARGV[0]; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
7 my %pe; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
8 while(<in>) |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
9 { |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
10 chomp; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
11 my @f=split/\t/,$_,12; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
12 ## read number 1 or 2 |
21
9672fe07a232
planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents:
11
diff
changeset
|
13 #my ($rnum)=$f[1]=~/(\d)$/; |
9672fe07a232
planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents:
11
diff
changeset
|
14 my $rnum=1; |
9672fe07a232
planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents:
11
diff
changeset
|
15 if (($f[1] & 128) == 128) {$rnum=2;} |
0
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
16 |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
17 ## XT:A:* |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
18 my ($xt)=$f[11]=~/XT:A:(.)/; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
19 |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
20 my $strand="+"; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
21 ## revcomp |
21
9672fe07a232
planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty
mvdbeek
parents:
11
diff
changeset
|
22 if(($f[1] & 16) == 16) |
0
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
23 { |
11
e19d9742c99b
planemo upload for repository https://github.com/portiahollyoak/Tools commit bfcc36291d97d1c0986f034ca1a03433e8299ff2-dirty
portiahollyoak
parents:
0
diff
changeset
|
24 my $seq=Bio::Seq->new(-seq=>$f[9], -alphabet => 'dna'); |
0
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
25 $f[9]=$seq->revcom->seq; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
26 $strand="-"; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
27 } |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
28 |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
29 ## parse CIGAR |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
30 if($xt eq "U") |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
31 { |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
32 # CIGAR |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
33 my (@cigar_m)=$f[5]=~/(\d+)M/g; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
34 my (@cigar_d)=$f[5]=~/(\d+)D/g; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
35 my (@cigar_s)=$f[5]=~/(\d+)S/g; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
36 my (@cigar_i)=$f[5]=~/(\d+)I/g; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
37 my $aln_ln=sum(@cigar_m,@cigar_d); |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
38 |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
39 print $f[2],"\t",$f[3]-1,"\t",$f[3]-1+$aln_ln,"\t$f[0]/$rnum\t",$f[9],"\t",$strand,"\n"; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
40 } |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
41 } |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
42 close in; |
28d1a6f8143f
planemo upload for repository https://github.com/portiahollyoak/Tools commit 132bb96bba8e7aed66a102ed93b7744f36d10d37-dirty
portiahollyoak
parents:
diff
changeset
|
43 |