Mercurial > repos > portiahollyoak > temp
comparison scripts/pickUniqPairFastq_MEM.pl @ 12:ca36262102d8 draft
planemo upload for repository https://github.com/portiahollyoak/Tools commit 5d021f520b653582862ec98dd812a051b804aa50
author | portiahollyoak |
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date | Fri, 29 Apr 2016 05:47:54 -0400 |
parents | 28d1a6f8143f |
children | 9672fe07a232 |
comparison
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11:e19d9742c99b | 12:ca36262102d8 |
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28 elsif ($a[$i] =~ /^XS:i:/) { | 28 elsif ($a[$i] =~ /^XS:i:/) { |
29 $a[$i] =~ s/XS:i://; | 29 $a[$i] =~ s/XS:i://; |
30 $xs=$a[$i]; | 30 $xs=$a[$i]; |
31 } | 31 } |
32 if (($xs > 0) && ($as-$xs <= $ARGV[2])) {$xt="R";} | 32 if (($xs > 0) && ($as-$xs <= $ARGV[2])) {$xt="R";} |
33 else {$xt="U";} | 33 elsif ($as > 0) {$xt="U";} |
34 } | 34 } |
35 | 35 |
36 ## revcom the read mapped to the reverse strand | 36 ## revcom the read mapped to the reverse strand |
37 if($f[1]=~/r/) | 37 if($f[1]=~/r/) |
38 { | 38 { |
39 my $seq=Bio::Seq->new(-seq=>$f[9]); | 39 my $seq=Bio::Seq->new(-seq=>$f[9], -alphabet => 'dna'); |
40 $f[9]=$seq->revcom->seq; | 40 $f[9]=$seq->revcom->seq; |
41 $f[10]=reverse $f[10]; | 41 $f[10]=reverse $f[10]; |
42 } | 42 } |
43 if (($rnum == 1) || ($rnum == 2)) | 43 if (($rnum == 1) || ($rnum == 2)) |
44 { | 44 { |