annotate scripts/pickUniqPos.pl @ 21:9672fe07a232 draft default tip

planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty

#!/share/bin/perl
use Bio::Seq;
use List::Util qw(sum);

die "perl $0 <sam>\n" if @ARGV<1;
open in,$ARGV[0];
my %pe;
while(<in>)
{
	chomp;
	my @f=split/\t/,$_,12;
	## read number 1 or 2
	#my ($rnum)=$f[1]=~/(\d)$/;
        my $rnum=1;
        if (($f[1] & 128) == 128) {$rnum=2;}

	## XT:A:* 
	my ($xt)=$f[11]=~/XT:A:(.)/;

	my $strand="+";
	## revcomp
	if(($f[1] & 16) == 16)
        {
                my $seq=Bio::Seq->new(-seq=>$f[9], -alphabet => 'dna');
                $f[9]=$seq->revcom->seq;
		$strand="-";
        }

	## parse CIGAR
	if($xt eq "U")
        {
                # CIGAR
                my (@cigar_m)=$f[5]=~/(\d+)M/g;
                my (@cigar_d)=$f[5]=~/(\d+)D/g;
                my (@cigar_s)=$f[5]=~/(\d+)S/g;
                my (@cigar_i)=$f[5]=~/(\d+)I/g;
                my $aln_ln=sum(@cigar_m,@cigar_d);
		
		print $f[2],"\t",$f[3]-1,"\t",$f[3]-1+$aln_ln,"\t$f[0]/$rnum\t",$f[9],"\t",$strand,"\n";
	}
}
close in;