view scripts/pickUniqPairFastq_MEM.pl @ 21:9672fe07a232 draft default tip

planemo upload for repository https://github.com/portiahollyoak/Tools commit 0fea84d05f8976b8360a8b4943ecb01b87e3ade0-dirty

#!/share/bin/perl
use Bio::Seq;

die "perl $0 <sam> <output prefix>\n" if @ARGV<1;

open m1,">$ARGV[1].1.fastq";
open m2,">$ARGV[1].2.fastq";

open in,$ARGV[0];
my %pe;
while(<in>)
{
	chomp;
	my @f=split/\t/,$_,12;
	## read number 1 or 2
	#my ($rnum)=$f[1]=~/(\d)$/;
        my $rnum=1;
        if (($f[1] & 128) == 128) {$rnum=2;}

	## XT:A:* 
	my $xt="";
	my @a=split(/\s+/, $_);
	my $as=0;
	my $xs=0;
	for my $i (11..$#a) {
	    if ($a[$i] =~ /^AS:i:/) {
		$a[$i] =~ s/AS:i://;
		$as=$a[$i];
	    }
	    elsif ($a[$i] =~ /^XS:i:/) {
		$a[$i] =~ s/XS:i://;
		$xs=$a[$i];
	    }
	    if (($xs > 0) && ($as-$xs <= $ARGV[2])) {$xt="R";}
	    elsif ($as > 0) {$xt="U";}
	}

	## revcom the read mapped to the reverse strand
	if (($f[1] & 16) == 16)
	{
		my $seq=Bio::Seq->new(-seq=>$f[9], -alphabet => 'dna');
		$f[9]=$seq->revcom->seq;
		$f[10]=reverse $f[10];
	}
	if (($rnum == 1) || ($rnum == 2))
	{
	    ${$pe{$f[0]}}[$rnum-1]=[$xt,$f[9],$f[10]];
	}
}
close in;

foreach my $id (keys %pe)
{
	my @rid=@{$pe{$id}};
	if (($rid[0][1] ne "") && ($rid[1][1] ne "") && (($rid[0][0] eq "U" || $rid[1][0] eq "U")))
	{
		print m2 "@"."$id/2","\n",$rid[1][1],"\n","+$id/2","\n",$rid[1][2],"\n";
		print m1 "@"."$id/1","\n",$rid[0][1],"\n","+$id/1","\n",$rid[0][2],"\n";
	}
}
close m1;
close m2;